The DOI matches its Crossref record; 80 of the 81 references it cites were found in a registry or an index.
Microfluidics for cell-based high throughput screening platforms—A review
Open accessAbstract
In the last decades, the basic techniques of microfluidics for the study of cells such as cell culture, cell separation, and cell lysis, have been well developed. Based on cell handling techniques, microfluidics has been widely applied in the field of PCR (Polymerase Chain Reaction), immunoassays, organ-on-chip, stem cell research, and analysis and identification of circulating tumor cells. As a major step in drug discovery, high-throughput screening allows rapid analysis of thousands of chemical, biochemical, genetic or pharmacological tests in parallel. In this review, we summarize the application of microfluidics in cell-based high throughput screening. The screening methods mentioned in this paper include approaches using the perfusion flow mode, the droplet mode, and the microarray mode. We also discuss the future development of microfluidic based high throughput screening platform for drug discovery.
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Microfluidics: Fluid physics at the nanoliter scale
Squires, T. M., & Quake, S. R. (2005) · Reviews of Modern Physics · doi:10.1103/revmodphys.77.977 · Cited by 4,507
Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography
Unger, M., Chou, H.-P., Thorsen, T., Scherer, A., & Quake, S. R. (2000) · Science · doi:10.1126/science.288.5463.113 · Cited by 4,016
Engineering Flows in Small Devices: Microfluidics Toward a Lab-on-a-Chip
Stone, H. A., Stroock, A. D., & Ajdari, A. (2003) · Annual Review of Fluid Mechanics · doi:10.1146/annurev.fluid.36.050802.122124 · Cited by 3,744
How to improve R&D productivity: the pharmaceutical industry's grand challenge
Paul, S. M., Mytelka, D. S., Dunwiddie, C. T., Persinger, C. C., Munos, B. H., Lindborg, S. R., & Schacht, A. L. (2010) · Nature Reviews Drug Discovery · doi:10.1038/nrd3078 · Cited by 3,566
Drug repositioning: identifying and developing new uses for existing drugs
Ashburn, T. T., & Thor, K. B. (2004) · Nature Reviews Drug Discovery · doi:10.1038/nrd1468 · Cited by 3,237
Microfluidic Large-Scale Integration
Thorsen, T., Maerkl, S. J., & Quake, S. R. (2002) · Science · doi:10.1126/science.1076996 · Cited by 2,265
El-Ali, J., Sorger, P. K., & Jensen, K. F. (2006) · Nature · doi:10.1038/nature05063 · Cited by 2,105
Lab-on-a-chip: microfluidics in drug discovery
Dittrich, P. S., & Manz, A. (2006) · Nature Reviews Drug Discovery · doi:10.1038/nrd1985 · Cited by 1,852
From 3D cell culture to organs-on-chips
Huh, D., Hamilton, G. A., & Ingber, D. E. (2011) · Trends in Cell Biology · doi:10.1016/j.tcb.2011.09.005 · Cited by 1,834
Physics and Applications of Microfluidics in Biology
Beebe, D. J., Mensing, G. A., & Walker, G. M. (2002) · Annual Review of Biomedical Engineering · doi:10.1146/annurev.bioeng.4.112601.125916 · Cited by 1,803
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Schneider, G. (2018) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2017.232 · Cited by 910
Screening out irrelevant cell-based models of disease
Horvath, P., Aulner, N., Bickle, M., Davies, A. M., Nery, E. D., Ebner, D., Montoya, M. C., Östling, P., Pietiäinen, V., Price, L. S., et al. (2016) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2016.175 · Cited by 525
High-Throughput Screening: today’s biochemical and cell-based approaches
Blay, V., Tolani, B., Ho, S. P., & Arkin, M. R. (2020) · Drug Discovery Today · doi:10.1016/j.drudis.2020.07.024 · Cited by 419
Droplet microfluidics: A tool for biology, chemistry and nanotechnology
Mashaghi, S., Abbaspourrad, A., Weitz, D. A., & van Oijen, A. M. (2016) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2016.05.019 · Cited by 376
Microfluidics: Innovations in Materials and Their Fabrication and Functionalization
Nielsen, J. B., Hanson, R. L., Almughamsi, H. M., Pang, C., Fish, T. R., & Woolley, A. T. (2019) · Analytical Chemistry · doi:10.1021/acs.analchem.9b04986 · Cited by 375
Metadata
- Authors
- Du, G., Fang, Q., & den Toonder, J. M. J.
- Affiliations
- Zhejiang University (CN)
- Corresponding author
- Fang, Qun
- ORCID
- Fang, Qun
- Venue
- Analytica Chimica Acta
- Publisher
- Elsevier BV
- Published
- 2016-01
- Volume
- 903
- Pages
- 36-50
- ISSN
- 0003-2670 (ISSN-L)
- OpenAlex
- W2175894202
- Semantic Scholar
- 19c0d49efc26c0f85b416a428d8d90b8d69be2e7
- Cited by
- 276
- References
- 81
- Citations by year
- 2026: 11 · 2025: 14 · 2024: 29 · 2023: 21 · 2022: 31 · 2021: 36 · 2020: 37 · 2019: 31 · 2018: 31 · 2017: 27 · 2016: 8
- FWCI
- 1.70
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Analytica Chimica Acta (publishedVersion)
- PubMed (publishedVersion)
- TU/e Research Portal (submittedVersion, other-oa)
- TU/e Research Portal (submittedVersion)
- Funders
- National Natural Science Foundation of China (20825517, 21227007, 21435004)
- Major National Science and Technology Programs (2013ZX09507005)
- Philips Research
- Topics
- Physical Sciences › Chemical Engineering › Fluid Flow and Transfer Processes › Innovative Microfluidic and Catalytic Techniques Innovation
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
- Keywords
- microfluidics, cell-based high-throughput screening, drug discovery, droplet microfluidics, microarray, organ-on-a-chip, cell isolation, circulating tumor cells, stem cell research, immunoassay, PCR
- MeSH terms
- Microfluidics, High-Throughput Screening Assays
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