In vitro splicing of the ribosomal RNA precursor in nuclei of tetrahymena

Zaug, Arthur J.; Cech, Thomas R.

doi:10.1016/0092-8674(80)90507-3

Cited by 116RNA Research and SplicingNuclear Structure and FunctionRNA and protein synthesis mechanisms

In vitro splicing of the ribosomal RNA precursor in nuclei of tetrahymena

Zaug, Arthur J., Cech, Thomas R.·1980·Cell

DOI 10.1016/0092-8674(80)90507-3

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Abstract

The macronuclear rRNA genes of Tetrahymena thermophila contain a 0.4 kb intervening sequence. In this paper we present evidence that the excision of the intervening sequence from pre-rRNA occurs in vitro in isolated T. thermophila nuclei. The transcription-processing system includes alpha-amanitin to inhibit non-rRNA synthesis and aurintricarboxylic acid to inhibit endogenous nucleases. A discrete 0.4 kb RNA comprises up to 6% of the RNA synthesized in this system. Southern hybridization with restriction fragments of the rDNA (rRNA genes) shows that the 0.4 kb RNA contains the intervening sequence. The size of the 0.4 kb RNA, 410-425 nucleotides, is the size predicted if the entire intervening sequence were excised as a single linear molecule. The 0.4 kb RNA accumulates post-transcriptionally and appears to be stable in vitro. T. pigmentosa strain 6UM, whose rDNA has an intervening sequence of the same size and location as that of T. thermophila, also produces a 0.4 kb RNA in vitro; T. pigmentosa strain 8ALP, with not rDNA intervening sequence, produces no such RNA. The measurement of the accumulation of the excised intervening sequence is a convenient assay for the pre-rRNA splicing activity and a means for characterizing some of the details of the reaction. The resistance of the splicing acticity to concentrations of aurintricarboxylic acid that inhibit other endogenous nucleases should be useful in assaying the splicing enzyme during purification.

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Southern, E. M. (1975) · Journal of Molecular Biology · doi:10.1016/s0022-2836(75)80083-0 · Cited by 32,245 · see citing works
Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries.
Breathnach, R., Benoist, C. O., O'Hare, K., Gannon, F., & Chambon, P. (1978) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.75.10.4853 · Cited by 1,123 · see citing works
Sequence of a mouse germ-line gene for a variable region of an immunoglobulin light chain.
Tonegawa, S., Maxam, A. M., Tizard, R., Bernard, O., & Gilbert, W. (1978) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.75.3.1485 · Cited by 414 · see citing works
RNA Processing and the Intervening Sequence Problem
Abelson, J. N. (1979) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.48.070179.005131 · Cited by 354 · see citing works
The structural organization of ribosomal DNA in drosophila melanogaster
Wellauer, P. K., & Dawid, I. B. (1977) · Cell · doi:10.1016/0092-8674(77)90214-8 · Cited by 338 · see citing works
R loop mapping of the 18S and 28S sequences in the long and short repeating units of drosophila melanogaster rDNA
White, R. L., & Hogness, D. S. (1977) · Cell · doi:10.1016/0092-8674(77)90213-6 · Cited by 305 · see citing works
Messenger RNA in heat-shocked Drosophila cells
Spradling, A. C., Pardue, M. L., & Penman, S. (1977) · Journal of Molecular Biology · doi:10.1016/s0022-2836(77)80091-0 · Cited by 286 · see citing works
Use of aurintricarboxylic acid as an inhibitor of nucleases during nucleic acid isolation
Hallick, R. B., Chelm, B. K., Gray, P. W., & Orozco, E. M. (1977) · Nucleic Acids Research · doi:10.1093/nar/4.9.3055 · Cited by 280 · see citing works
A novel arrangement of the 18S and 28S sequences in a repeating unit of drosophila melanogaster rDNA
Glover, D. M., & Hogness, D. S. (1977) · Cell · doi:10.1016/0092-8674(77)90212-4 · Cited by 269 · see citing works
The macronuclear ribosomal DNA of Tetrahymena pyriformis is a palindrome
Karrer, K. M., & Gall, J. G. (1976) · Journal of Molecular Biology · doi:10.1016/0022-2836(76)90280-1 · Cited by 225 · see citing works
Sequence arrangement of the rDNA of Drosophila melanogaster
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Splicing of yeast tRNA precursors: structure of the reaction intermediates
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Expression of ribosomal DNA insertions in drosophila melanogaster
Long, E. O., & Dawid, I. B. (1979) · Cell · doi:10.1016/0092-8674(79)90231-9 · Cited by 162 · see citing works
Free ribosomal DNA molecules from Tetrahymena pyriformis GL are giant palindromes
Engberg, J., Andersson, P., Leick, V., & Collins, J. T. (1976) · Journal of Molecular Biology · doi:10.1016/0022-2836(76)90281-3 · Cited by 158 · see citing works
Free Ribosomal RNA Genes in the Macronucleus of Tetrahymena
Gall, J. G. (1974) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.71.8.3078 · Cited by 156 · see citing works
An intervening sequence in the gene coding for 25S Ribosomal RNA of Tetrahymena pigmentosa
Wild, M. A., & Gall, J. G. (1979) · Cell · doi:10.1016/0092-8674(79)90030-8 · Cited by 142 · see citing works
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Reijnders, L., Sloof, P., Sival, J., & Borst, P. (1973) · Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis · doi:10.1016/0005-2787(73)90278-5 · Cited by 139 · see citing works
Secondary structure maps of ribosomal RNA
Wellauer, P. K., Dawid, I. B., Kelley, D. E., & Perry, R. P. (1974) · Journal of Molecular Biology · doi:10.1016/0022-2836(74)90527-0 · Cited by 128 · see citing works
The inhibition of nucleic acid-binding proteins by aurintricarboxylic acid
Blumenthal, T., & Landers, T. A. (1973) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(73)91198-4 · Cited by 125 · see citing works
A Small Number of Cistrons for Ribosomal RNA in the Germinal Nucleus of a Eukaryote, Tetrahymena pyriformis
Yao, M.-C., Kimmel, A. R., & Gorovsky, M. A. (1974) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.71.8.3082 · Cited by 117 · see citing works
Removal of RNase activity from DNase by affinity chromatography on agarose-coupled aminophenylphosphoryl-uridlne-2′(3′)-phosphate
Maxwell, I. H., Maxwell, F., & Hahn, W. E. (1977) · Nucleic Acids Research · doi:10.1093/nar/4.1.241 · Cited by 100 · see citing works
The organization and expression of the eukaryotic genome
Hindley, J. (1977) · Endeavour · doi:10.1016/0160-9327(77)90192-2 · Cited by 96 · see citing works
In vitro RNA-RNA splicing in adenovirus 2 mRNA formation.
Blanchard, J., Weber, J., Jelinek, W. R., & Darnell, J. E., Jr. (1978) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.75.11.5344 · Cited by 79 · see citing works
Introduction of Hidden Breaks during rRNA Maturation and Ageing in Tetrahymena pyriformis
Eckert, W. A., Kaffenberger, W., Krohne, G., & Franke, W. W. (1978) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1978.tb12413.x · Cited by 71 · see citing works
Extrachromosomal ribosomal RNA genes in Tetrahymena structure and evolution
Din, N., & Engberg, J. (1979) · Journal of Molecular Biology · doi:10.1016/0022-2836(79)90367-x · Cited by 67 · see citing works
Localization of transcribed regions on extrachromosomal ribosomal RNA genes of Tetrahymena thermophila by R-loop mapping.
Cech, T. R., & Rio, D. C. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.10.5051 · Cited by 66 · see citing works
Self-citation
The 5′→3′ polarity of the xenopus ribosomal RNA precursor molecule
Reeder, R. H., Higashinakagawa, T., & Miller, O. L. (1976) · Cell · doi:10.1016/0092-8674(76)90158-6 · Cited by 65 · see citing works
Autonomous rDNA molecules containing single copies of the ribosomal RNA genes in the macronucleus of Tetrahymena pyriformis
Engberg, J., Christiansen, G., & Leick, V. (1974) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(74)90463-x · Cited by 64 · see citing works
The intervening sequence in the 26s rrna coding region of t. thermophila is transcribed within the largest stable precursor for rRNA
Din, N., Engberg, J., Kaffenberger, W., & Eckert, W. A. (1979) · Cell · doi:10.1016/0092-8674(79)90069-2 · Cited by 60 · see citing works
A rapid and facile procedure for the preparation of RNA polymerase I from Acanthamoeba castellanii. Purification and subunit structure
Spindler, S. R., Duester, G., D'Alessio, J. M., & Paule, M. R. (1978) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)30441-6 · Cited by 52 · see citing works
Termination of transcription in nucleoli isolated from Tetrahymena.
Leer, J. C., Tiryaki, D., & Westergaard, O. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.11.5563 · Cited by 39 · see citing works
Transcriptional properties of nucleoli isolated from Tetrahymena
Gocke, E., Leer, J. C., NIELSEN, O. F., & Westergaard, O. (1978) · Nucleic Acids Research · doi:10.1093/nar/5.11.3993 · Cited by 32 · see citing works
Isolation of a high specific activity 35S ribosomal RNA precursor from Tetrahymena pyriformis and identification of its 5' terminus, pppAp
Niles, E. G. (1978) · Biochemistry · doi:10.1021/bi00615a035 · Cited by 32 · see citing works
Ribosome biosynthesis in Tetrahymena thermophila. III. Regulation of ribosomal RNA degradation in growing and growth arrested cells
Sutton, C. A., & Hallberg, R. L. (1979) · Journal of Cellular Physiology · doi:10.1002/jcp.1041010214 · Cited by 29 · see citing works
Pools and Turnover Rates of Nuclear Ribosomal RNA in Tetrahymena pyriformis
Leick, V., & Andersen, S. B. (1970) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1970.tb00311.x · Cited by 24 · see citing works
DNA-dependent RNA Polymerase from a Protozoan, Tetrahymena pyriformis
Higashinakagawa, T., Tashiro, F., & Mita, T. (1975) · The Journal of Biochemistry · doi:10.1093/oxfordjournals.jbchem.a130783 · Cited by 14 · see citing works
Ribosome biosynthesis in Tetrahymena thermophila. IV. Regulation of ribosomal RNA synthesis in growing and growth arrested cells
Sutton, C. A., Sylvan, P., & Hallberg, R. L. (1979) · Journal of Cellular Physiology · doi:10.1002/jcp.1041010316 · Cited by 12 · see citing works

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Cech, T. R., Zaug, A. J., & Grabowski, P. J. (1981) · Cell · doi:10.1016/0092-8674(81)90390-1 · Cited by 879 · see citing works
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Ruskin, B., Krainer, A. R., Maniatis, T., & Green, M. R. (1984) · Cell · doi:10.1016/0092-8674(84)90553-1 · Cited by 740 · see citing works
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Rates and patterns of chloroplast DNA evolution.
Clegg, M. T., Gaut, B. S., Learn, G. H., & Morton, B. R. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.15.6795 · Cited by 461 · see citing works
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Grabowski, P. J., Zaug, A. J., & Cech, T. R. (1981) · Cell · doi:10.1016/0092-8674(81)90142-2 · Cited by 196 · see citing works
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Lingner, J., Hendrick, L. L., & Cech, T. R. (1994) · Genes & Development · doi:10.1101/gad.8.16.1984 · Cited by 188 · see citing works
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Konarska, M. M., Filipowicz, W., Domdey, H., & Gross, H. J. (1981) · Nature · doi:10.1038/293112a0 · Cited by 152 · see citing works
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Fu, R., Ma, Z., Zhao, H., Jin, H. Y., Tang, Y. N., He, T., Ding, Y., Zhang, J., & Ye, D. (2023) · Analytical Chemistry · doi:10.1021/acs.analchem.3c01005 · Cited by 137 · see citing works
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Ion dependence of the Bacillus subtilis RNase P reaction.
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Non-coding RNAs: An Introduction
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Nomiyama, H., Sakaki, Y., & Takagi, Y. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.3.1376 · Cited by 50 · see citing works
Mitochondrial import of only one of three nuclear‐encoded glutamine tRNAs in Tetrahymena thermophila.
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Self-citation
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β-carboxyl catalytic effect of N-phosphoryl aspartic acid
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Constraints on the evolution of plastid introns: the group II intron in the gene encoding tRNA-Val(UAC).
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Self-citation
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Selbstspleißen und enzymatische Aktivität einer intervenierenden Sequenz der RNA von Tetrahymena (Nobel‐Vortrag)
Cech, T. R. (1990) · Angewandte Chemie · doi:10.1002/ange.19901020705 · Cited by 28 · see citing works
Self-citation
Phylogenetic relationships and altered genome structures amongTetrahymenamitochondrial DNAs
Morin, G. B., & Cech, T. R. (1988) · Nucleic Acids Research · doi:10.1093/nar/16.1.327 · Cited by 28 · see citing works
Self-citation
RNA as a Versatile and Powerful Platform for Engineering Genetic Regulatory Tools
Win, M. N., & Smolke, C. D. (2007) · Biotechnology and Genetic Engineering Reviews · doi:10.1080/02648725.2007.10648106 · Cited by 27 · see citing works
Isolation, characterization, and postsynthetic modifications of Tetrahymena high-mobility group proteins
Levy-Wilson, B., Denker, M. S., & Ito, E. (1983) · Biochemistry · doi:10.1021/bi00276a030 · Cited by 26 · see citing works
In vitro splicing of the ribosomal RNA precursor in isolated nucleoli from Tetrahymena
Carin, M. N., Jensen, B. F., Jentsch, K. D., Leer, J. C., NIELSEN, O. F., & Westergaard, O. (1980) · Nucleic Acids Research · doi:10.1093/nar/8.23.5551 · Cited by 25 · see citing works
Two types of telomeric chromatin in Tetrahymena thermophila 1 1Edited by T. Richmond
Cohen, P., & Blackburn, E. H. (1998) · Journal of Molecular Biology · doi:10.1006/jmbi.1998.1867 · Cited by 25 · see citing works
‘Whole Organism’, Systems Biology, and Top-Down Criteria for Evaluating Scenarios for the Origin of Life
Brunk, C. F., & Marshall, C. R. (2021) · Life · doi:10.3390/life11070690 · Cited by 23 · see citing works
Self-Splicing and Enzymatic Activity of an Intervening Sequence RNA from Tetrahymena
Cech, T. R. (2004) · Bioscience Reports · doi:10.1007/s10540-005-2738-3 · Cited by 23 · see citing works
Self-citation
Aurine tricarboxylic acid, a potent metal-chelating inhibitor of NFκB–DNA binding
Sharma, R. K., Garg, B. S., Kurosaki, H., Goto, M., Otsuka, M., Yamamoto, T. S., & Inoue, J. (2000) · Bioorganic & Medicinal Chemistry · doi:10.1016/s0968-0896(00)00109-7 · Cited by 23 · see citing works
Isolation and characterization of macronuclei of Paramecium caudatum infected with the macronucleus-specific bacterum Holospora obtusa
Freiburg, M. (1985) · Journal of Cell Science · doi:10.1242/jcs.73.1.389 · Cited by 23 · see citing works
Mimicry of Biological Macromolecules by Polyaromatic Anionic Compounds
Regan, J. R., Bruno, J. G., Chang, M., Sabatino, R. D., D’Alisa, R. M., Ben‐Sasson, S. A., & Eilat, D. (1993) · Journal of Bioactive and Compatible Polymers · doi:10.1177/088391159300800402 · Cited by 20 · see citing works
Antisense Treatment of Viral Infection
Whitton, J. L. (1994) · Advances in virus research · doi:10.1016/s0065-3527(08)60331-0 · Cited by 19 · see citing works
Transport of functional messenger RNA from liver nuclei in a reconstituted cell-free system
Palayoor, T., Schumm, D. E., & Webb, T. E. (1981) · Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis · doi:10.1016/0005-2787(81)90173-8 · Cited by 19 · see citing works
Changes in chromatin structure accompany modulation of the rate of transcription of 5S ribosomal genes inTetrahymena
Pederson, D. S., Shupe, K., & Gorovsky, M. A. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.22.8489 · Cited by 19 · see citing works
Two distinct intervening sequences in different ribosomal DNA repeat units of Sciara coprophila
Renkawitz‐Pohl, R., Matsumoto, L. H., & Gerbi, S. A. (1981) · Nucleic Acids Research · doi:10.1093/nar/9.15.3747 · Cited by 18 · see citing works
Inhibition of nuclear ribonuclease activity during transcription invitro by aurintricarboxylic acid and vanadyl ribonucleoside complexes
Schulz-Harder, B., & Tata, J. R. (1982) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(82)91334-1 · Cited by 18 · see citing works
Complex endonudeolytic cleavage pattern during early events in the processing of pre-rRNA in the lower eukaryote,Tetrahymena thermophila
Kister, K.-P., Müller, B., & Eckert, W. A. (1983) · Nucleic Acids Research · doi:10.1093/nar/11.11.3487 · Cited by 17 · see citing works
Ribonuclease P of
True, H. L., & Celander, D. W. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.28.16559 · Cited by 17 · see citing works
Chromosome Odds and Ends
Gall, J. G. (2009) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.042308.113341 · Cited by 16 · see citing works
Nucleosome Positioning Is Independent of Histone H1in Vivo
Karrer, K. M., & VanNuland, T. A. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.46.33020 · Cited by 14 · see citing works
Small subunit, big science
Williamson, J. R. (2000) · Nature · doi:10.1038/35030271 · Cited by 14 · see citing works
Nucleocytoplasmic Transport of mRNA
Webb, T. E., Schumm, D. E., & Palayoor, T. (1981) · Elsevier eBooks · doi:10.1016/b978-0-12-147609-0.50013-7 · Cited by 14 · see citing works
The discovery of catalytic RNA
Abelson, J. N. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.105 · Cited by 11 · see citing works
Inactivation of a Single Eucaryotic Gene Irradiated in Vitro in Transcriptionally Active Chromatin Form
Herskind, C., & Westergaard, O. (1986) · Radiation Research · doi:10.2307/3576741 · Cited by 11 · see citing works
Question 5: On the Chemical Reality of the RNA World
Lucrezia, D. D., Anella, F., & Chiarabelli, C. (2007) · Origins of Life and Evolution of Biospheres · doi:10.1007/s11084-007-9098-x · Cited by 11 · see citing works
Simulations of SELEX against complex receptors with a condensed statistical model
Chen, C.-K., & Kuo, T.-C. (2006) · Computers & Chemical Engineering · doi:10.1016/j.compchemeng.2006.08.015 · Cited by 11 · see citing works
Sulfhydryl Protection and the Oxygen Effect on Radiation-Induced Inactivation of r-Chromatin in Vitro: Influence of an OH Scavenger: t-Butanol
Herskind, C. (1988) · Radiation Research · doi:10.2307/3577062 · Cited by 10 · see citing works
Finding aptamers and small ribozymes in unexpected places
Matylla-Kulińska, K., Boots, J. L., Zimmermann, B., & Schroeder, R. (2011) · Wiley Interdisciplinary Reviews - RNA · doi:10.1002/wrna.105 · Cited by 7 · see citing works
The “Tetrahymena pyriformis” complex of cryptic species
Simon, E. M., Nanney, D. L., & Doerder, F. P. (2007) · Topics in biodiversity and conservation · doi:10.1007/978-90-481-2801-3_10 · Cited by 7 · see citing works
Expression of cloned genes in cell-free systems and in microinjected Xenopus oocytes
Wickens, M., & Laskey, R. A. (1981) · Elsevier eBooks · doi:10.1016/b978-0-12-270301-0.50007-5 · Cited by 7 · see citing works
Ribosomal RNA Gene Expression in Tetrahymena: Transcription and RNA Splicing
Cech, T. R. (1986) · Elsevier eBooks · doi:10.1016/b978-0-12-273975-0.50011-5 · Cited by 7 · see citing works
Self-citation
Nucleocytoplasmic Transport of Ribosomal Subparticles: Interplay with the Nuclear Envelope
Wunderlich, F. T. (1981) · Elsevier eBooks · doi:10.1016/b978-0-12-147609-0.50014-9 · Cited by 7 · see citing works
Nuclei of Adenovirus 2-Infected Cells Contain an RNA Species That Corresponds to an Intron Excised Intact from mRNA Precursors
Rosenthal, R., & Raskas, H. J. (1985) · Molecular and Cellular Biology · doi:10.1128/mcb.5.5.1084 · Cited by 6 · see citing works
Opinion: The Key Steps in the Origin of Life to the Formation of the Eukaryotic Cell
Brunk, C. F., & Marshall, C. R. (2024) · Life · doi:10.3390/life14020226 · Cited by 5 · see citing works
“In 1982, this conflict In modern biology, nucleotide triphosphates ful ﬁ ll this role, particularly adenosine triphosphate (ATP). was resolved by the discovery of ribozymes, catalytic RNA molecules [114,115].”
Background
Characterization of an authentic Intermediate in the self-splicing process of ribosomal precursor RNA in macronuclei ofTetrahymena thermophila
Kister, K.-P., & Eckert, W. A. (1987) · Nucleic Acids Research · doi:10.1093/nar/15.5.1905 · Cited by 5 · see citing works
DNA Empire
Bánfalvi, G. (2009) · Springer eBooks · doi:10.1007/978-1-4020-9561-0_1 · Cited by 3 · see citing works
Schrödinger and the Possible Existence of Different Types of Life
Hernández, G. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.902212 · Cited by 3 · see citing works
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
Dülk, S.-L., & Rouskin, S. (2022) · Journal of Visualized Experiments · doi:10.3791/64820 · Cited by 3 · see citing works
Presence of the pre-mRNA for the 72K DNA binding protein in hnRNP from early adenovirus-2 infected HeLa cells
Kücherer, C., Marty, L., & Blanchard, J. (1982) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(82)91477-2 · Cited by 3 · see citing works
Computational Modeling Methods for 3D Structure Prediction of Ribozymes
Ghosh, P., Nithin, C., Joshi, A., Stefaniak, F., Wirecki, T. K., & Bujnicki, J. M. (2021) · doi:10.1002/9783527814527.ch33 · Cited by 3 · see citing works
Biosynthesis of ribosomal components and biogenesis of ribosomal particles
BIELKA, H. (1982) · Springer eBooks · doi:10.1007/978-3-642-68272-8_7 · Cited by 2 · see citing works
Expanding the RNA- and RNP-Based Regulatory World in Mammalian Cells
Kawasaki, S., Hirosawa, M., & Saito, H. (2023) · Springer eBooks · doi:10.1007/978-981-19-9776-1_77 · Cited by 1 · see citing works
Organ and species specificity of the messenger RNA transport factor
Smart-Nixon, S., Schumm, D. E., & Webb, T. E. (1983) · Comparative Biochemistry and Physiology Part B Comparative Biochemistry · doi:10.1016/0305-0491(83)90113-x · Cited by 1 · see citing works
THE CHEMICAL PROPERTIES OF N-(O,O-DIISOPROPYL)PHOSPHORYL-ARGININE
Li, Y.-F., Wang, Q., Zhao, Y., Ji, G.-J., & Li, Y. (1995) · Phosphorus, sulfur, and silicon and the related elements · doi:10.1080/10426509508027898 · Cited by 1 · see citing works

In vitro splicing of the ribosomal RNA precursor in isolated nucleoli from Tetrahymena
Carin, M. N., Jensen, B. F., Jentsch, K. D., Leer, J. C., NIELSEN, O. F., & Westergaard, O. (1980) · Nucleic Acids Research · doi:10.1093/nar/8.23.5551 · Cited by 25 · see citing works
Self‐splicing RNA and an RNA Enzyme in Tetruhymena1
Zaug, A. J., & Cech, T. R. (1987) · The Journal of Protozoology · doi:10.1111/j.1550-7408.1987.tb03204.x · Cited by 3 · see citing works
Self-citation
In vitro splicing of the ribosomal RNA precursor of tetrahymena: Involvement of a guanosine nucleotide in the excision of the intervening sequence
Cech, T. R., Zaug, A. J., & Grabowski, P. J. (1981) · Cell · doi:10.1016/0092-8674(81)90390-1 · Cited by 879 · see citing works
Self-citation
Summary
Cech, T. R., Zaug, A. J., & Grabowski, P. J. (2013)
Self-citation
Self-splicing RNA: Autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena
Kruger, K., Grabowski, P. J., Zaug, A. J., Sands, J., Gottschling, D. E., & Cech, T. R. (1982) · Cell · doi:10.1016/0092-8674(82)90414-7 · Cited by 2,331 · see citing works
Self-citation

Authors: Zaug, A. J., & Cech, T. R.
Affiliations: University of Colorado Boulder (US)
ORCID: Zaug, Arthur J.
Cech, Thomas R.
Venue: Cell
Publisher: Elsevier BV
Published: 1980-02
Volume: 19
Issue: 2
Pages: 331-338
ISSN: 0092-8674 (ISSN-L)
DOI: 10.1016/0092-8674(80)90507-3
OpenAlex: W2046737757
Cited by: 115
References: 39
Citations by year: 2026: 1 · 2024: 2 · 2023: 4 · 2022: 6 · 2021: 2 · 2019: 1 · 2018: 3 · 2017: 3 · 2016: 2 · 2015: 2 · 2014: 1
FWCI: 5.14
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Cell (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Keywords: ribosomal precursor RNA, Tetrahymena thermophila, post-transcriptional processing, aurintricarboxylic acid, alpha-amanitin
MeSH terms: Animals, Base Sequence, Cell Nucleus, DNA, Nucleic Acid Hybridization, Nucleic Acid Precursors, RNA, Ribosomal, Tetrahymena, Transcription, Genetic

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In vitro splicing of the ribosomal RNA precursor in nuclei of tetrahymena

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