Immunocytochemical localization of estrogen receptors within neurotensin cells in the rostral preoptic area of the rat hypothalamus
Abstract
In situ hybridization procedures indicate that estrogen selectively increases neurotensin and neuromedin (NT/N) mRNA levels in the rostral preoptic area of the rat hypothalamus (RPH). Using the co-localization procedures of Axelson and Van Leeuwen, J. Neuroendocrinol., 2 (1990) 209-216, the present study examined whether NT cells in the RPH contained estrogen receptors (ER). Vibratome sections of brains from adult ovariectomized, colchicine-treated rats were first incubated with estrogen receptor antibody and stained with diaminobenzidine (DAB)-Ni+ producing a blue-black nucleus. Subsequently, NT antisera were used to provide a brown reaction product with DAB as chromogen. Approximately 25% of the NT cells in the RPH contained ER. These data support the hypothesis that NT cells in the RPH that play a role in luteinizing hormone release from the pituitary are, in part, influenced directly by estrogen feedback via nuclear ER and may act as interneurons controlling luteinizing hormone releasing hormone turnover.
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AVP-ir cells in the adult BNST and MeA express ER alpha and AR.
“In support, AVP-ir cells in the adult BNST and MeA express ER alpha and AR (Axelson, Shannon, & Van Leeuwen, 1992; Zhou, Blaustein, & De Vries, 1994).”
From Quantitative mapping reveals age and sex differences in vasopressin, but not oxytocin, immunoreactivity in the rat social behavior neural network (2017) · cites this paper as Axelson et al., 1992
Cites
Absence of oestradiol concentration in cell nuclei of LHRH-immunoreactive neurones
Shivers, B. D., Harlan, R. E., Morrell, J. I., & Pfaff, D. W. (1983) · Nature · doi:10.1038/304345a0 · Cited by 531 · see citing works
Axelson, J. F., & van Leeuwen, F. W. (1990) · Journal of Neuroendocrinology · doi:10.1111/j.1365-2826.1990.tb00852.x · Cited by 133 · see citing works
Self-citationVasopressin and oxytocin systems in the brain and upper spinal cord of <i>Macaca fascicularis</i>
Caffé, A. R., Van Ryen, P. C., Van Der Woude, T. P., & Van Leeuwen, F. W. (1989) · Journal of Comparative Neurology · doi:10.1002/cne.902870304 · Cited by 128 · see citing works
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De Vries, G. J., & Panzica, G. C. (2006) · Neuroscience · doi:10.1016/j.neuroscience.2005.07.050 · Cited by 346 · see citing works
DiBenedictis, B. T., Nussbaum, E. R., Cheung, H. K., & Veenema, A. H. (2017) · Journal of Comparative Neurology · doi:10.1002/cne.24216 · Cited by 74 · see citing works
- Authors
- Axelson, J. F., Shannon, W., & Van Leeuwen, F. W.
- Affiliations
- College of the Holy Cross (US)
- Corresponding author
- Axelson, John F.
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 1992-02
- Volume
- 136
- Issue
- 1
- Pages
- 5-9
- ISSN
- 0304-3940 (ISSN-L)
- OpenAlex
- W2050931768
- Cited by
- 41
- References
- 3
- Citations by year
- 2023: 2 · 2020: 1 · 2019: 1 · 2017: 1 · 2015: 1 · 2014: 1
- FWCI
- 3.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Keywords
- estrogen receptor, neurotensin, hypothalamus, luteinizing hormone secretion, estrogen feedback, gonadotropin-releasing hormone, ovariectomy, colchicine
- MeSH terms
- Animals, Diestrus, Estrogens, Female, Gonadotropin-Releasing Hormone, Neurotensin, Ovariectomy, Preoptic Area, Rats, Inbred Strains, Receptors, Estrogen, Receptors, Neurotransmitter, Receptors, Neurotensin, Rats
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