The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Agrin released by motor neurons induces the aggregation of acetylcholine receptors at neuromuscular junctions
Abstract
To test the hypothesis that agrin mediates motor neuron-induced aggregation of acetylcholine receptors (AChRs) in skeletal muscle fibers and to determine whether the agrin active in this process is released by motor neurons, we raised polyclonal antibodies to purified ray agrin that blocked its receptor aggregating activity. When the antibodies were applied to chick motor neuron--chick myotube cocultures, they inhibited the formation of AChR aggregates at and near neuromuscular contacts, demonstrating that agrin plays a role in the induction of the aggregates. Rat motor neurons, like chick motor neurons, induce AChR aggregates on chick myotubes. This effect was not inhibited by our antibodies, indicating that, although the antibodies inhibited the activity of chick agrin, they did not have a similar effect on rat agrin. We conclude that agrin released by rat motor neurons induced the chick myotubes to aggregate AChRs.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Neuronal-specific Agrn isoforms containing the Z exons (32 and 33) are the most potent in promoting AChR aggregation74–77.”
From The neurogenetics of alternative splicing (2016) · cites this paper as [75]
Citations
Cites
Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-Sepharose
Ey, P. L., Prowse, S. J., & Jenkin, C. R. (1978) · Immunochemistry · doi:10.1016/0161-5890(78)90070-6 · Cited by 2,673
The Agrin HypothesisPreprint
McMahan, U. J. (1990) · Cold Spring Harbor Symposia on Quantitative Biology · doi:10.1101/sqb.1990.055.01.041 · Cited by 643
Self-citationIdentification of agrin, a synaptic organizing protein from Torpedo electric organ.
Nitkin, R. M., Smith, M. A., Magill, C., Fallon, J. R., Yao, Y. M., Wallace, B. G., & McMahan, U. J. (1987) · The Journal of cell biology · doi:10.1083/jcb.105.6.2471 · Cited by 469
Self-citationSynapse formation between dissociated nerve and muscle cells in low density cell cultures
Fischbach, G. D. (1972) · Developmental Biology · doi:10.1016/0012-1606(72)90023-1 · Cited by 458
Burden, S. J., Sargent, P. B., & McMahan, U. J. (1979) · The Journal of Cell Biology · doi:10.1083/jcb.82.2.412 · Cited by 355
Self-citationFrank, E., & Fischbach, G. D. (1979) · The Journal of Cell Biology · doi:10.1083/jcb.83.1.143 · Cited by 347
Structure and expression of a rat agrin
Rupp, F., Payan, D. G., Magill, C., Cowan, D. M., & Scheller, R. H. (1991) · Neuron · doi:10.1016/0896-6273(91)90177-2 · Cited by 306
The agrin gene codes for a family of basal lamina proteins that differ in function and distribution
Rüegg, M. A., Tsim, K. W. K., Horton, S. E., Kröger, S., Escher, G., Gensch, E. M., & McMahan, U. J. (1992) · Neuron · doi:10.1016/0896-6273(92)90090-z · Cited by 254
Self-citationDevelopment of Acetylcholine Receptor Clusters on Cultured Muscle Cells
Sytkowski, A. J., Vogel, Z., & Nirenberg, M. W. (1973) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.70.1.270 · Cited by 231
cDNA that encodes active agrin
Tsim, K. W. K., Rüegg, M. A., Escher, G., Kröger, S., & McMahan, U. J. (1992) · Neuron · doi:10.1016/0896-6273(92)90089-v · Cited by 200
Self-citationAgrin-like molecules at synaptic sites in normal, denervated, and damaged skeletal muscles.
Reist, N. E., Magill, C., & McMahan, U. J. (1987) · The Journal of Cell Biology · doi:10.1083/jcb.105.6.2457 · Cited by 173
Self-citationSchnaar, R. L., & Schaffner, A. (1981) · Journal of Neuroscience · doi:10.1523/jneurosci.01-02-00204.1981 · Cited by 158
Dohrmann, U., Edgar, D., Sendtner, M., & Thoenen, H. (1986) · Developmental Biology · doi:10.1016/0012-1606(86)90089-8 · Cited by 154
McMahan, U. J., & Slater, C. R. (1984) · The Journal of Cell Biology · doi:10.1083/jcb.98.4.1453 · Cited by 135
Self-citationMotor neurons contain agrin-like molecules.
Magill-Solc, C., & McMahan, U. J. (1988) · The Journal of Cell Biology · doi:10.1083/jcb.107.5.1825 · Cited by 130
Self-citationOn the mechanism of acetylcholine receptor accumulation at newly formed synapses on chick myotubes
Role, L. W., Matossian, V., O’Brien, R., & Fischbach, G. D. (1985) · Journal of Neuroscience · doi:10.1523/jneurosci.05-08-02197.1985 · Cited by 109
Fallon, J. R., & Gelfman, C. E. (1989) · The Journal of Cell Biology · doi:10.1083/jcb.108.4.1527 · Cited by 104
Miles, K., Anthony, D. T., Rubin, L. L., Greengard, P., & Huganir, R. L. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.18.6591 · Cited by 93
McMahan, U. J., & Wallace, B. G. (1989) · Developmental Neuroscience · doi:10.1159/000111903 · Cited by 83
Self-citationSynthesis and Transport of Agrin-Like Molecules in Motor Neurons
Magill-Solc, C., & McMahan, U. J. (1990) · Journal of Experimental Biology · doi:10.1242/jeb.153.1.1 · Cited by 72
Self-citationde la Porte, S., Vallette, F. M., Grassi, J. J., Vigny, M., & Koenig, J. (1986) · Developmental Biology · doi:10.1016/0012-1606(86)90044-8 · Cited by 30
Cited by
Sorted by citations
DEVELOPMENT OF THE VERTEBRATE NEUROMUSCULAR JUNCTION
Sanes, J. R., & Lichtman, J. W. (1999) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.22.1.389 · Cited by 1,532
Jones, F. S., & Jones, P. L. (2000) · Developmental Dynamics · doi:10.1002/(sici)1097-0177(200006)218:2 · Cited by 699
Defective neuromuscular synaptogenesis in agrin-deficient mutant mice.
Gautam, M., Noakes, P., Moscoso, L. M., Rupp, F., Scheller, R., Merlie, J., & Sanes, J. (1996) · Cell · doi:10.1016/s0092-8674(00)81253-2 · Cited by 941
The Receptor Tyrosine Kinase MuSK Is Required for Neuromuscular Junction Formation In Vivo
DeChiara, T. M., Bowen, D. C., Valenzuela, D. M., Simmons, M. V., Poueymirou, W. T., Thomas, S., Kinetz, E., Compton, D. L., Rojas, E., Park, J. S., et al. (1996) · Cell · doi:10.1016/s0092-8674(00)81251-9 · Cited by 920
Synaptic structure and development: The neuromuscular junction
Hall, Z. W., & Sanes, J. R. (1993) · Cell · doi:10.1016/s0092-8674(05)80031-5 · Cited by 834
Metadata
- Authors
- Reist, N. E., Werle, M. J., & McMahan, U. J.
- Affiliations
- Stanford Medicine (US)
- ORCID
- Reist, Noreen E.
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 1992-05
- Volume
- 8
- Issue
- 5
- Pages
- 865-868
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2068836997
- Cited by
- 237
- References
- 21
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 3 · 2023: 2 · 2022: 6 · 2020: 7 · 2019: 5 · 2018: 2 · 2017: 2 · 2016: 5 · 2015: 4 · 2014: 3 · 2013: 7 · 2012: 5
- FWCI
- 8.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Health Sciences › Medicine › Clinical Neurology › Myasthenia Gravis and Thymoma
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Keywords
- agrin, acetylcholine receptor aggregation, neuromuscular junction, motor neurons, polyclonal antibodies, skeletal muscle
- MeSH terms
- Animals, Antibodies, Cells, Cultured, Chickens, Motor Neurons, Nerve Tissue Proteins, Neuromuscular Junction, Receptors, Cholinergic, Agrin, Macromolecular Substances, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
