Cited by 5,633Cannabis and Cannabinoid ResearchFatty Acid Research and HealthNeuroscience and Neuropharmacology Research

Isolation and Structure of a Brain Constituent That Binds to the Cannabinoid Receptor

Devane, William A., Hanuš, Lumir, Breuer, Aviva, Pertwee, Roger G., Stevenson, Lesley A., Griffin, Graeme, Gibson, Dan, Mandelbaum, Asher +2 more·1992·Science

Abstract

Arachidonylethanolamide, an arachidonic acid derivative in porcine brain, was identified in a screen for endogenous ligands for the cannabinoid receptor. The structure of this compound, which has been named "anandamide," was determined by mass spectrometry and nuclear magnetic resonance spectroscopy and was confirmed by synthesis. Anandamide inhibited the specific binding of a radiolabeled cannabinoid probe to synaptosomal membranes in a manner typical of competitive ligands and produced a concentration-dependent inhibition of the electrically evoked twitch response to the mouse vas deferens, a characteristic effect of psychotropic cannabinoids. These properties suggest that anandamide may function as a natural ligand for the cannabinoid receptor.

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Crossref · 10.1126/science.1470919

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Authors
Devane, W. A., Hanuš, L., Breuer, A., Pertwee, R. G., Stevenson, L. A., Griffin, G., Gibson, D., Mandelbaum, A., Etinger, A., & Mechoulam, R.
Affiliations
  • Hebrew University of Jerusalem (IL)
  • University of Aberdeen (GB)
  • Technion – Israel Institute of Technology (IL)
Venue
Science
Published
1992-12-18
Volume
258
Issue
5090
Pages
1946-1949
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W1989073099
Cited by
5,633
References
21
Citations by year
2026: 58 · 2025: 122 · 2024: 137 · 2023: 161 · 2022: 163 · 2021: 232 · 2020: 235 · 2019: 184 · 2018: 166 · 2017: 170 · 2016: 173 · 2015: 161 · 2014: 190 · 2013: 173 · 2012: 232
FWCI
50.65
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Wellcome Trust
  • National Institutes of Health (5r01da006481-03)
  • National Institute on Drug Abuse (DA 6481)
Topics
  • Health Sciences › Medicine › Pharmacology › Cannabis and Cannabinoid Research
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
anandamide, cannabinoid receptors, competitive receptor binding, synaptosomal membranes, vas deferens contraction, porcine brain, structure elucidation
MeSH terms
Amides, Animals, Arachidonic Acids, Binding, Competitive, Brain, Brain Chemistry, Cannabinoids, Chromatography, Thin Layer, Fatty Acids, Unsaturated, Kinetics, Gas Chromatography-Mass Spectrometry, Receptors, Drug, Swine, Receptors, Cannabinoid, Polyunsaturated Alkamides, Endocannabinoids

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