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Cited by 130Sexual Differentiation and DisordersEstrogen and related hormone effectsNeuroendocrine regulation and behavior

Immunohistochemical labeling of androgen receptors in the brain of rat and monkey

Clancy, Andrew N., Bonsall, Robert W., Michael, Richard P.·1992·Life Sciences
DOI 10.1016/0024-3205(92)90375-y
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Abstract

Androgen receptor antibodies have recently been developed using fusion proteins containing fragments of human prostatic androgen receptor. We have used a polyclonal antibody raised in rabbits to label androgen receptors in brain sections from male and female rats and monkeys. Free-floating frozen sections were incubated in primary antibody, and processed by the peroxidase-avidin-biotin complex method using biotinylated anti-rabbit IgG. Nickel intensified diaminobenzidine was used as the chromagen, and neurons were labeled in the amygdala, hippocampus, bed nucleus of stria terminalis, septum, preoptic area, in several hypothalamic nuclei including the supraoptic and paraventricular nuclei, in several brain stem motor nuclei and in cerebral cortex. Staining was most intense in cell nuclei but also occurred in cytoplasm and in some neuronal processes. Labeling was more restricted in monkey than in rat brain. Omitting the primary antibody or pre-incubating the primary antibody with rat prostatic cytosol for control purposes demonstrated the specificity of staining.

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Crossref · 10.1016/0024-3205(92)90375-y

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  • In adults, AR is expressed across the cerebral cortex, including associative cortex, in both sexes in rodents and humans.

    “In adults AR is expressed across the cerebral cortex, including associative cortex, in both sexes in rodents (Clancy et al., 1992; Dart et al., 2013; DonCarlos et al., 2006; Feng et al., 2010; Kritzer, 2004; Simerly et al., 1990; Young and Chang, 1998), and humans (Bezdickova et al., 2007; Puy et al., 1995; Sarrieau et al., 1990).”

    From Does puberty mark a transition in sensitive periods for plasticity in the associative neocortex? (2017) · cites this paper as Clancy et al., 1992

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Authors
Clancy, A. N., Bonsall, R. W., & Michael, R. P.
Affiliations
Emory University (US)
Corresponding author
Michael, Richard P.
Venue
Life Sciences
Publisher
Elsevier BV
Published
1992-01
Volume
50
Issue
6
Pages
409-417
ISSN
0024-3205 (ISSN-L)
DOI
10.1016/0024-3205(92)90375-y
OpenAlex
W1998072745
Cited by
130
References
19
Citations by year
2025: 1 · 2024: 1 · 2023: 4 · 2022: 3 · 2021: 4 · 2019: 1 · 2018: 2 · 2017: 3 · 2016: 3 · 2015: 2 · 2014: 3 · 2013: 3 · 2012: 8
FWCI
2.51
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Life Sciences (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01mh019506-24, 5r01mh040420-04)
  • National Institute of Mental Health (MH-40420, MH19506)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sexual Differentiation and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
  • Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
Keywords
androgen receptor, brain, immunohistochemistry, polyclonal antibodies, rats, monkeys, amygdala, hippocampus, bed nucleus of the stria terminalis, septum, preoptic area, hypothalamus, cerebral cortex, receptor localization, species differences
MeSH terms
Animals, Brain, Brain Chemistry, Female, Immunohistochemistry, Macaca fascicularis, Macaca mulatta, Male, Neurons, Rats, Inbred Strains, Receptors, Androgen, Dihydrotestosterone, Testosterone, Rats

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