Cloning and sequencing of the 5′ region of the human glucose‐6‐phosphatase gene: transcriptional regulation by cAMP, insulin and glucocorticoids in H4IIE hepatoma cells
Abstract
We have cloned and sequenced the first 1.2 kb of the 5′ region of the human glucose‐6‐phosphatase gene. Transfection of H4IIE hepatoma cells with the 1.2 kb fragment fused to a luciferase reporter gene demonstrated both basal and hormone responsive luciferase activity. Dexamethasone increased and insulin decreased luciferase activity. Insulin and dibutyryl cyclic AMP both significantly decreased activity in the presence of dexamethasone.
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Glucocorticoids, specifically dexamethasone, cause a larger (≤10-fold) increase in G-6-Pase activity and mRNA in cultured hepatoma cells.
“Glucocorticoids and specifically dexamethasone cause a larger (≤10-fold) increase in G-6-Pase activity and in the level of its mRNA in cultured hepatoma cells (33).”
From Hypoglycemic effects of brassinosteroid in diet-induced obese mice (2012) · cites this paper as [33]
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- Authors
- Schmoll, D., Allan, B. B., & Burchell, A.
- Affiliations
- University of Dundee (GB)
- Corresponding author
- Burchell, Ann
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1996-03-25
- Volume
- 383
- Issue
- 1-2
- Pages
- 63-66
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W1993245642
- Cited by
- 78
- References
- 27
- Citations by year
- 2026: 1 · 2023: 2 · 2022: 1 · 2020: 3 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 2 · 2015: 2 · 2014: 2 · 2013: 3 · 2012: 2
- FWCI
- 4.93
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Rheumatology › Glycogen Storage Diseases and Myoclonus
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- 5' regulatory region, transcriptional regulation, cyclic AMP, insulin, glucocorticoids, dexamethasone, H4IIE cells, luciferase reporter
- MeSH terms
- Animals, Base Sequence, Cloning, Molecular, Dexamethasone, Bucladesine, Glucose-6-Phosphatase, Humans, Insulin, Liver Neoplasms, Experimental, Molecular Sequence Data, Promoter Regions, Genetic, Transcription, Genetic, Transfection, Tumor Cells, Cultured, Gene Expression Regulation, Enzymologic, Genes, Reporter, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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