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Cited by 98Analytical chemistry methods developmentPesticide Residue Analysis and SafetyAnalytical Chemistry and Chromatography

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Application of dispersive liquid–liquid microextraction and dispersive micro-solid-phase extraction for the determination of quinolones in swine muscle by high-performance liquid chromatography with diode-array detection

Tsai, Wen-Hsien, Chuang, Hung-Yi, Chen, Ho-Hsien, Huang, Joh-Jong, Chen, Hwi-Chang, Cheng, Shou-Hsun, Huang, Tzou-Chi·2009·Analytica Chimica Acta
DOI 10.1016/j.aca.2009.10.008
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Abstract

Dispersive liquid-liquid microextraction (DLLME) and dispersive micro-solid-phase extraction (DMSPE) are two simple and low-cost sample preparation methods for liquid samples. In this work, these two methods were applied to solid tissue sample for the determination of seven quinolones by high-performance liquid chromatography with diode-array detection (HPLC-DAD). After the homogenization of the swine muscle with acetonitrile and salt-promoted partitioning, small amounts of the extract were used for the DLLME and DMSPE methods. In the DLLME approach, the target analytes in the extraction solvent were rapidly extracted into a small volume of dichloromethane for drying and the residue was reconstituted for HPLC-DAD analysis. In the DMSPE approach, the target analytes in the extraction solvent were trapped by dispersive silica-based PSA (primary and secondary amine) sorbents and desorbed into a small amount of desorption solution for HPLC-DAD analysis. Under the optimal conditions, relative recoveries were determined for swine muscle spiked 50-200 microg kg(-1) and quantification was achieved by matrix-matched calibration. The calibration curves of seven quinolones showed linearity with a correlation coefficient value above 0.998 for both approaches. Relative recoveries ranged from 93.0 to 104.7% and from 95.5 to 111.0% for DLLME and DMSPE, respectively. Limits of detection (LODs) ranged from 5.6 to 23.8 microg kg(-1) and from 7.5 to 26.3 microg kg(-1) for DLLME and DMSPE, respe…

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Metadata

Authors
Tsai, W.-H., Chuang, H.-Y., Chen, H.-H., Huang, J.-J., Chen, H.-C., Cheng, S.-H., & Huang, T.-C.
Affiliations
  • National Pingtung University of Science and Technology (TW)
  • Kaohsiung Medical University (TW)
  • Ministry of Health and Welfare (TW)
Corresponding author
Huang, Tzou-Chi
ORCID
Venue
Analytica Chimica Acta
Publisher
Elsevier BV
Published
2009-12
Volume
656
Issue
1-2
Pages
56-62
ISSN
0003-2670 (ISSN-L)
DOI
10.1016/j.aca.2009.10.008
OpenAlex
W2013652455
Cited by
98
References
2
Citations by year
2026: 3 · 2025: 2 · 2023: 2 · 2022: 3 · 2020: 5 · 2019: 7 · 2018: 6 · 2017: 8 · 2016: 8 · 2015: 9 · 2014: 11 · 2013: 5 · 2012: 14
FWCI
9.07
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Analytica Chimica Acta (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Chemistry › Analytical Chemistry › Analytical chemistry methods development
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Pesticide Residue Analysis and Safety
  • Physical Sciences › Chemistry › Analytical Chemistry › Analytical Chemistry and Chromatography
Keywords
quinolones, swine muscle, dispersive liquid-liquid microextraction, dispersive micro-solid phase extraction, veterinary drug residues, sample preparation, matrix-matched calibration, food safety
MeSH terms
Animals, Calibration, Chromatography, High Pressure Liquid, Chemical Fractionation, Muscles, Solvents, Swine, Quinolones

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CoChat. (2026). Citation check: Application of dispersive liquid–liquid microextraction and dispersive micro-solid-phase extraction for the determination of quinolones in swine muscle by high-performance liquid chromatography with diode-array detection. https://app.cochat.ai/papers/10.1016/j.aca.2009.10.008
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Tsai, W.-H., Chuang, H.-Y., Chen, H.-H., Huang, J.-J., Chen, H.-C., Cheng, S.-H., & Huang, T.-C. (2009). Application of dispersive liquid–liquid microextraction and dispersive micro-solid-phase extraction for the determination of quinolones in swine muscle by high-performance liquid chromatography with diode-array detection. Analytica Chimica Acta. https://doi.org/10.1016/j.aca.2009.10.008