Profiling steroid hormones and urinary steroids
Abstract
This paper reviews techniques utilized in the profiling of steroids in body fluids and tissues. Methods for profiling plasma unconjugated steroids and urinary steroid metabolites are focused on. Concentrations or levels of excretion of a variety of steroids have been documented and reviewed. The importance of profiling techniques in the study of normal and pathophysiology of hormonal steroids is discussed.
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Currently, gas chromatography-mass spectrometry (GC-MS) is the preferred method for the analysis of urine steroids in research laboratories due to the unparalleled resolution offered by this technique.
“Currently, gas chromatography-mass spectrometry (GC-MS) is the preferred method for the analysis of urine steroids in research laboratories due to the unparalleled resolution offered by this technique [339,340].”
From Human steroid biosynthesis, metabolism and excretion are differentially reflected by serum and urine steroid metabolomes: a comprehensive review (2019) · cites this paper as [339]
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- Authors
- Shackleton, C. H. L.
- Corresponding author
- Shackleton, C.H.L.
- Venue
- Journal of Chromatography B Biomedical Sciences and Applications
- Publisher
- Elsevier BV
- Published
- 1986-06
- Volume
- 379
- Pages
- 91-156
- ISSN
- 0378-4347 (ISSN-L)
- OpenAlex
- W2001707407
- Cited by
- 291
- References
- 204
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 12 · 2022: 6 · 2021: 5 · 2020: 5 · 2019: 10 · 2018: 11 · 2017: 11 · 2016: 4 · 2015: 5 · 2014: 11 · 2013: 14 · 2012: 11
- FWCI
- 3.23
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Chromatography B Biomedical Sciences and Applications (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01am034400-02)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Keywords
- steroid hormones, urinary steroid metabolites, steroid profiling, steroid excretion
- MeSH terms
- Animals, Bile, Body Fluids, Chromatography, Gas, Chromatography, High Pressure Liquid, Chromatography, Liquid, Chromatography, Paper, Chromatography, Thin Layer, Estrogens, Feces, Hormones, Humans, Hydrolysis, Gas Chromatography-Mass Spectrometry, Steroids
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