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Cited by 16Trace Elements in HealthAntioxidant Activity and Oxidative StressHeavy Metal Exposure and Toxicity

Cytoprotective effect of copper(II) complexes against ethanol-induced damage to rat gastric mucosa

Alberghina, M., Lupo, G., La Spina, G., Rizzarelli, E., Mangiameli, A., Sciotto, D., Rizzarelli, E., Gulisano, M.·1992·Journal of Inorganic Biochemistry
DOI 10.1016/0162-0134(92)84013-d
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Abstract

The cytoprotective effect of various copper(II) complexes on the gastric mucosa damage induced by acute intragastric administration of ethanol was investigated. For in vitro experiments, the following copper(II) complexes were tested: Cu(II)(L-Trp)(L-Phe), Cu(II)(L-Leu)Cu(II)(L-Leu-Leu)(L-Leu), Cu(II)(L-Phe-L-Leu), Cu(II)(Gly-His-Lys), and Cu(II)(cyHis)2(ClO4)2. Inorganic copper such as CuSO4 was also tested. The free radical generating system, acting for 2 hr on cardial and fundic mucosa scrapings or mucosal microsomes, was Fe++ (20 microM)/ascorbate (0.25 mM). We found a marked inhibition to 75% of lipid peroxidation in the range 10-100 mM, regardless of whether copper was given in complexed or inorganic form. The results suggest that nontoxic copper(II)-amino acid complexes are able to neutralize oxygen-derived free radicals. In addition, copper(II) complexes suppressed membrane lipid peroxidation when mucosa homogenates were exposed to t-butyl hydroperoxide (1-20 microM) plus Fe++ (50 microM). In vivo experiments on rat stomachs, pretreated p.o. by gavage either with Cu(II)(L-Trp)(L-Phe) as paradigmatic agent or with copper sulphate at equivalent doses in the range 3-30 mg/kg body weight showed a significant decrease (30 min after 95% ethanol administration) in the number and severity of mucosal hemorrhagic lesions. In the gastric mucosa scrapings of copper-treated rats after ethanol exposure, we found that malondialdehyde and conjugated diene levels were unchanged compared to those of untreated controls; five enzyme activities released from lysosomes were near control values. In isolated mucosal cells, whether or not pretreated with 200 microM solution of either Cu(II)(L-Trp)(L-Phe) or CuSO4, the release of cathepsin D activity was also unmodified. The results suggest that the cytoprotective effect of Cu(II) complexes against ethanol-induced mucosal lesions was not associated in vivo to lipid peroxidation.

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Authors
Alberghina, M., Lupo, G., La Spina, G., Rizzarelli, E., Mangiameli, A., Sciotto, D., Rizzarelli, E., & Gulisano, M.
Affiliations
  • University of Catania (IT)
  • Institute of Genetics and Biophysics (IT)
Corresponding author
Alberghina, M.
ORCID
Venue
Journal of Inorganic Biochemistry
Publisher
Elsevier BV
Published
1992-03
Volume
45
Issue
4
Pages
245-259
ISSN
0162-0134 (ISSN-L)
DOI
10.1016/0162-0134(92)84013-d
OpenAlex
W2038847395
Cited by
16
References
30
Citations by year
2026: 1 · 2025: 1 · 2023: 1 · 2022: 2 · 2021: 1 · 2014: 2 · 2012: 2
FWCI
1.37
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Journal of Inorganic Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
Keywords
copper(II) complexes, cytoprotection, gastric mucosa, lipid peroxidation, oxygen free radicals, copper-amino acid complexes, copper sulfate, lysosomal enzymes, cathepsin D, malondialdehyde, free radical scavenging
MeSH terms
Ethanol, Amino Acid Sequence, Amino Acids, Animals, Copper, Dipeptides, Gastric Mucosa, Male, Microscopy, Electron, Scanning, Microsomes, Molecular Sequence Data, Oligopeptides, Organometallic Compounds, Rats, Inbred Strains, Lipid Peroxidation, Rats

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