The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
DNA base modifications in chromatin of human cancerous tissues
Abstract
Free radical‐induced damage to DNA in vivo is implicated to play a role in carcinogenesis. Evidence exists that DNA damage by endogenous free radicals occurs in vivo, and there is a steady‐state level of free radical‐modified bases in cellular DNA. We have investigated endogenous levels of typical free radical‐induced DNA base modifications in chromatin of various human cancerous tissues and their cancer‐free surrounding tissues. Five different types of surgically removed tissues were used, namely colon, stomach, ovary, brain and lung tissues. In chromatin samples isolated from these tissues, five pyrimidine‐derived and six purine‐derived modified DNA bases were identified and quantitated by gas chromatography/mass spectrometry with selected‐ion monitoring. These were 5‐hydroxy‐5‐methylhydantoin, 5‐hydroxyhydantoin, 5‐(hydroxymethyl)uracil, 5‐hydroxycytosine, 5,6‐dihydroxycytosine, 4,6‐diamino‐5‐formamidopyrimidine, 8‐hydroxyadenine, xanthine, 2‐hydroxyadenine, 2,6‐diamino‐4‐hydroxy‐5‐formamidopyrimidine, and 8‐hydroxyguanine. These compounds are known to be formed typically by hydroxyl radical attack an DNA bases. In all cases, elevated amounts over control levels of modified DNA bases were found in cancerous tissues. The amounts modified bases depended on the tissue type. Lung tissues removed from smokers had the highest increases of modified bases above the control levels, and the highest overall amounts. Colon cancer tissue samples had the lowest increases of modified buses over the control levels. The results clearly indicate higher steady‐state levels of modified DNA bases in cancerous tissues than in their cancer‐free surrounding tissues. Some of these lesions are known to be promutagenic, although others have not been investigated for their mutagenicity. Identified DNA lesions may play a causative role in carcinogenesis.
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Citations
Cites
Modification of DNA bases in mammalian chromatin by radiation-generated free radicals
Gajewski, E., Rao, G., Nackerdien, Z., & Dizdaroglu, M. (1990) · Biochemistry · doi:10.1021/bi00486a014 · Cited by 258
Cited by
Oxidative DNA damage: mechanisms, mutation, and disease
Cooke, M. S., Evans, M. D., Dizdaroglu, M., & Lunec, J. (2003) · The FASEB Journal · doi:10.1096/fj.02-0752rev · Cited by 3,245
WISEMAN, H., & HALLIWELL, B. (1996) · Biochemical Journal · doi:10.1042/bj3130017 · Cited by 2,345
Oxidative DNA damage and disease: induction, repair and significance
Evans, M. D., Dizdaroglu, M., & Cooke, M. S. (2004) · Mutation Research/Reviews in Mutation Research · doi:10.1016/j.mrrev.2003.11.001 · Cited by 1,372
Persistent oxidative stress in cancer
Toyokuni, S., Okamoto, K., Yodoi, J., & Hiai, H. (1995) · FEBS Letters · doi:10.1016/0014-5793(94)01368-b · Cited by 1,219
Role of oxygen free radicals in cancer development
Dreher, D., & Junod, A. F. (1996) · European Journal of Cancer · doi:10.1016/0959-8049(95)00531-5 · Cited by 855
Metadata
- Authors
- Olinski, R., Zastawny, T., Budzbon, J., Skokowski, J., Zegarski, W., & Dizdaroglu, M.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1992-09-07
- Volume
- 309
- Issue
- 2
- Pages
- 193-198
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2089510123
- Cited by
- 284
- References
- 1
- FWCI
- 6.32
- Type
- journal article
- Language
- en
- Open access
- closed
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- Olinski, R., Zastawny, T., Budzbon, J., Skokowski, J., Zegarski, W., & Dizdaroglu, M. (1992). DNA base modifications in chromatin of human cancerous tissues. FEBS Letters. https://doi.org/10.1016/0014-5793(92)81093-2
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
