The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Cloning and expression in Escherichia coli of the gene encoding β‐C‐4‐oxygenase, that converts β‐carotene to the ketocarotenoid canthaxanthin in Haematococcus pluvialis
Open accessAbstract
In the green alga Haematococcus pluvialis the ketocarotenoid astaxanthin accumulates under stress conditions. Astaxanthin is a red carotenoid pigment which possess antioxidative activity. We have cloned the gene for β‐C‐4 oxygenase (β‐carotene ketolase) from the green algae H. pluvialis. The cloning method took advantage of a strain of E. coli which was genetically engineered to produce β‐carotene. An expression cDNA library of H. pluvialis was transfected to cells of this strain and visually screened for brown‐red pigmented colonies. One colony out of 100,000 transformants showed color change due to accumulation of canthaxanthin. The cDNA clone in this transformant colony encodes the enzyme β‐C‐4 oxygenase that catalyzes the conversion of β carotene to canthaxanthin via echinenone. This enzyme does not convert zeaxanthin to astaxanthin. It is concluded that in H. pluvialis astaxanthin is synthesized via canthaxanthin and therefore an additional enzyme is predicted, which converts canthaxanthin to astaxanthin.
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Grung, M., D'Souza, F. M. L., Borowitzka, M., & Liaaen-Jensen, S. (1992) · Journal of Applied Phycology · doi:10.1007/bf02442465 · Cited by 151
Cunningham, F. X., Chamovitz, D., Misawa, N., Gantt, E., & Hirschberg, J. (1993) · FEBS Letters · doi:10.1016/0014-5793(93)80980-9 · Cited by 110
Self-citation
Cited by
Secondary ketocarotenoid astaxanthin biosynthesis in algae: a multifunctional response to stress
Lemoine, Y., & Schoefs, B. (2010) · Photosynthesis Research · doi:10.1007/s11120-010-9583-3 · Cited by 384
Metabolic engineering towards biotechnological production of carotenoids in microorganisms
P., L., & C., S.-D. (2002) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-002-1101-x · Cited by 309
New Insights into Metabolic Properties of Marine Bacteria Encoding Proteorhodopsins
Sabehi, G., Loy, A., Jung, K.-H., Partha, R., Spudich, J. L., Isaacson, T., Hirschberg, J., Wagner, M., & Béjà, O. (2005) · PLoS Biology · doi:10.1371/journal.pbio.0030273 · Cited by 256
GENETICS of EUBACTERIAL CAROTENOID BIOSYNTHESIS: A colorful tale
Armstrong, G. A. (1997) · Annual Review of Microbiology · doi:10.1146/annurev.micro.51.1.629 · Cited by 241
Travel advice on the road to carotenoids in plants
Farré, G., Sanahuja, G., Naqvi, S., Bai, C., Capell, T., Zhu, C., & Christou, P. (2010) · Plant Science · doi:10.1016/j.plantsci.2010.03.009 · Cited by 176
Metadata
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1995-05-08
- Volume
- 364
- Issue
- 2
- Pages
- 125-128
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W1989356718
- Cited by
- 176
- References
- 2
- FWCI
- 1.89
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Cloning and expression in Escherichia coli of the gene encoding β‐C‐4‐oxygenase, that converts β‐carotene to the ketocarotenoid canthaxanthin in Haematococcus pluvialis. (1995). FEBS Letters. https://doi.org/10.1016/0014-5793(95)00368-j
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