Cited by 90Pesticide Exposure and ToxicityAgricultural safety and regulationsPesticide Residue Analysis and Safety

Effect of pesticide mixtures on in vitro nervous cells: Comparison with single pesticides

Marinovich, M., Ghilardi, F., Galli, C.L.·1996·Toxicology

Abstract

The toxicity of dimethoate, azinphos-methyl, diazinon, pirimiphos methyl, organophosphorus insecticides, and benomyl (a benzimidazole fungicide) singly and in mixture was studied in a human neuroblastoma cell line, SH-SY5Y. The cells were incubated for 30 min and 4 h with pesticides at concentrations ranging from 0.4 to 100 micrograms/ml, or with the same compounds mixed as follows: (a) dimethoate-diazinon-azinphos; (b) benomyl-pirimiphos; (c) all together. Pesticides in the mixtures were at the same concentration used when tested singly. Diazinon, azinphos-methyl and pirimiphos, but not dimethoate and benomyl, inhibited acetylcholine esterase (AchE) activity, whereas all the compounds inhibited protein synthesis in the following order: benomyl > azinphos > diazinon >> pirimiphos = dimethoate. The mixtures showed a toxicity on AchE activity at a maximum equal to that of the most active compound in the mixture. On the contrary, the mixture were more toxic than the single compounds on protein synthesis, and in certain cases potentiation occurred. Therefore, we can conclude that it is not feasible to predict the toxicity of pesticide mixtures on the basis of the results of the toxicity of single components.

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Crossref · 10.1016/0300-483x(96)03303-3

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  • There is still scientific controversy about the safety of some currently permitted pesticides (e.g. organophosphorus compounds) even at levels below the MRL and complex mixtures of pesticides, as additive/synergistic effects of pesticide mixtures have been documented and safety testing of pesticide mixtures is currently not required.

    “There is still scientific controversy about the safety of some currently permitted pesticides (e.g. organophosphorus compounds) even at levels below the MRL and complex mixtures of pesticides, as additive/synergistic effects of pesticide mixtures have been documented and safety testing of pesticide mixtures is currently not required as part of the regulatory pesticide approval process( 58 – 60 ).”

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Authors
Marinovich, M., Ghilardi, F., & Galli, C. L.
Affiliations
University of Milan (IT)
Corresponding author
Marinovich, M.
Venue
Toxicology
Publisher
Elsevier BV
Published
1996-04
Volume
108
Issue
3
Pages
201-206
ISSN
0300-483X (ISSN-L)
OpenAlex
W2079316501
Cited by
90
References
20
Citations by year
2025: 1 · 2022: 5 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 2 · 2016: 3 · 2015: 3 · 2014: 5 · 2013: 4 · 2012: 4
FWCI
4.03
Type
journal article
Language
en
Open access
closed
Locations
  • Toxicology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Pesticide Exposure and Toxicity
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural safety and regulations
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Pesticide Residue Analysis and Safety
Keywords
pesticide mixtures, neurotoxicity, organophosphate insecticides, benomyl, dimethoate, azinphos-methyl, diazinon, pirimiphos-methyl, acetylcholinesterase inhibition, protein synthesis inhibition, SH-SY5Y cells, potentiation of toxicity, benzimidazole fungicides
MeSH terms
Acetylcholinesterase, Benomyl, Cholinesterase Inhibitors, Drug Combinations, Drug Interactions, Drug Synergism, Food Contamination, Fungicides, Industrial, Humans, Insecticides, Neuroblastoma, Neurons, Organothiophosphorus Compounds, Protein Biosynthesis, Tumor Cells, Cultured, Water Pollutants, Toxicity Tests

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