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Progression of the cell cycle through mitosis leads to abortion of nascent transcripts
Open accessAbstract
Nascent transcripts of the Drosophila Ubx gene were detected by in situ hybridization. Following onset of expression, the progress of RNA polymerase (1.4 kb/min) across the gene was visualized as the successive appearance of hybridization signals from different positions within the transcription unit. Nascent transcripts disappeared at mitosis. Hybridization signals reappeared in the next cell cycle first with a 5' probe, and later, following a delay consistent with the transcription rate, with a 3' probe. Nascent transcripts were observed continuously in expressing cells of a mutant embryo in which cells are blocked in interphase. We conclude that progression through mitosis causes abortion of nascent transcripts and suggest that periodic abortion of transcription contributes to regulation of expression of large genes.
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“By monitoring the appearance of RNA by in situ hybridization, nuclear run‐on, slot blot, or RT–PCR after expression activation, RNA Pol II speed was estimated between 1.1 kb/min and 4.8 kb/min (Ucker & Yamamoto, 1984; Thummel et al, 1990; Shermoen & O'Farrell, 1991; O'Brien & Lis, 1993; Tennyson et al, 1995; Femino et al, 1998; Femino et al, 2003; Hanisch et al, 2013).”
From RNA polymerase II speed: a key player in controlling and adapting transcriptome composition (2021) · cites this paper as Shermoen & O'Farrell, 1991
Citations
Cites
A gene complex controlling segmentation in Drosophila
Lewis, E. B. (1978) · Nature · doi:10.1038/276565a0 · Cited by 3,813
Tautz, D., & Pfeifle, C. (1989) · Chromosoma · doi:10.1007/bf00291041 · Cited by 2,518
The embryonic development of Drosophila melanogaster
Campos‐Ortega, J. A., & Hartenstein, V. (1938) · PROTOPLASMA · doi:10.1007/bf01613769 · Cited by 2,156
Cyclin synthesis drives the early embryonic cell cycle
Murray, A. W., & Kirschner, M. W. (1989) · Nature · doi:10.1038/339275a0 · Cited by 1,177
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity
Murray, A. W., Solomon, M. J., & Kirschner, M. W. (1989) · Nature · doi:10.1038/339280a0 · Cited by 1,094
Foe, V. E., & Alberts, B. (1983) · Journal of Cell Science · doi:10.1242/jcs.61.1.31 · Cited by 1,056
Genetic control of cell division patterns in the Drosophila embryo
Edgar, B. A., & O’Farrell, P. H. (1989) · Cell · doi:10.1016/0092-8674(89)90183-9 · Cited by 577
Self-citationMitotic domains reveal early commitment of cells in Drosophila embryos
Foe, V. E. (1989) · Trends in Genetics · doi:10.1016/0168-9525(89)90120-0 · Cited by 562
Synthesis of RNA and protein during mitosis in mammalian tissue culture cells
Prescott, D. M., & Bender, M. A. (1962) · Experimental Cell Research · doi:10.1016/0014-4827(62)90176-3 · Cited by 499
The three postblastoderm cell cycles of Drosophila embryogenesis are regulated in G2 by string
Edgar, B. A., & O’Farrell, P. H. (1990) · Cell · doi:10.1016/0092-8674(90)90012-4 · Cited by 437
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White, R. (2019) · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · doi:10.3410/f.735733236.793561922
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Goll, M. G. (2020) · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · doi:10.3410/f.735172557.793573570
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Metadata
- Authors
- Shermoen, A. W., & O'Farrell, P. H.
- Affiliations
- University of California, San Francisco (US)
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1991-10-18
- Volume
- 67
- Issue
- 2
- Pages
- 303-310
- ISSN
- 0092-8674 (ISSN-L)
- PMC
- PMC2755073
- OpenAlex
- W1984688123
- Cited by
- 360
- References
- 32
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 5 · 2023: 11 · 2022: 13 · 2021: 10 · 2020: 15 · 2019: 14 · 2018: 9 · 2017: 8 · 2016: 12 · 2015: 9 · 2014: 14 · 2013: 17 · 2012: 8
- FWCI
- 7.01
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- McKnight Foundation
- National Institutes of Health (5p01hl043821-07)
- National Heart, Lung, and Blood Institute (P0 1 HL 43821)
- National Institute of General Medical Sciences (R01 GM37193, R01GM037193)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- nascent RNA, mitosis, cell cycle, RNA polymerase, Drosophila, transcriptional regulation, gene expression regulation
- MeSH terms
- Alkaline Phosphatase, Animals, Cell Nucleus, Digoxigenin, Drosophila melanogaster, Gene Expression Regulation, Genes, Homeobox, Mitosis, Mutation, Nucleic Acid Hybridization, Transcription, Genetic, DNA Probes
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