The DOI matches its Crossref record; 40 of the 44 references it cites were found in a registry or an index.
The transposon Tn9 generates a 9 bp repeated sequence during integration
Abstract
We performed a genetic and sequencing analysis of insertions of the transposon Tn9 into the lac operon of E. coli. Genetic mapping of 70 insertions into lacl and Z shows that starting from the same point on the chromosome, Tn9 goes to at least 50 different points in these two genes. Although there are preferred regions for insertion, these consist of multiple integration points within a small area, as demonstrated by pairwise crosses and restriction mapping. Sequence analysis of three Tn9 insertions reveals that Tn9 integration is associated with a direct repeat of 9 base pairs (bp) of host sequence. We show that these extra 9 nucleotide pairs are generated upon insertion and not brought in with the element.
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“In some eubacteria and archaea, such as Acinetobacter baumannii, Escherichia coli, Enterococcus faecium, Lactococcus lactis, and Mycobacterium smegmatis, transposon-mediated LGT usually generates a pair of DRs varying from several to a dozen base pairs flanking the foreign gene after its integration into the host genome.33–36”
From Whole genomic DNA sequencing and comparative genomic analysis of Arthrospira platensis: high genome plasticity and genetic diversity (2016) · cites this paper as [33]
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Cites
Experiments in molecular genetics
Miller, J. H. (1972) · Medical Entomology and Zoology · Cited by 27,157
Self-citationA new method for sequencing DNA.
Maxam, A. M., & Gilbert, W. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.2.560 · Cited by 7,929
DNA Insertion Elements, Plasmids and Episomes
Broda, P. (1978) · FEBS Letters · doi:10.1016/0014-5793(78)81148-x · Cited by 1,278
Campbell, A. (1962) · Advances in genetics · doi:10.1016/s0065-2660(08)60286-2 · Cited by 614
Genetic studies of the lac repressor
Farabaugh, P. J., Schmeissner, U., Hofer, M., & Miller, J. H. (1978) · Journal of Molecular Biology · doi:10.1016/0022-2836(78)90023-2 · Cited by 517
Self-citationGenetic studies of the lac repressor
Miller, J. H., Ganem, D., Lu, P., & Schmitz, A. (1977) · Journal of Molecular Biology · doi:10.1016/s0022-2836(77)80034-x · Cited by 495
Self-citationFarabaugh, P. J. (1978) · Nature · doi:10.1038/274765a0 · Cited by 457
Tanaka, T., & Weisblum, B. (1975) · Journal of Bacteriology · doi:10.1128/jb.121.1.354-362.1975 · Cited by 449
Roberts, R. J., & Murray, K. (1976) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409237609105456 · Cited by 411
Translocatable elements in procaryotes
Kleckner, N. E. (1977) · Cell · doi:10.1016/0092-8674(77)90313-0 · Cited by 360
Research works printed but not linked: 2
- Auerswald, 1978, Insertion of transposon Tn5 results in a nine-base-pair duplication at the insertion site, Mol. Gen. Genet.
- Johnsrud, 1978, DNA sequence of IS1, a transposable element, Mol. Gen. Genet.
Cited by
Sorted by citations
Sequences at the somatic recombination sites of immunoglobulin light-chain genes
Sakano, H., Huppi, K. E., Heinrich, G., & Tonegawa, S. (1979) · Nature · doi:10.1038/280288a0 · Cited by 857
Calos, M. P., & Miller, J. H. (1980) · Cell · doi:10.1016/0092-8674(80)90305-0 · Cited by 595
Self-citationShapiro, J. A. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.4.1933 · Cited by 511
Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9
Alton, N. K., & Vapnek, D. (1979) · Nature · doi:10.1038/282864a0 · Cited by 480
Heffron, F., McCarthy, B. J., Ohtsubo, H., & Ohtsubo, E. (1979) · Cell · doi:10.1016/0092-8674(79)90228-9 · Cited by 461
Metadata
- Authors
- Johnsrud, L., Calos, M. P., & Miller, J. H.
- Affiliations
- Harvard University (US)
- University of Geneva (CH)
- ORCID
- Miller, Jeffrey H.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1978-12
- Volume
- 15
- Issue
- 4
- Pages
- 1209-1219
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2078532746
- Cited by
- 58
- References
- 44
- Citations by year
- 2024: 1 · 2019: 1 · 2016: 1 · 2015: 1
- FWCI
- 12.22
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
- Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
- Keywords
- transposon insertion, lac operon, Escherichia coli, transposon insertion sites
- MeSH terms
- Base Sequence, Chromosome Deletion, Chromosome Mapping, DNA Restriction Enzymes, Escherichia coli, Lac Operon, Translocation, Genetic
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- CoChat. (2026). Citation check: The transposon Tn9 generates a 9 bp repeated sequence during integration. https://app.cochat.ai/papers/10.1016/0092-8674(78)90047-8
- The paper
- Johnsrud, L., Calos, M. P., & Miller, J. H. (1978). The transposon Tn9 generates a 9 bp repeated sequence during integration. Cell. https://doi.org/10.1016/0092-8674(78)90047-8
