Cited by 78Glycogen Storage Diseases and MyoclonusMetabolism, Diabetes, and CancerGenomics and Chromatin Dynamics

Cloning and sequencing of the 5′ region of the human glucose‐6‐phosphatase gene: transcriptional regulation by cAMP, insulin and glucocorticoids in H4IIE hepatoma cells

Schmoll, Dieter, Allan, Bernard B., Burchell, Ann·1996·FEBS Letters

Abstract

We have cloned and sequenced the first 1.2 kb of the 5′ region of the human glucose‐6‐phosphatase gene. Transfection of H4IIE hepatoma cells with the 1.2 kb fragment fused to a luciferase reporter gene demonstrated both basal and hormone responsive luciferase activity. Dexamethasone increased and insulin decreased luciferase activity. Insulin and dibutyryl cyclic AMP both significantly decreased activity in the presence of dexamethasone.

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Crossref · 10.1016/0014-5793(96)00224-4

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27 of 27 references verified0 citation errors · 0 not checkable

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  • Glucocorticoids, specifically dexamethasone, cause a larger (≤10-fold) increase in G-6-Pase activity and mRNA in cultured hepatoma cells.

    “Glucocorticoids and specifically dexamethasone cause a larger (≤10-fold) increase in G-6-Pase activity and in the level of its mRNA in cultured hepatoma cells (33).”

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Authors
Schmoll, D., Allan, B. B., & Burchell, A.
Affiliations
University of Dundee (GB)
Corresponding author
Burchell, Ann
Venue
FEBS Letters
Publisher
Wiley
Published
1996-03-25
Volume
383
Issue
1-2
Pages
63-66
ISSN
  • 0014-5793 (ISSN-L)
  • 1873-3468
OpenAlex
W1993245642
Cited by
78
References
27
Citations by year
2026: 1 · 2023: 2 · 2022: 1 · 2020: 3 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 2 · 2015: 2 · 2014: 2 · 2013: 3 · 2012: 2
FWCI
4.93
Type
journal article
Language
en
Open access
closed
Locations
  • FEBS Letters (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Rheumatology › Glycogen Storage Diseases and Myoclonus
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
5' regulatory region, transcriptional regulation, cyclic AMP, insulin, glucocorticoids, dexamethasone, H4IIE cells, luciferase reporter
MeSH terms
Animals, Base Sequence, Cloning, Molecular, Dexamethasone, Bucladesine, Glucose-6-Phosphatase, Humans, Insulin, Liver Neoplasms, Experimental, Molecular Sequence Data, Promoter Regions, Genetic, Transcription, Genetic, Transfection, Tumor Cells, Cultured, Gene Expression Regulation, Enzymologic, Genes, Reporter, Rats

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