Antagonism between estrogens and androgens on GCDFP-15 gene expression in ZR-75-1 cells and correlation between GCDFP-15 and estrogen as well as progesterone receptor expression in human breast cancer.
Abstract
The androgen dihydrotestosterone (DHT) caused a maximal 65% inhibition of proliferation of the human breast cancer cells ZR-75-1 after a 10-day incubation period. The same treatment, on the other hand, stimulated by 25-fold the secretion of the breast marker protein GCDFP-15 (gross cystic disease fluid protein-15). The stimulatory effect of DHT on GCDFP-15 mRNA accumulation was already significant (1.6-fold, P less than 0.01) after a 12 h exposure and reached a maximal 25-fold increase after a 12-day incubation period. On the other hand, a 2-day exposure to 1 nM 17 beta-estradiol (E2) alone decreased by 60% GCDFP-15 mRNA levels while it completely blocked the 2.5-fold stimulation of GCDFP-15 secretion induced by concomitant incubation with DHT. Furthermore, a 10-day incubation with E2 increased by 4-fold the proliferation of ZR-75-1 cells whereas such treatment decreased by about 85% both GCDFP-15 mRNA accumulation and the secretion of the glycoprotein. The presence of GCDFP-15 mRNA in human breast cancer samples was restricted to estrogen receptor positive tumors and was significantly correlated with progesterone receptor expression.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Our previous studies have clearly demonstrated that androgens exert a direct inhibitory effect on the proliferation of human breast cancer cells.
“our previous studies have clearly demonstrated that androgens exert a direct inhibitory effect on the proliferation of human breast cancer cells (208 -213).”
From Endocrine and intracrine sources of androgens in women: inhibition of breast cancer and other roles of androgens and their precursor dehydroepiandrosterone (2003) · cites this paper as [212]
Cites
Current protocols in molecular biology
Sargent, T. (1988) · Analytical Biochemistry · doi:10.1016/0003-2697(88)90147-9 · Cited by 7,298 · see citing works
Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A
Green, S., Walter, P., Kumar, V., Krust, A., Bornert, J., Argos, P., & Chambon, P. (1986) · Nature · doi:10.1038/320134a0 · Cited by 2,320 · see citing works
Haagenson, C. (1971) · Cited by 1,767 · see citing works
Steroid receptors and other prognostic factors in primary breast cancer.
Clark, G. M., & McGuire, W. L. (1988) · PubMed · Cited by 227 · see citing works
Poulin, R., & Labrie, F. (1986) · PubMed · Cited by 200 · see citing works
Self-citationMurphy, G. P., Beckley, S. A., Brady, M. F., Chu, T. M., deKernion, J. B., Dhabuwala, C. B., Gaeta, J. F., Gibbons, R. P., Loening, S. A., McKiel, C. F., et al. (1983) · Cancer · doi:10.1002/1097-0142(19830401)51:7 · Cited by 173 · see citing works
Poulin, R., Baker, D., & Labrie, F. (1988) · Breast Cancer Research and Treatment · doi:10.1007/bf01805942 · Cited by 172 · see citing works
Self-citationAllegra, J. C., Lippman, M. E., Thompson, E. B., Simon, R., Barlock, A., Green, L., Kk, H., Do, H. M., & Aitken, S. C. (1979) · PubMed · Cited by 162 · see citing works
Cell Proliferation of Estrogen-Sensitive Cells: The Case for Negative Control*
Soto, A. M., & Sonnenschein, C. (1987) · Endocrine Reviews · doi:10.1210/edrv-8-1-44 · Cited by 153 · see citing works
Evaluation of tamoxifen doses with and without fluoxymesterone in advanced breast cancer.
TORMEY, D. C., LIPPMAN, M. E., EDWARDS, B. K., & CASSIDY, J. G. (1983) · Annals of Internal Medicine · doi:10.7326/0003-4819-98-2-139 · Cited by 128 · see citing works
Murphy, L. C., Tsuyuki, D., Myal, Y., et al. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)48164-1 · Cited by 115 · see citing works
Down-regulation of estrogen receptors by androgens in the ZR-75-1 human breast cancer cell line.
POULIN, R., SIMARD, J., LABRIE, C., PETITCLERC, L., DUMONT, M., LAGACÉ, L., & LABRIE, F. (1989) · Endocrinology · doi:10.1210/endo-125-1-392 · Cited by 115 · see citing works
Self-citationGarcia, T., Lehrer, S. P., Bloomer, W. D., et al. (1988) · Molecular Endocrinology · doi:10.1210/mend-2-9-785 · Cited by 107 · see citing works
Self-citationPoulin, R., Baker, D., Poirier, D., & Labrie, F. (1989) · Breast Cancer Research and Treatment · doi:10.1007/bf01806528 · Cited by 83 · see citing works
Self-citationGrowth regulatory peptide production by human breast carcinoma cells
Lippman, M. E., Dickson, R. B., Gelmann, E. P., et al. (1988) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(88)90076-3 · Cited by 73 · see citing works
Simard, J., Hatton, A. C., Labrief, C., Dauvois, S., Zhao, H. F., Haagensen, D. E., & Labrie, F. (1989) · Molecular Endocrinology · doi:10.1210/mend-3-4-694 · Cited by 72 · see citing works
Self-citationMurphy, L. C., Lee-Wing, M. W., Goldenberg, G. J., et al. (1987) · PubMed · Cited by 68 · see citing works
Identification and androgen regulation of two proteins released by T47D human breast cancer cells.
Chalbos, D., Haagensen, D. E., Parish, T., & Rochefort, H. (1987) · PubMed · Cited by 66 · see citing works
Miller, W. R., Telford, J., Dixon, J. M., & Hawkins, R. A. (1985) · European Journal of Cancer and Clinical Oncology · doi:10.1016/0277-5379(85)90049-5 · Cited by 46 · see citing works
Breast gross cystic disease protein 15 in human breast cancer in culture
Miller, W. R., Shivas, A. A., Franchimont, P., et al. (1988) · European Journal of Cancer and Clinical Oncology · doi:10.1016/0277-5379(88)90257-x · Cited by 32 · see citing works
Ingle, J. N., Twito, D. I., Schaid, D. J., et al. (1988) · Journal of Clinical Oncology · doi:10.1200/jco.1988.6.5.825 · Cited by 29 · see citing works
Advances in gene technology: Molecular biology of the endocrine system
McEwen, B. S. (1987) · Psychoneuroendocrinology · doi:10.1016/0306-4530(87)90012-6 · Cited by 26 · see citing works
Fluoxymesterone stimulation of tumor marker secretion in patients with breast carcinoma
Dilley, W. G., Haagensen, D. E., Leight, G. S., AMMIRATA, S., Davis, S. R., Silva, J. S., Zamcheck, N., Lokich, J. J., & Wells, S. A. (1986) · Breast Cancer Research and Treatment · doi:10.1007/bf01807333 · Cited by 17 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Labrie, F., Luu-The, V., Labrie, C., Bélanger, A., Simard, J., Lin, S.-X., & Pelletier, G. (2003) · Endocrine Reviews · doi:10.1210/er.2001-0031 · Cited by 544 · see citing works
Self-citationPhenotypic and functional characterisation of the luminal cell hierarchy of the mammary gland
Shehata, M., Teschendorff, A., Sharp, G., Novcic, N., Russell, I. A., Avril, S., Prater, M., Eirew, P., Caldas, C., Watson, C. J., et al. (2012) · Breast Cancer Research · doi:10.1186/bcr3334 · Cited by 306 · see citing works
SIMARD, J., DAUVOIS, S., HAAGENSEN, D. E., LÉVESQUE, C., MÉRAND, Y., & LABRIE, F. (1990) · Endocrinology · doi:10.1210/endo-126-6-3223 · Cited by 107 · see citing works
Self-citationCouillard, S., Labrie, C., Bélanger, A., Candas, B., Pouliot, F., & Labrie, F. (1998) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/90.10.772 · Cited by 38 · see citing works
Self-citationLabrie, F., Li, S., B�langer, A., C�t�, J., M�rand, Y., & Lepage, M. (1993) · Breast Cancer Research and Treatment · doi:10.1007/bf00665803 · Cited by 18 · see citing works
Self-citation
- Authors
- Dumont, M., Dauvois, S., Simard, J., Garcia, T., Schachter, B., & Labrie, F.
- Affiliations
- Université Laval (CA)
- Icahn School of Medicine at Mount Sinai (US)
- Venue
- Journal of Steroid Biochemistry
- Publisher
- Elsevier BV
- Published
- 1989
- Volume
- 34
- Issue
- 1-6
- Pages
- 397-402
- ISSN
- 0022-4731 (ISSN-L)
- OpenAlex
- W2036754450
- Cited by
- 26
- References
- 23
- Citations by year
- 2023: 1 · 2022: 1 · 2021: 2 · 2018: 1 · 2016: 1 · 2012: 1
- FWCI
- 0.88
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Steroid Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Fonds pour la Formation de Chercheurs et l'Aide à la Recherche
- Medical Research Council Canada
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Breast Cancer Treatment Studies
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Prostate Cancer Treatment and Research
- Keywords
- GCDFP-15, estrogen receptor, progesterone receptor, androgen receptor, dihydrotestosterone, estradiol, breast cancer, ZR-75-1 cells, cell proliferation, gene expression regulation
- MeSH terms
- Apolipoproteins, Breast Neoplasms, Carrier Proteins, Cell Line, Estradiol, Female, Glycoproteins, Humans, Kinetics, Neoplasm Proteins, RNA, Messenger, Dihydrotestosterone, Transcription, Genetic, Tumor Cells, Cultured, Biomarkers, Tumor, Gene Expression, Membrane Transport Proteins, Apolipoproteins D
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
