In vitro characterization of anthelmintic susceptibility of field isolates of the pig nodular worm Oesophagostomum spp., susceptible or resistant to various anthelmintics
Abstract
A larval development assay (LDA) and an egg hatch paralysis assay (EHPA) were used to measure the sensitivity to anthelmintics of eggs and larvae of nodular worms (Oesophagostomum spp.) in pigs. The tests were carried out using in vivo defined resistant and susceptible isolates of Oesophagostomum dentatum, O. quadrispinulatum and Oesophagostomum spp. For measurement of pyrantel/morantel and levamisole sensitivity the LDA was found able to distinguish between susceptible or resistant isolates of Oesophagostomum. The EHPA was able to detect levamisole resistance, but the test failed to show differences in response to pyrantel between pyrantel susceptible and resistant lines. The possible routine application of LDA and EHPA in the diagnosis of anthelmintic resistance in Oesophagostomum spp. is discussed.
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The EHA has been used successfully to detect resistance against benzimidazoles in multiple nematode parasites of livestock.
“Based on the ovicidal properties of the benzimidazole drug class [54], this assay has been used successfully to detect resistance against benzimidazoles in multiple nematode parasites of livestock [55–57].”
From Multiple drug resistance in the canine hookworm Ancylostoma caninum: an emerging threat? (2019) · cites this paper as [57]
In vitro bioassays used for detection of anthelmintic resistance in parasitic nematodes are highly species-specific and drug class-specific in their ability to provide useful levels of discrimination between susceptible and resistant isolates.
“This finding builds on previous works demonstrating that in vitro bioassays used for detection of anthelmintic resistance in parasitic nematodes are highly species-specific and drug class-specific in their ability to provide useful levels of discrimination between susceptible and resistant isolates [43, 57, 63].”
From Multiple drug resistance in the canine hookworm Ancylostoma caninum: an emerging threat? (2019) · cites this paper as [57]
Cites
Manual of veterinary parasitological laboratory techniques
Friedhoff, K. T. (1978) · Veterinary Parasitology · doi:10.1016/0304-4017(78)90013-4 · Cited by 908 · see citing works
A microlarval development assay for the detection of anthelmintic resistance in sheep nematodes
Hubert, J., & Kerbœuf, D. (1992) · Veterinary Record · doi:10.1136/vr.130.20.442 · Cited by 284 · see citing works
Chemotherapy of Parasitic Diseases
Campbell, W. C., & Rew, R. S. (1986) · Springer eBooks · doi:10.1007/978-1-4684-1233-8 · Cited by 216 · see citing works
Prichard, R. K. (1994) · Veterinary Parasitology · doi:10.1016/0304-4017(94)90094-9 · Cited by 215 · see citing works
Resistance of parasites to antiparasitic drugs
Bryant, C. R. (1992) · International Journal for Parasitology · doi:10.1016/0020-7519(92)90161-d · Cited by 152 · see citing works
Gill, J. H., Redwin, J. M., Van Wyk, J. A., & Lacey, E. (1995) · International Journal for Parasitology · doi:10.1016/0020-7519(94)00087-5 · Cited by 150 · see citing works
Resistance of Oesophagostomum spp. in pigs to pyrantel citrate
Roepstorff, A., Bjørn, H., & Nansen, P. (1987) · Veterinary Parasitology · doi:10.1016/0304-4017(87)90044-6 · Cited by 104 · see citing works
Self-citationThe mechanism of action of the anthelmintic levamisole
Coles, G. C., East, J. M., & Jenkins, S. N. (1975) · General Pharmacology The Vascular System · doi:10.1016/0306-3623(75)90030-0 · Cited by 98 · see citing works
A larval development test for the detection of anthelmintic resistance in nematodes of sheep
Taylor, M. A. (1990) · Research in Veterinary Science · doi:10.1016/s0034-5288(18)31077-4 · Cited by 96 · see citing works
Chemotherapy of Parasitic Diseases
Lw, S., Campbell, W. C., & Rew, R. S. (1987) · Journal of Parasitology · doi:10.2307/3282385 · Cited by 85 · see citing works
Larval paralysis as an in vitro assay of levamisole and morantel tartrate resistance inOstertagia
Martin, P. J., & Jambre, L. F. L. (1979) · Veterinary Research Communications · doi:10.1007/bf02268963 · Cited by 82 · see citing works
A larval development assay for the simultaneous detection of broad spectrum anthelmintic resistance
Lacey, E. (1990) · Medical Entomology and Zoology · Cited by 82 · see citing works
Larval development test for detection of anthelmintic resistant nematodes
Coles, G. C., Tritschler, J. P., GIORDANO, D. J., Laste, N. J., & SCHMIDT, A. L. (1988) · Research in Veterinary Science · doi:10.1016/s0034-5288(18)30893-2 · Cited by 81 · see citing works
Sangster, N. C., Whitlock, H. V., Russ, I. G., Gunawan, M., Griffin, D. L., & Kelly, J. D. (1979) · Research in Veterinary Science · doi:10.1016/s0034-5288(18)32867-4 · Cited by 78 · see citing works
Hunt, K., & Taylor, M. (1989) · Veterinary Record · doi:10.1136/vr.125.7.153 · Cited by 78 · see citing works
An egg-hatch assay for resistance to levamisole in trichostrongyloid nematode parasites
Dobson, R. J., Donald, A. D., Waller, P. J., & Snowdon, K. L. (1986) · Veterinary Parasitology · doi:10.1016/0304-4017(86)90034-8 · Cited by 67 · see citing works
BARTON, N. (1983) · International Journal for Parasitology · doi:10.1016/0020-7519(83)90002-4 · Cited by 67 · see citing works
Bjørn, H., Roepstorff, A., Waller, P. J., & Nansen, P. (1990) · Veterinary Parasitology · doi:10.1016/0304-4017(90)90022-4 · Cited by 58 · see citing works
Self-citationWaller, P. J., Dobson, R. J., Donald, A. D., Griffiths, D., & Smith, E. F. (1985) · International Journal for Parasitology · doi:10.1016/0020-7519(85)90014-1 · Cited by 57 · see citing works
Selection of ivermectin-resistant Trichostrongylus colubriformis in lambs
Giordano, D. J., Tritschler, J. P., & Coles, G. C. (1988) · Veterinary Parasitology · doi:10.1016/0304-4017(88)90161-6 · Cited by 45 · see citing works
Johansen, M. V. (1989) · Veterinary Research Communications · doi:10.1007/bf00402569 · Cited by 45 · see citing works
Trematode Infections of Domestic Animals
Boray, J. C. (1986) · Springer eBooks · doi:10.1007/978-1-4684-1233-8_20 · Cited by 39 · see citing works
Effectiveness of morantel tartrate and naphthalophos against levamisole resistantOstertagia in sheep
Jambre, L. F. L., & Martin, P. J. (1979) · Veterinary Research Communications · doi:10.1007/bf02268962 · Cited by 28 · see citing works
Várady, M. (1996) · International Journal for Parasitology · doi:10.1016/0020-7519(96)00007-0 · Cited by 27 · see citing works
Self-citationWaller, P. J., Dobson, R. J., Obendorf, D. L., & Gillham, R. J. (1986) · Veterinary Parasitology · doi:10.1016/0304-4017(86)90051-8 · Cited by 24 · see citing works
Levamisole resistance in Haemonchus contortus selected at different stages of infection
Sangster, N. C., & Bjørn, H. (1995) · International Journal for Parasitology · doi:10.1016/0020-7519(94)e0068-x · Cited by 22 · see citing works
Self-citationGrimshaw, W., Hunt, K. R., Hong, C., & Coles, G. C. (1994) · Veterinary Record · doi:10.1136/vr.135.16.372 · Cited by 22 · see citing works
Anthelmintic resistance in nematodes causing parasitic gastroenteritis of sheep in the UK
HAZELBY, C. A., Probert, A. J., & Rowlands, D. A. T. (1994) · Journal of Veterinary Pharmacology and Therapeutics · doi:10.1111/j.1365-2885.1994.tb00240.x · Cited by 18 · see citing works
McKenna, P. B. (1985) · New Zealand Veterinary Journal · doi:10.1080/00480169.1985.35234 · Cited by 11 · see citing works
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von Samson-Himmelstjerna, G., Coles, G. C., Jackson, F., Bauer, C., Borgsteede, F., Cirak, V. Y., Demeler, J., Donnan, A., Dorny, P., Epe, C., et al. (2009) · Parasitology Research · doi:10.1007/s00436-009-1466-1 · Cited by 141 · see citing works
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Self-citation
- Authors
- Várady, M., Bjørn, H., & Nansen, P.
- Affiliations
- Institute of Parasitology of the Slovak Academy of Sciences (SK)
- Corresponding author
- Nansen, Peter
- ORCID
- Várady, Marián
- Venue
- International Journal for Parasitology
- Publisher
- Elsevier BV
- Published
- 1996-07
- Volume
- 26
- Issue
- 7
- Pages
- 733-740
- ISSN
- 0020-7519 (ISSN-L)
- OpenAlex
- W2043985202
- Cited by
- 31
- References
- 29
- Citations by year
- 2025: 4 · 2023: 1 · 2021: 3 · 2020: 2 · 2019: 4 · 2018: 2 · 2017: 1 · 2016: 1 · 2015: 1 · 2012: 1
- FWCI
- 3.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal for Parasitology (publishedVersion)
- PubMed (publishedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
- Topics
- Health Sciences › Veterinary › Small Animals › Helminth infection and control
- Health Sciences › Veterinary › Small Animals › Veterinary medicine and infectious diseases
- Life Sciences › Immunology and Microbiology › Parasitology › Parasites and Host Interactions
- Keywords
- Oesophagostomum, anthelmintic resistance, Oesophagostomum dentatum, pyrantel, morantel, levamisole, larval development assay, drug susceptibility testing, field isolates
- MeSH terms
- Animals, Antinematodal Agents, Drug Resistance, Ivermectin, Larva, Levamisole, Morantel, Oesophagostomum, Ovum, Pyrantel, Swine, Thiabendazole
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