The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Electrospun nanostructured scaffolds for bone tissue engineering
Abstract
The current challenge in bone tissue engineering is to fabricate a bioartificial bone graft mimicking the extracellular matrix (ECM) with effective bone mineralization, resulting in the regeneration of fractured or diseased bones. Biocomposite polymeric nanofibers containing nanohydroxyapatite (HA) fabricated by electrospinning could be promising scaffolds for bone tissue engineering. Nanofibrous scaffolds of poly-l-lactide (PLLA, 860+/-110 nm), PLLA/HA (845+/-140 nm) and PLLA/collagen/HA (310+/-125 nm) were fabricated, and the morphology, chemical and mechanical characterization of the nanofibers were evaluated using scanning electron microscopy, Fourier transform infrared spectroscopy and tensile testing, respectively. The in vitro biocompatibility of different nanofibrous scaffolds was also assessed by growing human fetal osteoblasts (hFOB), and investigating the proliferation, alkaline phosphatase activity (ALP) and mineralization of cells on different nanofibrous scaffolds. Osteoblasts were found to adhere and grow actively on PLLA/collagen/HA nanofibers with enhanced mineral deposition of 57% higher than the PLLA/HA nanofibers. The synergistic effect of the presence of an ECM protein, collagen and HA in PLLA/collagen/HA nanofibers provided cell recognition sites together with apatite for cell proliferation and osteoconduction necessary for mineralization and bone formation. The results of our study showed that the biocomposite PLLA/collagen/HA nanofibrous scaffold could be a potential substrate for the proliferation and mineralization of osteoblasts, enhancing bone regeneration.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Cell recognition sites for cell [102] | differentiation showed a synergistic | effect because of the presence of | ECM proteins, collagen, & | nano-HA.”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [102]
Citations
Cites
Electrospun nanofibrous structure: A novel scaffold for tissue engineering
Li, W., Laurencin, C. T., Caterson, E. J., Tuan, R. S., & Ko, F. (2002) · Journal of Biomedical Materials Research · doi:10.1002/jbm.10167 · Cited by 2,429
Structure and mechanical quality of the collagen–mineral nano-composite in bone
Fratzl, P., Gupta, H. S., Paschalis, E. P., & Roschger, P. (2004) · Journal of Materials Chemistry · doi:10.1039/b402005g · Cited by 1,221
Experimental investigation of the governing parameters in the electrospinning of polymer solutions
Theron, S. A., Zussman, E., & Yarin, A. L. (2004) · Polymer · doi:10.1016/j.polymer.2004.01.024 · Cited by 1,026
Osteoblast adhesion on nanophase ceramics
Webster, T. J., Siegel, R. W., & Bizios, R. (1999) · Biomaterials · doi:10.1016/s0142-9612(99)00020-4 · Cited by 927
Developing bioactive composite materials for tissue replacement
Wang, M. (2003) · Biomaterials · doi:10.1016/s0142-9612(03)00037-1 · Cited by 753
Tissue Engineering: Orthopedic Applications
Laurencin, C. T., Ambrosio, A. M. A., Borden, M. D., & Cooper, J. A. (1999) · Annual Review of Biomedical Engineering · doi:10.1146/annurev.bioeng.1.1.19 · Cited by 753
Collagen-Hydroxyapatite Composites for Hard Tissue Repair
Wahl, D. A., & Czernuszka, J. (2006) · European Cells and Materials · doi:10.22203/ecm.v011a06 · Cited by 676
Lee, C. H., Shin, H. J., Cho, I. H., Kang, Y.-M., Kim, I. A., Park, K., & Shin, J. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.04.037 · Cited by 633
Guided bone regeneration membrane made of polycaprolactone/calcium carbonate composite nano-fibers
Fujihara, K., Kotaki, M., & Ramakrishna, S. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.09.014 · Cited by 610
Self-citationHierarchically biomimetic bone scaffold materials: nano‐HA/collagen/PLA composite
Liao, S. S., Cui, F. Z., Zhang, W., & Feng, Q. L. (2004) · Journal of Biomedical Materials Research Part B: Applied Biomaterials · doi:10.1002/jbm.b.20035 · Cited by 487 · cited 1×
“Poly-L-lactide (PLLA) is a biodegradable polymer, widely utilized as a tissue engineering scaffold, in drug delivery, fixation in orthopedics and as a suitable bio-absorbable membrane [12,5].”
Flagged first, then by citations
Cited by
Sorted by citations
3D bioactive composite scaffolds for bone tissue engineering
Turnbull, G. S., Clarke, J., Picard, F., Riches, P. E., Jia, L., Han, F., Li, B., & Shu, W. (2017) · Bioactive Materials · doi:10.1016/j.bioactmat.2017.10.001 · Cited by 1,388
“niques including 3D printing [80] and electrospinning [127,195].”
BackgroundIndustrial Upscaling of Electrospinning and Applications of Polymer Nanofibers: A Review
Persano, L., Camposeo, A., Tekmen, Ç., & Pisignano, D. (2013) · Macromolecular Materials and Engineering · doi:10.1002/mame.201200290 · Cited by 973
Polymeric materials for bone and cartilage repair
Puppi, D., Chiellini, E., Piras, A. M., & Chiellini, E. (2010) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2010.01.006 · Cited by 913
Electrospinning for tissue engineering applications
Rahmati, M., Mills, D. K., Urbanska, A. M., Saeb, M. R., Venugopal, J. R., Ramakrishna, S., & Mozafari, M. (2020) · Progress in Materials Science · doi:10.1016/j.pmatsci.2020.100721 · Cited by 842
Self-citationElectrophoretic deposition of biomaterials
Boccaccini, A. R., Keim, S., Ma, R., Li, Y., & Zhitomirsky, I. (2010) · Journal of The Royal Society Interface · doi:10.1098/rsif.2010.0156.focus · Cited by 760
“In this context, EPD can be considered one of the electric field-assisted deposition methods gaining acceptability in the biomaterials field such as electrospraying (Jayasinghe et al. 2006; Ahmad et al. 2009; De Jonge et al. 2009) and electrospinning (Martins et al. 2008; Prabhakaran et al. 2009).”
Metadata
- Authors
- Prabhakaran, M. P., Venugopal, J., & Ramakrishna, S.
- Affiliations
- National University of Singapore (SG)
- Corresponding author
- Prabhakaran, Molamma P.
- Venue
- Acta Biomaterialia
- Publisher
- Elsevier BV
- Published
- 2009-10
- Volume
- 5
- Issue
- 8
- Pages
- 2884-2893
- ISSN
- 1742-7061 (ISSN-L)
- OpenAlex
- W2070366856
- Cited by
- 430
- References
- 35
- Citations by year
- 2026: 10 · 2025: 11 · 2024: 23 · 2023: 16 · 2022: 24 · 2021: 17 · 2020: 42 · 2019: 31 · 2018: 23 · 2017: 36 · 2016: 34 · 2015: 25 · 2014: 34 · 2013: 30 · 2012: 30
- FWCI
- 14.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Acta Biomaterialia (publishedVersion)
- PubMed (publishedVersion)
- National University of Singapore (submittedVersion)
- Funders
- National Medical Research Council
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
- Keywords
- electrospun nanofibers, bone tissue engineering, poly(L-lactic acid), hydroxyapatite nanoparticles, collagen, biocomposite scaffolds, osteoblast mineralization, alkaline phosphatase activity, osteoblast proliferation, bone regeneration, extracellular matrix mimicry, osteoconduction
- MeSH terms
- Absorption, Cell Line, Electrochemistry, Extracellular Matrix, Humans, Materials Testing, Osteoblasts, Osteogenesis, Particle Size, Polyesters, Polymers, Rotation, Surface Properties, Porosity, Bone Substitutes, Cell Culture Techniques, Lactic Acid, Tissue Engineering, Biomimetic Materials, Cell Enlargement, Nanostructures
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
