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Cannabinoid receptors in rat brain areas: sexual differences, fluctuations during estrous cycle and changes after gonadectomy and sex steroid replacement.
Abstract
Cannabinoid effects on brain dopaminergic activity vary as a function of gonadal status. In this work, we examined whether these variations might be due to sex steroid-dependent differences in brain cannabinoid receptors (CNr). Four experiments were done: (i) male versus females; (ii) females at each stage of the ovarian cycle; (iii) estradiol (E2) and/or progesterone (P)-replaced ovariectomized (OVX) females; and (iv) testosterone (T)-replaced orchidectomized males. The density of CNr in the medial basal hypothalamus fluctuated in females during the estrous cycle. The density was higher in diestrus and decreased in estrus. This parameter did not change after ovariectomy and E2 replacement. However, P increased the density of CNr when administered to OVX rats acutely treated with E2, but not administered alone or after chronic E2 treatment. In the striatum, the affinity of CNr was slightly higher in males than females, with no changes in density. Ovariectomy increased the affinity of CNr, which normalized only after administration of acute E2. Interestingly, the high affinity values observed in this area after P alone or combined with E2, corresponded to low densities as compared with intact females. In the limbic forebrain, the affinity for the cannabinoid ligand was also higher in males than females with no changes in density. Affinity was also higher in diestrus and lower in estrus, whereas density was unchanged. Ovariectomy decreased CNr density. A normal situation was found after administration of acute E2 or P alone, whereas chronic E2 markedly increased the density of CNr as compared with both intact and OVX females. Interestingly, this latter increase was prevented by coadministration of P. Orchidectomy did not affect CNr density, but administration of T produced a marked decrease. In the mesencephalon, the density and affinity of CNr was higher in males than females. Administration of P to OVX rats produced opposite effects, increasing the density when administered alone and decreasing it when administered to acute E2-replaced OVX rats. In summary, these results reveal the existence of subtle, sometimes more pronounced, sex dimorphisms, fluctuations along the ovarian cycle and changes after gonadectomy and sex steroid replacement in CNr density and affinity in certain brain areas. This supports the hypothesis of possible sex steroid-dependent differences in the sensitivity of certain neuronal processes to cannabinoid treatment.
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“both studies observed ovarian cyclical changes in cannabinoid receptor density or endocannabinoid content in brain areas potentially associated with nociception, [8,44].”
From Endocannabinoid involvement in endometriosis. (2010) · cites this paper as [44]
Citations
Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 316,394
Isolation and Structure of a Brain Constituent That Binds to the Cannabinoid Receptor
Devane, W. A., Hanuš, L., Breuer, A., Pertwee, R. G., Stevenson, L. A., Griffin, G., Gibson, D., Mandelbaum, A., Etinger, A., & Mechoulam, R. (1992) · Science · doi:10.1126/science.1470919 · Cited by 5,633
REGIONAL STUDIES OF CATECHOLAMINES IN THE RAT BRAIN‐I
Glowinski, J., & Iversen, L. L. (1966) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1966.tb09873.x · Cited by 5,436
Structure of a cannabinoid receptor and functional expression of the cloned cDNA
Matsuda, L. A., Lolait, S. J., Brownstein, M. J., Young, A. C., & Bonner, T. I. (1990) · Nature · doi:10.1038/346561a0 · Cited by 5,011
Determination and characterization of a cannabinoid receptor in rat brain
Devane, W. A., Dysarz, F. A., Johnson, M. R., Melvin, L. S., & Howlett, A. C. (1988) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)09876-1 · Cited by 2,443
Herkenham, M., Lynn, A., Johnson, M., Melvin, L., de Costa, B., & Rice, K. (1991) · The Journal of Neuroscience · doi:10.1523/jneurosci.11-02-00563.1991 · Cited by 2,159
Neuronal localization of cannabinoid receptors in the basal ganglia of the rat
Herkenham, M. A., Lynn, A. B., de Costa, B. R., & Richfield, E. K. (1991) · Brain Research · doi:10.1016/0006-8993(91)90970-7 · Cited by 515
Involvement of Gi in the inhibition of adenylate cyclase by cannabimimetic drugs.
Howlett, A. C., Qualy, J. M., & Khachatrian, L. (1986) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)10219-8 · Cited by 470
Chen, J., Paredes, W. M., Li, J., Smith, D. M., Lowinson, J. H., & Gardner, E. L. (1990) · Psychopharmacology · doi:10.1007/bf02245916 · Cited by 329
Howlett, A. C., Johnson, M. R., Melvin, L. S., & Milne, G. M. (1988) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)13196-9 · Cited by 253
Cannabinoid Receptors and Modulation of Cyclic AMP Accumulation in the Rat Brain
Bidaut‐Russell, M., Devane, W. A., & Howlett, A. C. (1990) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1990.tb08815.x · Cited by 208
Facilitation of brain stimulation reward byΔ 9-tetrahydrocannabinol
Gardner, E. L., Paredes, W. M., Smith, D. M., Donner, A., Milling, C., Cohen, D., & Morrison, D. (1988) · Psychopharmacology · doi:10.1007/bf02431546 · Cited by 204
Biochemistry and Physiology of Substance Abuse
WATSON, R. R. (1990) · Medical Entomology and Zoology · Cited by 202
17β-estradiol at a physiological dose acutely increases dopamine turnover in rat brain
Paolo, T. D., Rouillard, C., & Bédard, P. J. (1985) · European Journal of Pharmacology · doi:10.1016/0014-2999(85)90604-1 · Cited by 195
The Cannabinoids: Chemical, Pharmacologic, and Therapeutic Aspects
(1984) · Elsevier eBooks · doi:10.1016/b978-0-12-044620-9.x5001-5 · Cited by 189
de Fonseca, F. R., Cebeira, M., Fernández‐Ruíz, J., Navarro, M. A., & Ramos, J. A. (1991) · Neuroscience · doi:10.1016/0306-4522(91)90329-m · Cited by 126
Self-citationStriatal D1 dopamine receptor density fluctuates during the rat estrous cycle
Lévesque, D., Gagnon, S., & Paolo, T. D. (1989) · Neuroscience Letters · doi:10.1016/0304-3940(89)90426-6 · Cited by 89
A physiological dose of progesterone affects rat striatum biogenic amine metabolism
Paolo, T. D., Lévesque, D., & Daigle, M. (1986) · European Journal of Pharmacology · doi:10.1016/0014-2999(86)90077-4 · Cited by 74
Regulation of adenylate cyclase by chronic exposure to cannabimimetic drugs.
Dill, J. A., & Howlett, A. C. (1988) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)24581-5 · Cited by 73
Fernández‐Ruíz, J., Amor, J. C., & Ramos, J. A. (1989) · Brain Research · doi:10.1016/0006-8993(89)91266-3 · Cited by 70
Self-citationAcute effects of δ-9-tetrahydrocannabinol on dopaminergic activity in several rat brain areas
de Fonseca, F. R., Fernández‐Ruíz, J., Murphy, L. L., Cebeira, M., Steger, R. W., Artke, J. A., & Ramos, J. A. (1992) · Pharmacology Biochemistry and Behavior · doi:10.1016/0091-3057(92)90526-l · Cited by 69
Self-citationFernández‐Ruíz, J., de Miguel, R., Hernández, M., & Ramos, J. A. (1990) · Pharmacology Biochemistry and Behavior · doi:10.1016/0091-3057(90)90262-g · Cited by 41
Self-citationde Fonseca, F. R., Hernández, M., de Miguel, R., Fernández‐Ruíz, J., & Ramos, J. A. (1992) · Pharmacology Biochemistry and Behavior · doi:10.1016/0091-3057(92)90359-n · Cited by 37
Self-citationChanges in brain dopaminergic indices induced by perinatal exposure to cannabinoids in rats
de Fonseca, F. R., Cebeira, M., Hernández, M., Ramos, J. A., & Fernández‐Ruíz, J. (1990) · Developmental Brain Research · doi:10.1016/0165-3806(90)90280-c · Cited by 34
Self-citationEvidence for a direct anterior pituitary site of delta-9-tetrahydrocannabinol action
Murphy, L. L., Newton, S. C., Dhali, J., & Chávez, D. (1991) · Pharmacology Biochemistry and Behavior · doi:10.1016/0091-3057(91)90370-h · Cited by 31
Murphy, L. L., de Fonseca, F. R., & Steger, R. W. (1991) · Steroids · doi:10.1016/0039-128x(91)90131-e · Cited by 24
Role of estrogens on striatal dopaminergic activity
Ramos, J. A., Pais, J., Cebeira, M., & Fernández‐Ruíz, J. (1987) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(87)90137-3 · Cited by 20
Self-citationInfluence of Estradiol and Progesterone on Pulsatile LH Secretion in 8-Day Ovariectomized Rats
Leipheimer, R. E., Bona‐Gallo, A., & Gallo, R. V. (2008) · Neuroendocrinology · doi:10.1159/000124544 · Cited by 15
Bonnin, A., Fern�ndez-Ruiz, J. J., Martı́n, M. I., de Fonseca, F. R., de Miguel, R., & Ramos, J. A. (1992) · Molecular and Cellular Neuroscience · doi:10.1016/1044-7431(92)90028-z · Cited by 11
Self-citation
Research works printed but not linked: 13
- Dill, 1988, J. Pharmacol. Exp. Ther., 244, 1157
- Howlett, 1990, TINS, 13, 420
- Devane, 1988, Mol. Pharmacol., 34, 605
- Herkenham, 1990, 87, 1932
- Howlett, 1986, Mol. Pharmacol., 29, 307
- Dewey, 1986, Pharmacol. Rev., 38, 151
- Howlett, 1988, Mol. Pharmacol., 33, 297
- Pertwee, 1988, Neuropharmacology, 27, 1256
- Bonnin, 1993, Neuroendocrinology
- Gunnet, 1986, 183, 48
- Bridgen, 1990, FASEB J., A996, 4240
- Houston, 1991, Du Pont Biotech Update, 6, 21
- de Fonseca, 1993, Pharmacol. Biochem. Behav.
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Pagotto, U., Marsicano, G., Cota, D., Lutz, B., & Pasquali, R. (2005) · Endocrine Reviews · doi:10.1210/er.2005-0009 · Cited by 891
Neumeister, A., Normandin, M. D., Pietrzak, R. H., Piomelli, D., Zheng, M. Q., Gujarro-Anton, A., Potenza, M. N., Bailey, C. R., Lin, S. F., Najafzadeh, S., et al. (2013) · Molecular Psychiatry · doi:10.1038/mp.2013.61 · Cited by 361
Sex-Dependent Effects of Cannabis and Cannabinoids: A Translational Perspective
Cooper, Z. D., & Craft, R. M. (2017) · Neuropsychopharmacology · doi:10.1038/npp.2017.140 · Cited by 369
Puberty as a highly vulnerable developmental period for the consequences of cannabis exposure
Schneider, M. (2008) · Addiction Biology · doi:10.1111/j.1369-1600.2008.00110.x · Cited by 330
Bisogno, T., Berrendero, F., Ambrosino, G., Cebeira, M., Ramos, J. A., Fernández‐Ruíz, J., & Marzo, V. D. (1999) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1999.0254 · Cited by 313
Self-citation
Metadata
- Authors
- de Fonseca, F. R., Cebeira, M., Ramos, J. A., Martín, M., & Fernández-Ruiz, J. J.
- Affiliations
- Universidad Complutense de Madrid (ES)
- ORCID
- Martín, M.
- Venue
- Life Sciences
- Publisher
- Elsevier BV
- Published
- 1994-01
- Volume
- 54
- Issue
- 3
- Pages
- 159-170
- ISSN
- 0024-3205 (ISSN-L)
- OpenAlex
- W2056795643
- Cited by
- 243
- References
- 29
- Citations by year
- 2026: 6 · 2025: 9 · 2024: 11 · 2023: 18 · 2022: 12 · 2021: 18 · 2020: 14 · 2019: 4 · 2018: 10 · 2017: 12 · 2016: 8 · 2015: 11 · 2014: 9 · 2013: 9 · 2012: 9
- FWCI
- 2.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Life Sciences (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Pharmacology (medical) › Cannabis and Cannabinoid Research
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
- Keywords
- cannabinoid receptors, sex differences, estrous cycle, gonadectomy, estradiol, progesterone, testosterone, ovariectomy, orchiectomy, receptor density, receptor binding affinity, medial basal hypothalamus, striatum, limbic forebrain, midbrain, dopaminergic activity, gonadal status
- MeSH terms
- Animals, Brain, Cannabinoids, Cyclohexanols, Dopamine, Estradiol, Estrus, Female, Kinetics, Male, Neurons, Orchiectomy, Ovariectomy, Ovary, Progesterone, Receptors, Drug, Sex Characteristics, Testis, Testosterone, Dronabinol, Tritium, Rats, Wistar, Receptors, Cannabinoid, Rats
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