Cited by 100Genetic Associations and EpidemiologyForensic and Genetic ResearchRenal Transplantation Outcomes and Treatments

HLA DQA, DQB, and DRB genotyping by oligonucleotide analysis: distribution of alleles and haplotypes in British caucasoids

Doherty, Derek G., Vaughan, Robert W., Donaldson, Peter T., Mowat, Alex P.·1992·Human Immunology

Abstract

A precise method for comprehensive HLA DQA and DQB genotyping using gene amplification and hybridization with sequence-specific oligonucleotide (SSO) probes is described. Twenty-four SSO probes were used to detect all DQ allotypes defined by nucleotide sequence variation in the second exons of the DQ genes, using a standard set of conditions for all probes at each locus. Five hundred individuals were genotyped for 8 DQA1 and 16 DQB1 alleles by using this method and for 33 alleles of the DRB1, DRB3, DRB4, and DRB5 genes by using previously described SSO probes. The 4-locus DQB1-DQA1-DRB1-DRB3/4/5 haplotypes present were characterized on the basis of known linkage disequilibrium between class II alleles. Fifty-two different haplotypes that have previously been described were further characterized at the nucleotide sequence level and two novel haplotypes were identified. The distributions of these alleles and haplotypes in 177 randomly selected healthy Caucasoid controls from the United Kingdom are reported. These results identify further haplotypic diversity in the HLA class II region, even though strong linkage disequilibrium exists between the DR and DQ gene loci.

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Crossref · 10.1016/0198-8859(92)90085-2

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Authors
Doherty, D. G., Vaughan, R. W., Donaldson, P. T., & Mowat, A. P.
Affiliations
Guy's Hospital (GB)
Corresponding author
Doherty, Derek G.
Venue
Human Immunology
Publisher
Elsevier BV
Published
1992-05
Volume
34
Issue
1
Pages
53-63
ISSN
0198-8859 (ISSN-L)
OpenAlex
W1974566654
Cited by
100
References
64
Citations by year
2024: 2 · 2023: 1 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 1 · 2016: 2 · 2014: 3 · 2012: 1
FWCI
4.32
Type
journal article
Language
en
Open access
closed
Locations
  • Human Immunology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Cystic Fibrosis Trust
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Associations and Epidemiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Forensic and Genetic Research
  • Health Sciences › Medicine › Transplantation › Renal Transplantation Outcomes and Treatments
Keywords
HLA class II, HLA-DQA1, HLA-DQB1, HLA-DRB1, HLA haplotypes, sequence-specific oligonucleotide probes, allele frequency distribution, haplotype diversity, linkage disequilibrium, novel haplotypes
MeSH terms
Adult, Alleles, Histocompatibility Antigens Class II, Autoimmune Diseases, Base Sequence, Child, Gene Frequency, Genes, MHC Class II, United Kingdom, Haplotypes, HLA-D Antigens, HLA-DQ Antigens, HLA-DR Antigens, Humans, Molecular Sequence Data, Oligonucleotide Probes, Sequence Tagged Sites, White People, HLA-DRB1 Chains, HLA-DRB3 Chains, HLA-DRB4 Chains, HLA-DRB5 Chains, HLA-DQ alpha-Chains, HLA-DQ beta-Chains

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