CoChat
Sign Up
Cited by 43Gut microbiota and healthDrug Transport and Resistance MechanismsGastrointestinal motility and disorders

Effects of intestinal microbial bile salt sulfatase activity on bile salt kinetics in gnotobiotic rats

Robben, J., Caenepeel, Ph., Van Eldere, J., Eyssen, H.·1988·Gastroenterology
DOI 10.1016/0016-5085(88)90443-x
Open this paper in CoChat — free account

Abstract

Hepatic sulfation is thought to promote fecal excretion of lithocholate in humans by impairing the enterohepatic recirculation of the compound. Sulfatases produced by the gut flora may, at least in part, counteract this process. To investigate this interaction, female germfree rats, which are known to excrete considerable amounts of sulfated bile salts, were selectively associated with a bile salt desulfating flora. In these rats nearly all cecal, colonic, and fecal bile salt sulfate esters were hydrolyzed, resulting in a decrease of total fecal bile salt excretion of greater than 25% compared with gnotobiotic rats without a bile salt desulfating flora. Desulfation of allochenodeoxycholate, the major sulfated bile salt in germfree rats, led to an enhanced recirculation and 12 alpha-hydroxylation of the compound in the liver, resulting in an increased fecal output of allocholate. Microbial desulfation of intraperitoneally injected [24-14C]taurolithocholate-3-sulfate caused a fivefold decrease of the fecal plus urinary excretion rate of the isotope to approximately that found for unsulfated [24-14C]taurolithocholate. Coassociation of the gnotobiotic rats with a microflora that normalized the small intestinal transit time and cecal size led to a rise of total fecal bile salt excretion of greater than 30% and a twofold accelerated excretion of both sulfated and unsulfated injected [24-14C]taurolithocholate. We conclude that in rats the gut flora rapidly desulfates intestinal bile salt sulfates, enhancing the enterohepatic recirculation and subsequent hydroxylation of the desulfated bile salts. In addition, these data illustrate the importance of having a well-defined microflora to normalize intestinal transit time and cecal size of gnotobiotic animals when performing kinetic studies of enterohepatic circulating compounds.

Integrity signals

Registry

Crossref · 10.1016/0016-5085(88)90443-x

Cites

33 of 33 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The gut microbiota may influence functional constipation through its impact on bile acid sulfation, in which colonic bacterial bile salt sulfatase may desulfate bile acids, empowering their effect to stimulate colonic secretion and transit and consequently improve colonic fluid transport.

    “Another mechanism for the gut microbiota to influence FC is through its impact on BA sulfation, which abolishes the effect of the BAs on fluid transport [ 51 , 52 ]. BA sulfation likely occurred in hepatocytes, while colonic bacterial bile salt sulfatase may desulfate BA, empowering its effect to stimulate colonic secretion and transit, and consequently improve colonic fluid transport [ 53 ].”

    From Role of gut microbiota in functional constipation (2021) · cites this paper as [53]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Robben, J., Caenepeel, P., Van Eldere, J., & Eyssen, H.
Affiliations
Rega Institute for Medical Research (BE)
ORCID
Robben, J.
Venue
Gastroenterology
Publisher
Elsevier BV
Published
1988-02
Volume
94
Issue
2
Pages
494-502
ISSN
0016-5085 (ISSN-L)
DOI
10.1016/0016-5085(88)90443-x
OpenAlex
W1660647916
Cited by
43
References
33
Citations by year
2026: 1 · 2025: 3 · 2024: 1 · 2023: 1 · 2022: 3 · 2021: 3 · 2019: 3 · 2018: 1 · 2017: 1 · 2013: 2 · 2012: 2
FWCI
1.05
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Gastroenterology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
Keywords
gut microbiota, enterohepatic circulation, lithocholic acid, taurolithocholate, germ-free rats, gnotobiotic rats, intestinal transit time
MeSH terms
Animals, Bacteria, Bile Acids and Salts, Biotransformation, Cecum, Clostridium, Enterohepatic Circulation, Feces, Female, Germ-Free Life, Intestines, Rats, Inbred Strains, Sulfatases, Sulfates, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review