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Botulinum neurotoxins serotypes A and E cleave SNAP‐25 at distinct COOH‐terminal peptide bonds
Abstract
SNAP‐25, a membrane‐associated protein of the nerve terminal, is specifically cleaved by botulinum neurotoxins serotypes A and E, which cause human and animal botulism by blocking neurotransmitter release at the neuromuscular junction. Here we show that these two metallo‐endopeptidase toxins cleave SNAP‐25 at two distinct carboxyl‐terminal sites. Serotype A catalyses the hydrolysis of the Gln 197‐Arg 198 peptide bond, while serotype E cleaves the Arg 180‐Ile 181 peptide linkage. These results indicate that the carboxyl‐terminal region of SNAP‐25 plays a crucial role in the multi‐protein complex that mediates vesicle docking and fusion at the nerve terminal.
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Citations
Cited by
Burgoyne, R. D., & Morgan, A. (2003) · Physiological Reviews · doi:10.1152/physrev.00031.2002 · Cited by 934
SV2 Is the Protein Receptor for Botulinum Neurotoxin A
Dong, M., Yeh, F., Tepp, W. H., Dean, C., Johnson, E. A., Janz, R., & Chapman, E. R. (2006) · Science · doi:10.1126/science.1123654 · Cited by 770
Membrane Repair: Mechanisms and Pathophysiology
Cooper, S. T., & McNeil, P. L. (2015) · Physiological Reviews · doi:10.1152/physrev.00037.2014 · Cited by 386
Clostridial neurotoxins: new tools for dissecting exocytosis
Niemann, H., Blasi, J., & Jahn, R. (1994) · Trends in Cell Biology · doi:10.1016/0962-8924(94)90203-8 · Cited by 326
Structure and function of SNARE and SNARE-interacting proteins
Brunger, A. T. (2005) · Quarterly Reviews of Biophysics · doi:10.1017/s0033583505004051 · Cited by 309
Metadata
- Authors
- Schiavo, G., Santucci, A., Dasgupta, B. R., Mehta, P. P., Jontes, J., Benfenati, F., Wilson, M. C., & Montecucco, C.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1993-11-29
- Volume
- 335
- Issue
- 1
- Pages
- 99-103
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W1963786196
- Cited by
- 438
- References
- 0
- FWCI
- 17.77
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Botulinum neurotoxins serotypes A and E cleave SNAP‐25 at distinct COOH‐terminal peptide bonds. https://app.cochat.ai/papers/10.1016/0014-5793(93)80448-4
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- Schiavo, G., Santucci, A., Dasgupta, B. R., Mehta, P. P., Jontes, J., Benfenati, F., Wilson, M. C., & Montecucco, C. (1993). Botulinum neurotoxins serotypes A and E cleave SNAP‐25 at distinct COOH‐terminal peptide bonds. FEBS Letters. https://doi.org/10.1016/0014-5793(93)80448-4
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