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Cited by 268

The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.

Differentiation of filoviruses by electron microscopy

Geisbert, T.W., Jahrling, P.B.·1995·Virus Research
DOI 10.1016/0168-1702(95)00080-1
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Abstract

Cultured monolayers of MA-104, Vero 76, SW-13, and DBS-FRhL-2 cells were infected with Marburg (MBG), Ebola-Sudan (EBO-S), Ebola-Zaire (EBO-Z), and Ebola-Reston (EBO-R) viruses (Filoviridae, Filovirus) and examined by electron microscopy to provide ultrastructural details of morphology and morphogenesis of these potential human pathogens. Replication of each filovirus was seen in all cell systems employed. Filoviral particles appeared to enter host cells by endocytosis. Filoviruses showed a similar progression of morphogenic events, from the appearance of nascent intracytoplasmic viral inclusions to formation of mature virions budded through plasma membranes, regardless of serotype or host cell. However, ultrastructural differences were demonstrated between MBG and other filoviruses. MBG virions recovered from culture fluids were uniformly shorter in mean unit length than EBO-S, EBO-Z, or EBO-R particles. Examination of filovirus-infected cells revealed that intermediate MBG inclusions were morphologically distinct from EBO-S, EBO-Z, and EBO-R inclusions. No structural difference of viral inclusion material was observed among EBO-S, EBO-Z, and EBO-R. Immunoelectron microscopy showed that the filoviral matrix protein (VP40) and nucleoprotein (NP) accumulated in EBO-Z inclusions, and were closely associated during viral morphogenesis. These details facilitate the efficient and definitive diagnosis of filoviral infections by electron microscopy.

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Citations

Cites

Show all 17 references

Research works printed but not linked: 20

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Metadata

Authors
Geisbert, T. W., & Jahrling, P. B.
Venue
Virus Research
Publisher
Elsevier BV
Published
1995-12
Volume
39
Issue
2-3
Pages
129-150
ISSN
0168-1702 (ISSN-L)
DOI
10.1016/0168-1702(95)00080-1
OpenAlex
W1979071280
Cited by
268
References
17
FWCI
3.34
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)

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CoChat. (2026). Citation check: Differentiation of filoviruses by electron microscopy. https://app.cochat.ai/papers/10.1016/0168-1702(95)00080-1
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Geisbert, T. W., & Jahrling, P. B. (1995). Differentiation of filoviruses by electron microscopy. Virus Research. https://doi.org/10.1016/0168-1702(95)00080-1

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