Surface-enhanced Raman scattering molecular sentinel nanoprobes for viral infection diagnostics
Open accessAbstract
In this paper, we describe a surface-enhanced Raman scattering (SERS)-based detection approach, referred to as "molecular sentinel" (MS) plasmonic nanoprobes, to detect an RNA target related to viral infection. The MS method is essentially a label-free technique incorporating the SERS effect modulation scheme associated with silver nanoparticles and Raman dye-labeled DNA hairpin probes. Hybridization with target sequences opens the hairpin and spatially separates the Raman label from the silver surface thus reducing the SERS signal of the label. Herein, we have developed a MS nanoprobe to detect the human radical S-adenosyl methionine domain containing 2 (RSAD2) RNA target as a model system for method demonstration. The human RSAD2 gene has recently emerged as a novel host-response biomarker for diagnosis of respiratory infections. Our results showed that the RSAD2 MS nanoprobes exhibits high specificity and can detect as low as 1 nM target sequences. With the use of a portable Raman spectrometer and total RNA samples, we have also demonstrated for the first time the potential of the MS nanoprobe technology for detection of host-response RNA biomarkers for infectious disease diagnostics.
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- Authors
- Wang, H.-N., Fales, A. M., Zaas, A. K., Woods, C. W., Burke, T., Ginsburg, G. S., & Vo-Dinh, T.
- Affiliations
- Duke University (US)
- Venue
- Analytica Chimica Acta
- Publisher
- Elsevier BV
- Published
- 2013-07
- Volume
- 786
- Pages
- 153-158
- ISSN
- 0003-2670 (ISSN-L)
- PMC
- PMC4022285
- OpenAlex
- W1983113101
- Cited by
- 36
- References
- 38
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 3 · 2021: 2 · 2020: 2 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 3 · 2015: 7 · 2014: 5 · 2013: 2 · 2012: 1
- FWCI
- 2.01
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Analytica Chimica Acta (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of Biomedical Imaging and Bioengineering (R01EB006201)
- Topics
- Physical Sciences › Materials Science › Electronic, Optical and Magnetic Materials › Gold and Silver Nanoparticles Synthesis and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 detection and testing
- Keywords
- surface-enhanced Raman scattering, plasmonic nanoprobes, silver nanoparticles, host response biomarkers, respiratory infections, portable Raman spectrometer
- MeSH terms
- Viperin Protein, Humans, Proteins, Respiratory Tract Infections, Silver Nitrate, Spectrum Analysis, Raman, DNA Probes, Nanotechnology, Oxidoreductases Acting on CH-CH Group Donors, Metal Nanoparticles
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