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Analysis of transferrin recycling in mitotic and interphase hela cells by quantitative fluorescence microscopy

Sager, Polly R., Brown, Paul A., Berlin, Richard D.·1984·Cell
DOI 10.1016/0092-8674(84)90005-9
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Abstract

Recent findings suggest that membrane vesicle transport during mitosis may be generally inhibited. To test this, we examined the kinetics of uptake and exocytosis of RITC-transferrin in mitotic and interphase HeLa cells. We used quantitative image-intensification fluorescence microscopy to analyze the content of ligands in single cells. This technique was validated by comparison of 3H or RITC-transferrin release from interphase cells determined by microscopy or radiometry. Both methods gave a t1/2 of release of 5-6 min. The uptake of RITC-transferrin was depressed in mitotics. More importantly, we monitored the exocytosis of label during mitosis. Labeled mitotics were obtained by the progression of interphase cells into mitosis during a 50 min incubation with RITC-transferrin. After 30 min chase with unlabeled transferrin, the intensities of interphase cells approached background, whereas those of mitotic cells remained nearly constant. Thus both exocytosis and endocytosis of transferrin were exocytosis and endocytosis of transferrin were blocked during mitosis.

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Research works printed but not linked: 22

  1. Berlin, 1980, Surface functions during mitosis. II. Quantitation of pinocytosis and kinetics characterization of the mitotic cycle with a new fluorescence technique, J. Cell Biol., 85, 660, 10.1083/jcb.85.3.660 · DOI
  2. Berlin, 1978, Surface functions during mitosis. I. Phagocytosis, pinocytosis and mobility of surface-bound Con A, Cell, 15, 327, 10.1016/0092-8674(78)90002-8 · DOI
  3. Bleil, 1982, Transferrin receptor and its recycling in HeLa cells, EMBO J., 1, 351, 10.1002/j.1460-2075.1982.tb01173.x · DOI
  4. Boyd, 1972, N-acylsuccinimides as acylating agents for proteins: selective alcylation of lysine residues, Inter. J. Peptide Protein Res., 4, 117, 10.1111/j.1399-3011.1972.tb03407.x · DOI
  5. Burke, 1982, A monoclonal antibody against a 135-K Golgi membrane protein, EMBO J., 1, 1621, 10.1002/j.1460-2075.1982.tb01364.x · DOI
  6. Caras, 1980, Interactions of supernatant protein factor with components of the microsomal squalene epoxide system. Binding of supernatant protein factor to anionic phospholipids, J. Biol. Chem., 255, 3575, 10.1016/S0021-9258(19)85740-X · DOI
  7. Ciechanover, 1983, Kinetics of internalization and recycling of transferrin and the transferrin receptor in a human hepatoma cell line, J. Biol. Chem., 258, 9681, 10.1016/S0021-9258(17)44551-0 · DOI · Paper page
  8. Gennis, 1972, Singlet energytransfer studies on associating protein systems. Distance measurements on trypsin, α-chymotrypsin, and their protein inhibitors, Biochemistry, 11, 2517, 10.1021/bi00763a021 · DOI
  9. Harding, 1983, Receptor-mediated endocytosis of transferrin and recycling of the transferrin receptor in rat reticulocytes, J. Cell. Biol., 97, 329, 10.1083/jcb.97.2.329 · DOI · Paper page
  10. Hesketh, 1984, Stimulated release of histamine by a rat mast cell line is inhibited during mitosis, J. Cell Biol., 98, 2250, 10.1083/jcb.98.6.2250 · DOI
  11. Hiller, 1982, Golgi detection in mitotic and interphase cells by antibodies to secreted galactosyl transferase, Exp. Cell Res., 142, 85, 10.1016/0014-4827(82)90412-8 · DOI
  12. Hopkins, 1983, Internalization and processing of transferrin and the transferrin receptor in human carcinoma. A431 cells, J. Cell Biol., 97, 508, 10.1083/jcb.97.2.508 · DOI
  13. Karin, 1981, Receptor-mediated endocytosis of transferrin in developmentally totipotent mouse teratocarcinoma stem cells, J. Biol. Chem., 256, 3245, 10.1016/S0021-9258(19)69597-9 · DOI
  14. Klausner, 1983, Receptor-mediated endocytosis of transferrin in K562 cells, J. Biol. Chem., 258, 4715, 10.1016/S0021-9258(18)32481-5 · DOI
  15. Koppel, 1982, Surface functions during mitosis. III. Quantitative analysis of ligand-receptor movements into the cleavage furrow: diffusion vs. flow, J. Cell Biol., 93, 950, 10.1083/jcb.93.3.950 · DOI
  16. Lamb, 1983, Internalization and subcellular localization of transferrin and transferrin receptors in HeLa cells, J. Biol. Chem., 258, 8751, 10.1016/S0021-9258(18)32120-3 · DOI
  17. Lowry, 1951, Protein measurement with the Folin phenol reagent, J. Biol. Chem., 193, 265, 10.1016/S0021-9258(19)52451-6 · DOI
  18. Oliver, 1983, Surface and cytoskeletal events regulating leukocyte membrane topology, Sem. Hematol., 20, 282
  19. Ward, 1982, Regulation of HeLa cell transferrin receptors, J. Biol. Chem., 257, 10317, 10.1016/S0021-9258(18)34022-5 · DOI
  20. Warren, 1983, Newly synthesized G protein of vesicular stomatitis virus is not transported to the cell surface during mitosis, J. Cell Biol., 97, 1623, 10.1083/jcb.97.5.1623 · DOI
  21. Yamashiro, 1984, Segregation of transferrin to a mildly acidic (pH 6.5) para-Golgi compartment in the recycling pathway, Cell, 37, 789, 10.1016/0092-8674(84)90414-8 · DOI
  22. Zieve, 1980, Production of large numbers of mitotic mammalian cells by use of the reversible microtubule inhibitor nocodazole, Exp. Cell Res., 126, 397, 10.1016/0014-4827(80)90279-7 · DOI

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Metadata

Authors
Sager, P. R., Brown, P. A., & Berlin, R. D.
Venue
Cell
Publisher
Elsevier BV
Published
1984-12
Volume
39
Issue
2
Pages
275-282
ISSN
0092-8674
DOI
10.1016/0092-8674(84)90005-9
Cited by
60
References
0
Language
en
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)

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