Gas chromatographic and mass spectrometric analysis of urinary acidic metabolites of cortisol
Abstract
A method is described suitable for the analysis of the urinary acidic metabolites of cortisol which are amongst the major metabolites of this hormone (5-25% of secretion). Following hydrolysis of the urinary glucuronide conjugates and extraction of the freed steroids, methyl ester-trimethylsilyl ethers were prepared for gas chromatographic analysis. This analysis was carried out on open tubular columns coated with Carbowax stationary phase. The polar phase column permitted the complete resolution of the four acidic metabolites: alpha-cortolonic, beta-cortolonic, alpha-cortolic and beta-cortolic acids.
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20α/β-reduction of tetrahydrocortisol (THF) yields cortols (α- and β-cortol), and that of tetrahydrocortisone (THE) yields cortolones (α- and β-cortolone).
“Of quantitative importance is the 20α/β-reduction of tetrahydrocortisol (THF), yielding the so called cortols (α- and β-cortol), and that of tetrahydrocortisone (THE), which yields the equivalent cortolones (α- and β-cortolone) [253].”
From Human steroid biosynthesis, metabolism and excretion are differentially reflected by serum and urine steroid metabolomes: a comprehensive review (2019) · cites this paper as [253]
Cites
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Deactivation of glass capillary columns by silylation. Part 1: Principles and basic technique
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Isolation and Identification of Four New Carboxylie Acid Metabolites of Cortisol in Man
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Schiffer, L., Barnard, L., Baranowski, E. S., Gilligan, L. C., Taylor, A. E., Arlt, W., Shackleton, C. H. L., & Storbeck, K.-H. (2019) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2019.105439 · Cited by 450 · see citing works
“Of quantitative importance is the 20α/β-reduction of tetrahydrocortisol (THF), yielding the so called cortols (α- and β-cortol), and that of tetrahydrocortisone (THE), which yields the equivalent cortolones (α- and β-cortolone) [253].”
Self-citationProfiling steroid hormones and urinary steroids
Shackleton, C. H. L. (1986) · Journal of Chromatography B Biomedical Sciences and Applications · doi:10.1016/s0378-4347(00)80683-0 · Cited by 291 · see citing works
- Authors
- Shackleton, C. H. L., Roitman, E., Monder, C., & Bradlow, H. L.
- Affiliations
- University of California, Berkeley (US)
- Icahn School of Medicine at Mount Sinai (US)
- Rockefeller University (US)
- ORCID
- Bradlow, H.L.
- Venue
- Steroids
- Publisher
- Elsevier BV
- Published
- 1980-09
- Volume
- 36
- Issue
- 3
- Pages
- 289-298
- ISSN
- 0039-128X (ISSN-L)
- OpenAlex
- W2082350866
- Cited by
- 16
- References
- 8
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2019: 2
- FWCI
- 0.83
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Steroids (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Keywords
- cortisol, urinary steroid metabolites, glucuronide conjugates, hydrolysis, polar stationary phases, Carbowax, steroid analysis
- MeSH terms
- Carboxylic Acids, Chromatography, Humans, Hydrocortisone, Gas Chromatography-Mass Spectrometry, Stigmasterol
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