Regulation of UCP gene expression in brown adipocytes differentiated in primary culture. Effects of a new β-adrenoceptor agonist
Abstract
Primary cultures of precursor cells from mouse and rat brown adipose tissue (BAT) were used to study the effect of a new beta-agonist (ICI D7114) on the uncoupling protein (UCP) gene expression. ICI 215001 (the active metabolite of D7114) increased the expression of UCP and its mRNA in brown adipocytes differentiating in vitro in a dose-dependent manner. This stimulating effect was not inhibited by propranolol, a non-specific beta-antagonist, but was partially reduced by bupranolol, a beta 3-antagonist. No expression of UCP mRNA was ever induced by ICI 215001 in white adipocytes differentiated in vitro. It was concluded that the drug could affect the brown adipose cells through a beta 3-pathway. It could clearly modulate the expression of UCP in brown adipocytes differentiated in vitro, but was not able by itself to turn on the gene.
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Catecholamines such as noradrenaline activate β3 adrenergic receptors and upregulate UCP-1 expression in mouse, rat, and human brown adipose tissues.
“These molecules are also known to upregulate UCP-1 expression in many models, including mouse [40,41], rat [42,43], and human [44] brown adipose tissues.”
From Mitochondrial Uncoupling: A Key Controller of Biological Processes in Physiology and Diseases (2019) · cites this paper as [41]
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Mitochondrial Uncoupling: A Key Controller of Biological Processes in Physiology and Diseases
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“These molecules are also known to upregulate UCP-1 expression in many models, including mouse [40,41], rat [42,43], and human [44] brown adipose tissues.”
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Self-citation
- Authors
- Champigny, O., Holloway, B. R., & Ricquier, D.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Champigny, O.
- Venue
- Molecular and Cellular Endocrinology
- Publisher
- Elsevier BV
- Published
- 1992-07
- Volume
- 86
- Issue
- 1-2
- Pages
- 73-82
- ISSN
- 0303-7207 (ISSN-L)
- OpenAlex
- W2042856235
- Cited by
- 51
- References
- 33
- Citations by year
- 2021: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 1
- FWCI
- 3.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular and Cellular Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Centre National de la Recherche Scientifique
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- uncoupling proteins, brown adipocytes, brown adipose tissue, beta-adrenergic agonists, beta3-adrenergic receptor, adipogenesis, propranolol, bupranolol, white adipocytes, thermogenesis
- MeSH terms
- Uncoupling Protein 1, Adrenergic beta-Agonists, Animals, Adipose Tissue, Brown, Bupranolol, Carrier Proteins, Cell Differentiation, Cells, Cultured, Ion Channels, Isoproterenol, Male, Membrane Proteins, Mice, Inbred BALB C, Phenoxyacetates, Propranolol, RNA, Messenger, Gene Expression, Mitochondrial Proteins, Mice, Rats
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