CoChat
Sign Up
Cited by 304Optical Imaging and Spectroscopy TechniquesNeuroscience and Neuropharmacology ResearchNeural dynamics and brain function

The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.

Imaging cell volume changes and neuronal excitation in the hippocampal slice

Andrew, R.D., Macvicar, B.A.·1994·Neuroscience
DOI 10.1016/0306-4522(94)90372-7
Open this paper in CoChat — free account

Abstract

Brain cell swelling is a consequence of seizure, ischemia or excitotoxicity. Changes in light reflectance from cortical surface are now used to monitor brain activity but these intrinsic signals are poorly understood. The objectives of this study were first, to show that changes in light transmittance were correlated with cell volume and second, to image increases in light transmittance as they related to neuronal activation. Transverse hippocampal slices from the rat were used for the study. Brief exposure (4-6 min) to hypo-osmotic artificial cerebrospinal fluid (-40 mOsm) elevated light transmittance consistently and reversibly in most regions of the slice and particularly in CA1 dendritic regions. Neither zero-Ca2+ artificial cerebrospinal fluid nor tetrodotoxin altered the transmittance increase and its subsequent reversal, suggesting that it was dependent on osmolality but independent of synaptic transmission and neuronal firing. The amplitude of the CA1 population spike evoked from Schaffer collaterals increased concomitantly with the hypo-osmotic increase in light transmittance, providing evidence that the extracellular tissue resistance increased. Hyper-osmotic artificial cerebrospinal fluid (+40 mOsm) containing impermeant mannitol consistently lowered light transmittance and the amplitude of the population spike. Glycerol (+40 mOsm), which is cell permeant, did not have an affect. Taken together these observations indicate that osmotic challenge alters light transmi…

Overview

Integrity signals

Registry

Crossref · 10.1016/0306-4522(94)90372-7

Cites

39 of 39 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Research works printed but not linked: 2

  1. Andrew, 1992, Imaging cell volume changes in rat hippocampal slices, Soc. Neurosci. Abstr., 18, 1125
  2. Henn, 1993, Optical imaging of intrinsic signals and extracellular resistance in hippocampal slices: correlative measures of cell swelling, Soc. Neurosci. Abstr., 19, 1322

Cited by

Sorted by citations

See all 304

Related

See all

Metadata

Authors
Andrew, R. D., & Macvicar, B. A.
Affiliations
  • Queen's University (CA)
  • University of Calgary (CA)
Corresponding author
Andrew, R.D.
ORCID
Venue
Neuroscience
Publisher
Elsevier BV
Published
1994-09
Volume
62
Issue
2
Pages
371-383
ISSN
0306-4522 (ISSN-L)
DOI
10.1016/0306-4522(94)90372-7
OpenAlex
W2051143596
Cited by
304
References
39
Citations by year
2026: 3 · 2025: 4 · 2024: 9 · 2023: 3 · 2022: 5 · 2021: 9 · 2020: 4 · 2019: 5 · 2018: 12 · 2017: 9 · 2016: 6 · 2015: 8 · 2014: 11 · 2013: 6 · 2012: 14
FWCI
10.34
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Optical Imaging and Spectroscopy Techniques
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
Keywords
optical transmittance, cell volume changes, neuronal excitation, hippocampal slices, osmotic challenge, cell swelling, CA1, population spike, kainic acid, action potential, excitotoxicity, glial swelling, tetrodotoxin, antidromic stimulation, glycerol, stratum radiatum, stratum oriens
MeSH terms
Animals, Axons, Brain Edema, Dendrites, Electric Stimulation, Evoked Potentials, Glycerol, Hippocampus, Hypotonic Solutions, Kainic Acid, Male, Models, Neurological, Neurons, Rats, Sprague-Dawley, Pyramidal Cells, Rats, In Vitro Techniques

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review