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Bacteriophage P1 cre gene and its regulatory region
Abstract
The bacteriophage P1 site-specific recombination system consists of two components, a site, loxP, at which recombination occurs, and a recombinase protein, Cre. In this paper, we present the DNA sequence of the cre structural gene and its upstream regulatory region. Analysis of the sequence indicates: (1) that cre encodes a protein of 343 amino acids; (2) that cre and loxP are separated by a 434 base-pair region that contains a 73 amino acid open reading frame, orf1; and (3) that cre and orf1 are oriented with their amino-terminal ends proximal to loxP. We have identified three promoters that are located upstream of the cre structural gene. Their activities range from 7 to 10% of the activity of the galactose operon promoter. The promoter furthest from cre, pR1, contains two Dam methylation sites (5'-G-A-T-C-3') in its -35 region, and is sensitive to Dam methylation. Its transcription is three- to fourfold higher in a dam- host than it is in a dam+ host. The promoter closest to cre, pR3, signals the production of an RNA transcript that functions inefficiently for Cre protein synthesis because it lacks a ribosome recognition site. None of the three cre promoters is sensitive to proteins expressed by the P1 prophage, including the c1 repressor protein. To assess the role of cre in the P1 life-cycle, we isolated cre mutants and studied their behavior in recA+ and recA- hosts. Those studies indicate that Cre is dispensable for viral vegetative growth and lysogeny in a recA+ host,…
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“Microglia are known to play a vital role during typical development in shaping neuronal circuitry by pruning synapses2,11,12.”
From Constitutive expression of CX3CR1-BAC-Cre introduces minimal off-target effects in microglia (2026) · cites this paper as [2]
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“However, alterations in microglial morphology alone cannot predict a change in function2.”
From Constitutive expression of CX3CR1-BAC-Cre introduces minimal off-target effects in microglia (2026) · cites this paper as [2]
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Metadata
- Authors
- Sternberg, N., Sauer, B., Hoess, R., & Abremski, K.
- Affiliations
- Frederick National Laboratory for Cancer Research (US)
- Venue
- Journal of Molecular Biology
- Publisher
- Elsevier BV
- Published
- 1986-01
- Volume
- 187
- Issue
- 2
- Pages
- 197-212
- ISSN
- 0022-2836 (ISSN-L)
- OpenAlex
- W1594256225
- Cited by
- 171
- References
- 36
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 2 · 2023: 5 · 2022: 3 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 4 · 2015: 2 · 2014: 3 · 2013: 7 · 2012: 4
- FWCI
- 20.35
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Molecular Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (n01co023909-011)
- National Cancer Institute (N01-CO23909)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › Bacteriophages and microbial interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Keywords
- bacteriophage P1, Cre recombinase, promoter, Dam methylation, RecA, lysogeny, vegetative growth, viral life cycle
- MeSH terms
- Base Sequence, Coliphages, DNA Nucleotidyltransferases, DNA, Viral, Gene Expression Regulation, Genes, Genes, Regulator, Genes, Viral, Lysogeny, Methylation, Mutation, Plasmids, Promoter Regions, Genetic, Suppression, Genetic, Transcription, Genetic, Protein Biosynthesis, Viral Proteins, Integrases
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