The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation
Open accessAbstract
The versatility of insertional inactivation of beta-galactosidase activity for subcloning and sequencing has been enhanced by combining a chemically synthesized oligonucleotide which specifies nine 6-bp-cutter restriction sites including BglII, XhoI, NruI, ClaI, SacI and EcoRV in various configurations with existing polylinkers to create a set of highly versatile cloning sites. These improved polylinkers have been inserted into plasmids (the pICs) for routine cloning of double-stranded DNA, and into chimeric phage/plasmids (the pICEMs) for biological production of single stranded DNA. The most versatile polylinker specifies 17 restriction sites in the beta-galactosidase alpha-complementing gene fragment. One of the new polylinkers was inserted into M13 DNA to produce a vector (M13mIC7) with nine cloning sites.
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Cites
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Cited by
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Metadata
- Authors
- Marsh, J. L., Erfle, M., & Wykes, E. J.
- Venue
- Gene
- Publisher
- Elsevier BV
- Published
- 1984-12
- Volume
- 32
- Issue
- 3
- Pages
- 481-485
- ISSN
- 0378-1119 (ISSN-L)
- OpenAlex
- W1986510350
- Cited by
- 698
- References
- 7
- FWCI
- 7.94
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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