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Possible role of regional superoxide dismutase activity and lipid peroxide levels in cadmium neurotoxicity: in vivo and in vitro studies in growing rats
Abstract
Cd2+ (0.4 mg/kg) administration to growing rats (45 +/- 5 g) intraperitoneally, daily for 30 days was found to decrease the activity of superoxide dismutase (SOD) in all the brain regions, except hippocampus. The concentrations of lipid peroxides were significantly elevated in the cerebellum, cerebral cortex, corpus striatum and midbrain. A 100% inhibition in SOD activity was observed by 14 microM and 50 microM of Cd2+ in bovine blood and rat brain preparations, respectively. Cadmium-induced strong inhibitory effect on brain and purified bovine blood SOD suggested a direct effect of the metal on enzyme molecule. Furthermore, in vitro addition of a wide range of Cd2+ (1-100 microM) increased the rate of lipid peroxidation (LPO) reaction in fresh brain homogenate, however, did not affect boiled homogenate. The studies on LPO in reconstituted homogenate resulting from mixing of fresh and/or heated different subcellular fractions indicated the presence of some heat-labile Cd2+ -sensitive factor in 15000 x g pellet fraction. It is suggested that Cd2+ directly and indirectly through inhibition of SOD, increases the LPO of cell membranes and thus produces damage to the associated physiological functions leading to central nervous dysfunctions.
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“while also helping antioxidant defense mechanisms through the management of GST [260].”
From Oxidative Stress Implications in the Affective Disorders: Main Biomarkers, Animal Models Relevance, Genetic Perspectives, and Antioxidant Approaches (2016) · cites this paper as [260]
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Self-citation
Metadata
- Authors
- Shukla, G. S., Hussain, T., & Chandra, S. V.
- Affiliations
- Indian Institute of Toxicology Research (IN)
- Venue
- Life Sciences
- Publisher
- Elsevier BV
- Published
- 1987-11
- Volume
- 41
- Issue
- 19
- Pages
- 2215-2221
- ISSN
- 0024-3205 (ISSN-L)
- OpenAlex
- W2017924999
- Cited by
- 113
- References
- 42
- Citations by year
- 2023: 1 · 2022: 2 · 2021: 1 · 2020: 2 · 2019: 2 · 2018: 4 · 2017: 3 · 2016: 3 · 2015: 1 · 2014: 1 · 2013: 5 · 2012: 2
- FWCI
- 2.38
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Life Sciences (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
- Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
- Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
- Keywords
- cadmium neurotoxicity, superoxide dismutase inhibition, lipid peroxidation, lipid hydroperoxides, brain, cadmium, oxidative stress, heat-labile factor, subcellular fractionation, growing rats
- MeSH terms
- Animals, Brain, Cadmium, Hot Temperature, Hippocampus, Lipid Peroxides, Male, Superoxide Dismutase, Rats, In Vitro Techniques
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