The influence of caffeine on cell survival in excision-proficient and excision-deficient xeroderma pigmentosum and normal human cell strains following ultraviolet-light irradiation
Abstract
A uniform response to UV of four normal cell strains was demonstrated. One excision-proficient xeroderma pigmentosum variant strain (XP7TA) had a wild-type UV response but a second (XP30RO) was more sensitive. An excision-deficient xeroderma pigmentosum strain XP4L0 was substantially more sensitive than wild-type cell strains. A continuous post-irradiation treatment with non-toxic levels of caffeine enhanced the lethal effect of UV light in both xeroderma pigmentosum variant cell strains but not in cells from normal individuals. There was no detectable effect on cells from a xeroderma pigmentosum individual from complementation group A. These results correlate well with observations on the influence of caffeine on post-replication repair in the three classes of cells.
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UVC sensitization by caffeine is a hallmark of the XP-V cell phenotype.
“Because UVC sensitization by caffeine (CAF) is a hallmark of the XP-V cell phenotype [11,22,37]”
From Gap-filling and bypass at the replication fork are both active mechanisms for tolerance of low-dose ultraviolet-induced DNA damage in the human genome (2014) · cites this paper as [37]
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Selsky, C. A., & Greer, S. B. (1978) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/0027-5107(78)90044-1 · Cited by 22 · see citing works
Marshall, R., & Scott, D. (1976) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/0027-5107(76)90251-7 · Cited by 23 · see citing works
A Human Subject with A New Defect in Repair of Ultraviolet Damage
Arlett, C. F., Lehmann, A. R., Giannelli, F., & Ramsay, C. A. (1978) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12541290 · Cited by 23 · see citing works
Self-citationPachydermoperiostosis with cutaneous squamous cell carcinomas
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Petit-Frère, C., Clingen, P. H., Arlett, C. F., & Green, M. H. L. (1996) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/0027-5107(96)00042-5 · Cited by 21 · see citing works
Self-citationThe action of AF 2 on cultured hamster and human cells under aerobic and hypoxic conditions
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Self-citationPolymerase η suppresses telomere defects induced by DNA damaging agents
Pope-Varsalona, H., Liu, F., Guzik, L. J., & Opresko, P. L. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku1030 · Cited by 20 · see citing works
Xeroderma pigmentosum variant cells are resistant to immortalization
Volpe, J. P. G., & Cleaver, J. E. (1995) · Mutation Research/DNA Repair · doi:10.1016/0921-8777(95)00015-c · Cited by 18 · see citing works
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Zampetti‐Bosseler, F., & Scott, D. (1985) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/0027-5107(85)90187-3 · Cited by 19 · see citing works
Age-dependent Changes in Fibroblast Cultures from a Xeroderma Pigmentosum Variant
Fujiwara, Y., & Satoh, Y. (1981) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12525772 · Cited by 19 · see citing works
Cytological characterization of repair-deficient CHO cell line 43-3B
Darroudi, F., & Natarajan, A. T. (1985) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/0027-5107(85)90030-2 · Cited by 19 · see citing works
Johansson, F., Lagerqvist, A., Filippi, S., Palitti, F., Erixon, K., Helleday, T., & Jenssen, D. (2006) · DNA repair · doi:10.1016/j.dnarep.2006.07.005 · Cited by 17 · see citing works
- Authors
- Arlett, C. F., Harcourt, S. A., & Broughton, B. C.
- Affiliations
- University of Sussex (GB)
- Venue
- Mutation Research
- Publisher
- Elsevier BV
- Published
- 1975-12
- Volume
- 33
- Issue
- 2-3
- Pages
- 341-346
- ISSN
- 0027-5107 (ISSN-L)
- OpenAlex
- W2001741635
- Cited by
- 140
- References
- 14
- Citations by year
- 2024: 1 · 2021: 1 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 7 · 2015: 2 · 2014: 3 · 2013: 4 · 2012: 4
- FWCI
- 3.51
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Mutation research. Fundamental and molecular mechanisms of mutagenesis (publishedVersion)
- PubMed (publishedVersion)
- Funders
- H2020 Euratom
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Keywords
- caffeine, xeroderma pigmentosum, ultraviolet radiation, cell survival, excision repair, postreplication repair
- MeSH terms
- Caffeine, Cell Survival, DNA Repair, Fibroblasts, Humans, Radiation Genetics, Ultraviolet Rays, Xeroderma Pigmentosum
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