A novel 145 kd brain cytosolic protein reconstitutes Ca2+-regulated secretion in permeable neuroendocrine cells
Abstract
The regulated secretory pathway is activated by elevated cytoplasmic Ca2+; however, the components mediating Ca2+ regulation have not been identified. In semi-intact neuroendocrine cells, Ca(2+)-activated secretion is ATP- and cytosol protein-dependent. We have identified a novel brain protein, p145, as a cytosolic factor that reconstitutes Ca(2+)-activated secretion in two neuroendocrine cell types. The protein is a dimer of 145 kd subunits, exhibits Ca(2+)-dependent interaction with a hydrophobic matrix, and binds phospholipid vesicles, suggesting a membrane-associated function. A p145-specific antibody inhibits the reconstitution of Ca(2+)-activated secretion by cytosol, indicating an essential role for p145. The restricted expression of p145 in tissues exhibiting a regulated secretory pathway suggests a key role for this protein in the transduction of Ca2+ signals into vectorial membrane fusion events.
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CAPS was initially discovered as a regulator of Ca2+-triggered large dense core vesicle fusion in PC12-cells.
“Ca2+-dependent activator protein for secretion (CAPS) was initially discovered as a regulator of Ca2+-triggered large dense core vesicle fusion in PC12-cells (716).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [716]
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- Authors
- Walent, J. H., Porter, B. W., & Martin, T. F. J.
- Affiliations
- University of Wisconsin–Madison (US)
- ORCID
- Martin, Thomas F.J.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1992-09
- Volume
- 70
- Issue
- 5
- Pages
- 765-775
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2033302376
- Cited by
- 264
- References
- 0
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 3 · 2023: 3 · 2022: 3 · 2021: 3 · 2020: 2 · 2019: 6 · 2018: 5 · 2017: 9 · 2016: 7 · 2015: 3 · 2014: 4 · 2013: 4 · 2012: 7
- FWCI
- 6.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Diabetes and Digestive and Kidney Diseases (DK 40428, R01DK040428)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
- Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
- Keywords
- regulated secretory pathway, neuroendocrine cells, calcium signaling, membrane fusion, phospholipid vesicles, protein reconstitution, cytosolic factors
- MeSH terms
- Animals, Brain Chemistry, Calcium, Calcium-Binding Proteins, Cytosol, Exocytosis, Molecular Weight, Nerve Tissue Proteins, Norepinephrine, PC12 Cells, Rats
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