Role of AMPA receptor desensitization and the side effects of a DMSO vehicle on reticulospinal EPSPs and locomotor activity
Abstract
Activation of the vertebrate locomotor network is mediated by glutamatergic synaptic drive, normally initiated by the brain stem. Previous investigations have studied the role of glutamate receptors, especially NMDA receptors, in generating and regulating locomotor pattern generation. Few studies, however, have focused on the role of AMPA receptors in shaping network activity, especially with regard to their rapid desensitization. It is important to determine whether AMPA receptor desensitization plays a role in regulating neuronal network activity. We examined this question on both the network and synaptic levels in the lamprey ( Lampetra fluviatilis) spinal cord using a selective and potent inhibitor of AMPA receptor desensitization, cyclothiazide (CTZ). The solvent dimethyl sulfoxide (DMSO) is commonly used to dissolve this drug, as well as many others. Unexpectedly, the vehicle alone already at 0.02%, but not at 0.01%, caused significant increases in excitatory postsynaptic potential (EPSP) amplitudes and NMDA-induced locomotor frequency. The results indicate that DMSO may have a profound influence when used ≥0.02%, a concentration 10–50 times less than that most commonly used. Subsequently we applied CTZ concentrations ≤10 μM (DMSO ≤0.01%). CTZ (1.25–5 μM) caused an appreciable and significant increase in EPSPs mediated by non-NMDA receptors and in both AMPA- and NMDA-induced locomotor frequency, but no effects on EPSPs mediated by NMDA receptors. From the effects of CTZ it is apparent that AMPA receptor desensitization plays an important role in determining locomotor frequency and that this is likely a result of its limiting function on AMPA receptor–mediated EPSPs.
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The final DMSO concentration did not exceed 0.15% in any of the recordings.
“The final DMSO concentration did not exceed 0.15% in any of the recordings given DMSO effects at higher concentrations on neuronal viability or interference with synaptic transmission (Nakahiro et al., 1992;Tsvyetlynska et al., 2005;Zhang et al., 2017).”
From Alcohol dependence and withdrawal increase sensitivity of central amygdalar GABAergic synapses to the glucocorticoid receptor antagonist mifepristone in male rats (2022) · cites this paper as Tsvyetlynska et al., 2005
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- Authors
- Tsvyetlynska, N. A., Hill, R. H., & Grillner, S.
- Affiliations
- Karolinska Institutet (SE)
- ORCID
- Grillner, Sten
- Venue
- Journal of Neurophysiology
- Publisher
- American Physiological Society
- Published
- 2005-12
- Volume
- 94
- Issue
- 6
- Pages
- 3951-3960
- ISSN
- 0022-3077 (ISSN-L)
- 1522-1598
- OpenAlex
- W2120112992
- Cited by
- 37
- References
- 50
- Citations by year
- 2024: 2 · 2023: 1 · 2022: 2 · 2020: 1 · 2019: 2 · 2018: 1 · 2016: 4 · 2015: 2 · 2013: 3 · 2012: 2
- FWCI
- 0.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Neurophysiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- AMPA receptor desensitization, cyclothiazide, reticulospinal neurons, excitatory postsynaptic potentials, lamprey spinal cord, NMDA receptor, glutamatergic neurotransmission, locomotor network
- MeSH terms
- Animals, Behavior, Animal, Benzothiadiazines, Dimethyl Sulfoxide, Dose-Response Relationship, Drug, Drug Interactions, Lampreys, Motor Activity, Nerve Net, Neurons, Spinal Cord, Synapses, Valine, N-Methylaspartate, Neural Networks, Computer, Receptors, AMPA, Excitatory Amino Acid Agonists, Excitatory Amino Acid Antagonists, Analgesics, Non-Narcotic, 6-Cyano-7-nitroquinoxaline-2,3-dione, Excitatory Postsynaptic Potentials
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