HLA DQA, DQB, and DRB genotyping by oligonucleotide analysis: distribution of alleles and haplotypes in British caucasoids
Abstract
A precise method for comprehensive HLA DQA and DQB genotyping using gene amplification and hybridization with sequence-specific oligonucleotide (SSO) probes is described. Twenty-four SSO probes were used to detect all DQ allotypes defined by nucleotide sequence variation in the second exons of the DQ genes, using a standard set of conditions for all probes at each locus. Five hundred individuals were genotyped for 8 DQA1 and 16 DQB1 alleles by using this method and for 33 alleles of the DRB1, DRB3, DRB4, and DRB5 genes by using previously described SSO probes. The 4-locus DQB1-DQA1-DRB1-DRB3/4/5 haplotypes present were characterized on the basis of known linkage disequilibrium between class II alleles. Fifty-two different haplotypes that have previously been described were further characterized at the nucleotide sequence level and two novel haplotypes were identified. The distributions of these alleles and haplotypes in 177 randomly selected healthy Caucasoid controls from the United Kingdom are reported. These results identify further haplotypic diversity in the HLA class II region, even though strong linkage disequilibrium exists between the DR and DQ gene loci.
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HLA-DQ6 was overrepresented within the small NMOSD cohort (9 out of 22 typed samples [41%] versus 15%-20% in Europe).
“Interestingly, HLA-DQ6 was overrepresented within our small NMOSD cohort (9 out of 22 typed samples [41%] versus 15%-20% in Europe 57,58 ; Table S5).”
From Autoantigen-specific CD4+ T cells acquire an exhausted phenotype and persist in human antigen-specific autoimmune diseases (2024) · cites this paper as [57]
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SHORT REPORT HLA-class II haplotype associations with ovarian cancer
Kirsten, K., Arndt, P. F., Wardelmann, E., Krebs, D., Kuhn, W. C., van der Ven, K., & Strasse, S. F. (2006)
A comparison of Gujarati Asian and Caucasian patients with rheumatoid arthritis
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- Authors
- Doherty, D. G., Vaughan, R. W., Donaldson, P. T., & Mowat, A. P.
- Affiliations
- Guy's Hospital (GB)
- Corresponding author
- Doherty, Derek G.
- ORCID
- Doherty, Derek G.
- Venue
- Human Immunology
- Publisher
- Elsevier BV
- Published
- 1992-05
- Volume
- 34
- Issue
- 1
- Pages
- 53-63
- ISSN
- 0198-8859 (ISSN-L)
- OpenAlex
- W1974566654
- Cited by
- 100
- References
- 64
- Citations by year
- 2024: 2 · 2023: 1 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 1 · 2016: 2 · 2014: 3 · 2012: 1
- FWCI
- 4.32
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Human Immunology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Cystic Fibrosis Trust
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Associations and Epidemiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Forensic and Genetic Research
- Health Sciences › Medicine › Transplantation › Renal Transplantation Outcomes and Treatments
- Keywords
- HLA class II, HLA-DQA1, HLA-DQB1, HLA-DRB1, HLA haplotypes, sequence-specific oligonucleotide probes, allele frequency distribution, haplotype diversity, linkage disequilibrium, novel haplotypes
- MeSH terms
- Adult, Alleles, Histocompatibility Antigens Class II, Autoimmune Diseases, Base Sequence, Child, Gene Frequency, Genes, MHC Class II, United Kingdom, Haplotypes, HLA-D Antigens, HLA-DQ Antigens, HLA-DR Antigens, Humans, Molecular Sequence Data, Oligonucleotide Probes, Sequence Tagged Sites, White People, HLA-DRB1 Chains, HLA-DRB3 Chains, HLA-DRB4 Chains, HLA-DRB5 Chains, HLA-DQ alpha-Chains, HLA-DQ beta-Chains
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