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Transformation of Staphylococcus epidermidis and other staphylococcal species with plasmid DNA by electroporation
Open accessAbstract
In this paper, the influence of various parameters on plasmid transformation by electroporation of Staphylococcus epidermidis Tü3298 was investigated. Cell growth conditions, various concentrations and forms of plasmid DNA, field strength, pulse duration and media for electroporation and regeneration were tested. In order to obtain optimal transformation efficiency, the cells were incubated for 30 min with DNA before pulsing. With the optimized procedure, other staphylococcal species such as S. aureus, S. staphylolyticus and S. carnosus were transformed with an efficiency up to 3 X 10(5) transformants per micrograms pC194 plasmid DNA.
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Research works printed but not linked: 18
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Cited by
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A wave of regulatory T cells into neonatal skin mediates tolerance to commensal microbes
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Rachid, S., Ohlsen, K., Witte, W., Hacker, J., & Ziebuhr, W. (2000) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.44.12.3357-3363.2000 · Cited by 373
Patti, J. M., Bremell, T., Krajewska-Pietrasik, D., Abdelnour, A., Tarkowski, A., Rydén, C., & Höök, M. (1994) · Infection and Immunity · doi:10.1128/iai.62.1.152-161.1994 · Cited by 357
Metadata
- Authors
- Augustin, J.
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 1990-01-01
- Volume
- 66
- Issue
- 1-3
- Pages
- 203-207
- ISSN
- 0378-1097 (ISSN-L)
- OpenAlex
- W1990222547
- Cited by
- 193
- References
- 0
- FWCI
- 4.99
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Transformation of Staphylococcus epidermidis and other staphylococcal species with plasmid DNA by electroporation. https://app.cochat.ai/papers/10.1016/0378-1097(90)90283-v
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- Augustin, J. (1990). Transformation of Staphylococcus epidermidis and other staphylococcal species with plasmid DNA by electroporation. FEMS Microbiology Letters. https://doi.org/10.1016/0378-1097(90)90283-v
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
