Cited by 51Adipose Tissue and MetabolismPeroxisome Proliferator-Activated ReceptorsLipid metabolism and biosynthesis

Regulation of UCP gene expression in brown adipocytes differentiated in primary culture. Effects of a new β-adrenoceptor agonist

Champigny, O., Holloway, B.R., Ricquier, D.·1992·Molecular and Cellular Endocrinology

Abstract

Primary cultures of precursor cells from mouse and rat brown adipose tissue (BAT) were used to study the effect of a new beta-agonist (ICI D7114) on the uncoupling protein (UCP) gene expression. ICI 215001 (the active metabolite of D7114) increased the expression of UCP and its mRNA in brown adipocytes differentiating in vitro in a dose-dependent manner. This stimulating effect was not inhibited by propranolol, a non-specific beta-antagonist, but was partially reduced by bupranolol, a beta 3-antagonist. No expression of UCP mRNA was ever induced by ICI 215001 in white adipocytes differentiated in vitro. It was concluded that the drug could affect the brown adipose cells through a beta 3-pathway. It could clearly modulate the expression of UCP in brown adipocytes differentiated in vitro, but was not able by itself to turn on the gene.

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Crossref · 10.1016/0303-7207(92)90177-8

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33 of 33 references verified0 citation errors · 0 not checkable

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Authors
Champigny, O., Holloway, B. R., & Ricquier, D.
Affiliations
Centre National de la Recherche Scientifique (FR)
Corresponding author
Champigny, O.
Venue
Molecular and Cellular Endocrinology
Publisher
Elsevier BV
Published
1992-07
Volume
86
Issue
1-2
Pages
73-82
ISSN
0303-7207 (ISSN-L)
OpenAlex
W2042856235
Cited by
51
References
33
Citations by year
2021: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 1
FWCI
3.24
Type
journal article
Language
en
Open access
closed
Locations
  • Molecular and Cellular Endocrinology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Centre National de la Recherche Scientifique
Topics
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
uncoupling proteins, brown adipocytes, brown adipose tissue, beta-adrenergic agonists, beta3-adrenergic receptor, adipogenesis, propranolol, bupranolol, white adipocytes, thermogenesis
MeSH terms
Uncoupling Protein 1, Adrenergic beta-Agonists, Animals, Adipose Tissue, Brown, Bupranolol, Carrier Proteins, Cell Differentiation, Cells, Cultured, Ion Channels, Isoproterenol, Male, Membrane Proteins, Mice, Inbred BALB C, Phenoxyacetates, Propranolol, RNA, Messenger, Gene Expression, Mitochondrial Proteins, Mice, Rats

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