The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
In vitro antiviral activities of sulfated polysaccharides from a marine microalga (Cochlodinium polykrikoides) against human immunodeficiency virus and other enveloped viruses
Abstract
A marine microalga, Cochlodinium polykrikoides, produces extracellular sulfated polysaccharides. Isolation and purification of the polysaccharides were accomplished by precipitation with ethanol and Cetavlon, followed by DEAE-cellulose column chromatography (polysaccharides A1 and A2). These polysaccharides, which were homogeneous when analysed by both ultracentrifugal and electrophoretic methods, were composed of mannose, galactose, glucose and uronic acid, together with sulfate groups (S = 7-8% w/w). Both A1 and A2 inhibited the cytopathic effect of influenza virus types A and B in MDCK cells, that of respiratory syncytial virus types A and B in HEp-2 cells, that of human immunodeficiency virus type 1 in MT-4 cells; and, except A1 for herpes simplex virus type 1 and A2 for parainfluenza virus type 2 in HMV-2 cells, the cochlodinium polysaccharides showed no antiviral activity against parainfluenza virus types 2 and 3, measles virus, mumps virus or herpes simplex virus type 1 in HMV-2 cells. No cytotoxicity for host cells was observed with these polysaccharides at a concentration of 100 micrograms ml-1. Inhibitory effects on various viruses were achieved at concentrations that were not markedly inhibitory to the blood coagulation process.
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“For example, sulfated polysaccharides from the marine microalga Cochlodinium polykrikoides showed a significant in vitro antiviral activity against human immunodeficiency virus and absence of a cytotoxic effect directed against the host cells [117].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [117]
In the citing paper
“Some microalgal polysaccharides were found to show antiviral activities against retroviruses.”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [117]
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Overview of microalgal extracellular polymeric substances (EPS) and their applications
Xiao, R., & Zheng, Y. (2016) · Biotechnology Advances · doi:10.1016/j.biotechadv.2016.08.004 · Cited by 938
Marine Polysaccharides from Algae with Potential Biomedical Applications
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Metadata
- Authors
- Hasui, M., Matsuda, M., Okutani, K., & Shigeta, S.
- Affiliations
- Kagawa University (JP)
- Venue
- International Journal of Biological Macromolecules
- Publisher
- Elsevier BV
- Published
- 1995-01
- Volume
- 17
- Issue
- 5
- Pages
- 293-297
- ISSN
- 0141-8130 (ISSN-L)
- OpenAlex
- W2047601275
- Cited by
- 172
- References
- 14
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 10 · 2023: 6 · 2022: 24 · 2021: 18 · 2020: 11 · 2019: 5 · 2018: 8 · 2017: 5 · 2016: 5 · 2015: 7 · 2014: 5 · 2013: 7 · 2012: 6
- FWCI
- 3.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Biological Macromolecules (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
- Keywords
- sulfated polysaccharides, Cochlodinium polykrikoides, marine microalgae, antiviral activity, HIV-1, enveloped viruses, influenza virus, respiratory syncytial virus, herpes simplex virus type 1, parainfluenza virus type 2, uronic acid, polysaccharide purification, cytopathic effect inhibition, marine natural products
- MeSH terms
- Animals, Antiviral Agents, Cell Division, Cell Line, Chromatography, Drug Evaluation, Preclinical, Humans, Kidney, Laryngeal Neoplasms, Influenza A virus, Polysaccharides, Respiratory Syncytial Viruses, Spectrophotometry, Infrared, Sulfates, Tumor Cells, Cultured, Virus Replication, HIV-1, Simplexvirus, Eukaryota
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