Dimethyl sulfoxide (DMSO) blocks GABA-induced current in rat dorsal root ganglion neurons
Abstract
The effects of dimethyl sulfoxide (DMSO) on gamma-aminobutyric acid (GABA)-induced Cl- currents of rat dorsal root ganglion neurons in primary culture were studied by the whole-cell patch-clamp technique. Bath application of 5 microM GABA evoked sustained inward and outward currents at membrane potentials of -60 mV and +30 mV, respectively, when the external and internal solutions both contained 142 mM Cl-. DMSO at concentrations of 0.3-3% (v/v) caused a dose-dependent inhibition of the inward current induced by 5 microM GABA at -60 mV. DMSO at 3% inhibited the GABA-induced inward and outward currents at -60 mV and +30 mV to similar extents (54% and 53% of the control, respectively), but the time-course of inhibition of the outward current at +30 mV was much faster than that of the inward current at -60 mV. These results suggest that DMSO suppressed the currents by interacting with GABA receptor-Cl- channel complex protein rather than by affecting the lipid-bilayer of the cell membrane.
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The final DMSO concentration did not exceed 0.15% in any of the recordings.
“The final DMSO concentration did not exceed 0.15% in any of the recordings given DMSO effects at higher concentrations on neuronal viability or interference with synaptic transmission (Nakahiro et al., 1992;Tsvyetlynska et al., 2005;Zhang et al., 2017).”
From Alcohol dependence and withdrawal increase sensitivity of central amygdalar GABAergic synapses to the glucocorticoid receptor antagonist mifepristone in male rats (2022) · cites this paper as Nakahiro et al., 1992
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Tsvyetlynska, N. A., Hill, R. H., & Grillner, S. (2005) · Journal of Neurophysiology · doi:10.1152/jn.00201.2005 · Cited by 37 · see citing works
Khom, S., Rodriguez, L., Gandhi, P., Kirson, D., Bajo, M., Oleata, C. S., Vendruscolo, L. F., Mason, B. J., & Roberto, M. (2022) · Neurobiology of Disease · doi:10.1016/j.nbd.2022.105610 · Cited by 23 · see citing works
- Authors
- Nakahiro, M., Arakawa, O., Narahashi, T., Ukai, S., Kato, Y., Nishinuma, K., & Nishimura, T.
- Affiliations
- Kagoshima University (JP)
- Northwestern University (US)
- The University of Osaka (JP)
- Corresponding author
- Nakahiro, Masanobu
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 1992-04
- Volume
- 138
- Issue
- 1
- Pages
- 5-8
- ISSN
- 0304-3940 (ISSN-L)
- OpenAlex
- W1993027122
- Cited by
- 49
- References
- 12
- Citations by year
- 2025: 3 · 2024: 2 · 2023: 4 · 2022: 2 · 2021: 2 · 2020: 2 · 2019: 2 · 2017: 1 · 2016: 2 · 2015: 2 · 2014: 1 · 2012: 1
- FWCI
- 0.54
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
- Keywords
- dimethyl sulfoxide, GABA, GABA-gated chloride channels, dorsal root ganglion neurons, chloride currents, lipid bilayers
- MeSH terms
- Animals, Dimethyl Sulfoxide, Dose-Response Relationship, Drug, gamma-Aminobutyric Acid, Ganglia, Spinal, Ion Channels, Membrane Potentials, Membrane Proteins, Neurons, Rats, Wistar, Chloride Channels, GABA Antagonists, Rats, In Vitro Techniques
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