Estrogenic regulation of limbic cannabinoid receptor binding
Open accessAbstract
Sex differences have been identified in many of the behavioral and physiological effects of cannabinoids. While estrogen has been linked to some of these variations, the effects of estrogen on cannabinoid receptor binding have not been characterized within regions of the brain specifically implicated in stress responsivity and emotional behavior. To examine sex differences, and the role of estradiol, in regulation of the cannabinoid receptor, we compared the binding site density of the cannabinoid receptor within the amygdala, hippocampus and hypothalamus in males, cycling females, ovariectomized (OVX) females and estradiol-treated OVX females (OVX+E). Our data reveal that males and OVX females have higher amounts of hypothalamic and lower amounts of amygdalar cannabinoid receptor binding relative to both cycling females and OVX+E females. Within the hippocampus, ovariectomy resulted in an upregulation of cannabinoid receptor binding. These data provide a putative biochemical mechanism mediating the observed behavioral and physiological sex differences in the effects of cannabinoids, particularly with respect to stress and emotional behavior.
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In animals, CB1 receptor levels are higher in males than females.
“Animal data showing higher CB1 receptor levels in male relative to female animals18, 56, 57”
From Elevated brain cannabinoid CB1 receptor availability in post-traumatic stress disorder: A positron emission tomography study (2013) · cites this paper as [56]
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- Authors
- Riebe, C. J. N., Hill, M. N., Lee, T. T. Y., Hillard, C. J., & Gorzalka, B. B.
- Affiliations
- University of British Columbia (CA)
- Medical College of Wisconsin (US)
- Corresponding author
- Gorzalka, Boris B.
- Venue
- Psychoneuroendocrinology
- Publisher
- Elsevier BV
- Published
- 2010-09
- Volume
- 35
- Issue
- 8
- Pages
- 1265-1269
- ISSN
- 0306-4530 (ISSN-L)
- PMC
- PMC2933663
- OpenAlex
- W2038447112
- Cited by
- 128
- References
- 19
- Citations by year
- 2026: 6 · 2025: 11 · 2024: 3 · 2023: 13 · 2022: 10 · 2021: 14 · 2020: 7 · 2019: 2 · 2018: 8 · 2017: 8 · 2016: 9 · 2015: 11 · 2014: 7 · 2013: 8 · 2012: 5
- FWCI
- 4.48
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Psychoneuroendocrinology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Canadian Institutes of Health Research
- National Institute on Drug Abuse (R01DA009155, DA09155, DA022439, R21DA022439)
- Topics
- Health Sciences › Medicine › Pharmacology › Cannabis and Cannabinoid Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Keywords
- estrogen, estradiol, cannabinoid receptors, sex differences, limbic system, amygdala, hippocampus, hypothalamus, ovariectomy, receptor binding density, stress responsivity, emotional behavior
- MeSH terms
- Amygdala, Animals, Estradiol, Female, Hippocampus, Hypothalamus, Limbic System, Male, Ovariectomy, Protein Binding, Rats, Sprague-Dawley, Estrous Cycle, Receptors, Cannabinoid, Rats, Cannabinoid Receptor Agonists
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