Cited by 430Bone Tissue Engineering MaterialsElectrospun Nanofibers in Biomedical Applicationsbiodegradable polymer synthesis and properties

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Electrospun nanostructured scaffolds for bone tissue engineering

Prabhakaran, Molamma P., Venugopal, J., Ramakrishna, S.·2009·Acta Biomaterialia

DOI 10.1016/j.actbio.2009.05.007

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Abstract

The current challenge in bone tissue engineering is to fabricate a bioartificial bone graft mimicking the extracellular matrix (ECM) with effective bone mineralization, resulting in the regeneration of fractured or diseased bones. Biocomposite polymeric nanofibers containing nanohydroxyapatite (HA) fabricated by electrospinning could be promising scaffolds for bone tissue engineering. Nanofibrous scaffolds of poly-l-lactide (PLLA, 860+/-110 nm), PLLA/HA (845+/-140 nm) and PLLA/collagen/HA (310+/-125 nm) were fabricated, and the morphology, chemical and mechanical characterization of the nanofibers were evaluated using scanning electron microscopy, Fourier transform infrared spectroscopy and tensile testing, respectively. The in vitro biocompatibility of different nanofibrous scaffolds was also assessed by growing human fetal osteoblasts (hFOB), and investigating the proliferation, alkaline phosphatase activity (ALP) and mineralization of cells on different nanofibrous scaffolds. Osteoblasts were found to adhere and grow actively on PLLA/collagen/HA nanofibers with enhanced mineral deposition of 57% higher than the PLLA/HA nanofibers. The synergistic effect of the presence of an ECM protein, collagen and HA in PLLA/collagen/HA nanofibers provided cell recognition sites together with apatite for cell proliferation and osteoconduction necessary for mineralization and bone formation. The results of our study showed that the biocomposite PLLA/collagen/HA nanofibrous scaffold could be a potential substrate for the proliferation and mineralization of osteoblasts, enhancing bone regeneration.

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“[29] have shown that with increased fiber size, the poly-L-lactic acid nanofibers have higher Young’s moduli and ultimate tensile strengths.”
Background
Electrospun polycaprolactone/hydroxyapatite/ZnO nanofibers as potential biomaterials for bone tissue regeneration
Shitole, A. A., Raut, P. W., Sharma, N., Giram, P. S., Khandwekar, A. P., & Garnaik, B. (2019) · Journal of Materials Science Materials in Medicine · doi:10.1007/s10856-019-6255-5 · Cited by 109
An assessment of biopolymer‐ and synthetic polymer‐based scaffolds for bone and vascular tissue engineering
Goonoo, N., Bhaw‐Luximon, A., Bowlin, G. L., & Jhurry, D. (2013) · Polymer International · doi:10.1002/pi.4474 · Cited by 107
Biodegradable electrospun nanofibers coated with platelet-rich plasma for cell adhesion and proliferation
Díaz-Gómez, L. A., Alvarez‐Lorenzo, C., Concheiro, Á., Silva, M., Domı́nguez, F., Sheikh, F. A., Cantu, T., Desai, R. D., Garcia, V. L., & Macossay, J. (2014) · Materials Science and Engineering C · doi:10.1016/j.msec.2014.03.065 · Cited by 103
“Moreover, the technique enables the combination of natural biomaterials, synthetic polymers and growth factors [11].”
Method
Simultaneous electrospin–electrosprayed biocomposite nanofibrous scaffolds for bone tissue regeneration
Francis, L., Venugopal, J. R., Prabhakaran, M. P., Thavasi, V., Marsano, E., & Ramakrishna, S. (2010) · Acta Biomaterialia · doi:10.1016/j.actbio.2010.05.001 · Cited by 99
Self-citation
Angiogenesis and osteogenesis enhanced by bFGFex vivogene therapy for bone tissue engineering in reconstruction of calvarial defects
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Polymer- and Hybrid-Based Biomaterials for Interstitial, Connective, Vascular, Nerve, Visceral and Musculoskeletal Tissue Engineering
Abalymov, A. A., Parakhonskiy, B. V., & Skirtach, A. G. (2020) · Polymers · doi:10.3390/polym12030620 · Cited by 100
“Also, fibro-cartilaginous region formation stimulated by calcium-based nano and microparticles of hydroxyapatite [137][68].”
Background
A bird's eye view on the use of electrospun nanofibrous scaffolds for bone tissue engineering: Current state‐of‐the‐art, emerging directions and future trends
Rezvani, Z., Venugopal, J. R., Urbanska, A. M., Mills, D. K., Ramakrishna, S., & Mozafari, M. (2016) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2016.05.014 · Cited by 97
Self-citation
Electrospun polyvinyl alcohol–collagen–hydroxyapatite nanofibers: a biomimetic extracellular matrix for osteoblastic cells
Song, W., Markel, D. C., Wang, S., Shi, T., Mao, G., & Ren, W. (2012) · Nanotechnology · doi:10.1088/0957-4484/23/11/115101 · Cited by 96
Advance of Nano-Composite Electrospun Fibers in Periodontal Regeneration
Zhuang, Y., Lin, K., & Yu, H. (2019) · Frontiers in Chemistry · doi:10.3389/fchem.2019.00495 · Cited by 95
Additively manufactured porous scaffolds by design for treatment of bone defects
Toosi, S. H., Javid‐Naderi, M. J., Tamayol, A., Ebrahimzadeh, M. A., Yaghoubian, S., & Shaegh, S. A. M. (2024) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1252636 · Cited by 94
“…al., 2016), laser sintering (Williams et al., 2005; Duan et al., 2010; Maskery et al., 2018), electrospinning (Liao et al., 2008; Jang et al., 2009; Prabhakaran et al., 2009; Di Martino et al., 2011; Yu et al., 2016; Lin et al., 2020), and recently, additive manufacturing (AM) (Valainis et al.,…”
Functionalized cell-free scaffolds for bone defect repair inspired by self-healing of bone fractures: A review and new perspectives
Li, L., Lu, H., Zhao, Y., Luo, J., Yang, L., Liu, W., & He, Q. (2019) · Materials Science and Engineering C · doi:10.1016/j.msec.2019.01.075 · Cited by 103
Mesenchymal Stem Cell Responses to Bone-Mimetic Electrospun Matrices Composed of Polycaprolactone, Collagen I and Nanoparticulate Hydroxyapatite
Phipps, M. C., Clem, W. C., Catledge, S. A., Xu, Y., Hennessy, K. M., Thomas, V., Jablonsky, M. J., Chowdhury, S., Stanishevsky, A. V., Vohra, Y. K., et al. (2011) · PLoS ONE · doi:10.1371/journal.pone.0016813 · Cited by 92
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Method
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Fabrication of core-shell structured nanofibers of poly (lactic acid) and poly (vinyl alcohol) by coaxial electrospinning for tissue engineering
Alharbi, H. F., Luqman, M., Khalil, K. A., Elnakady, Y. A., Abd-Elkader, O. H., Rady, A. M., Alharthi, N. H., & Karim, M. R. (2017) · European Polymer Journal · doi:10.1016/j.eurpolymj.2017.11.052 · Cited by 88
Calcium phosphate coated Keratin–PCL scaffolds for potential bone tissue regeneration
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Method
Electrospun chitosan/polyethylene-oxide (PEO)/halloysites (HAL) membranes for bone regeneration applications
Govindasamy, K., Dahlan, N. A., Pushpamalar, J., Goh, K. L., Chai, S., & Pasbakhsh, P. (2020) · Applied Clay Science · doi:10.1016/j.clay.2020.105601 · Cited by 85
Piezoelectric Scaffolds as Smart Materials for Bone Tissue Engineering
Zaszczyńska, A., Zabielski, K., Gradys, A., Kowalczyk, T., & Sajkiewicz, P. (2024) · Polymers · doi:10.3390/polym16192797 · Cited by 88
“[176] Bone regeneration in vivo.”
Electrospun inorganic and polymer composite nanofibers for biomedical applications
Sridhar, R., Sundarrajan, S., Venugopal, J. R., Ravichandran, R., & Ramakrishna, S. (2012) · Journal of Biomaterials Science Polymer Edition · doi:10.1080/09205063.2012.690711 · Cited by 85
Self-citation
Tuning Multi/Pluri-Potent Stem Cell Fate by Electrospun Poly( l -lactic acid)-Calcium-Deficient Hydroxyapatite Nanocomposite Mats
D’Angelo, F., Armentano, I., Cacciotti, I., Tiribuzi, R., Quattrocelli, M., Gaudio, C. D., Fortunati, E., Saino, E., Caraffa, A., Cerulli, G. G., et al. (2012) · Biomacromolecules · doi:10.1021/bm3000716 · Cited by 85
Co-electrospun gelatin-poly(l-lactic acid) scaffolds: Modulation of mechanical properties and chondrocyte response as a function of composition
Torricelli, P., Gioffrè, M., Fiorani, A., Panzavolta, S., Gualandi, C., Fini, M., Focarete, M. L., & Bigi, A. (2013) · Materials Science and Engineering C · doi:10.1016/j.msec.2013.11.050 · Cited by 84
In vitro characterization of 3D printed scaffolds aimed at bone tissue regeneration
Boga, J. C., Miguel, S. P., de Melo‐Diogo, D., Mendonça, A., Louro, R. O., & Correia, I. J. (2018) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2018.02.038 · Cited by 84
Enhanced mechanical strength and biocompatibility of electrospun polycaprolactone-gelatin scaffold with surface deposited nano-hydroxyapatite
Jaiswal, A. K., Chhabra, H., Soni, V., & Bellare, J. (2013) · Materials Science and Engineering C · doi:10.1016/j.msec.2013.02.003 · Cited by 81
Mechanics of bioinspired lamellar structured ceramic/polymer composites: Experiments and models
Askarinejad, S., & Rahbar, N. (2018) · International Journal of Plasticity · doi:10.1016/j.ijplas.2018.04.001 · Cited by 80
Improved functionalization of electrospun PLLA/gelatin scaffold by alternate soaking method for bone tissue engineering
Jaiswal, A. K., Kadam, S. S., Soni, V., & Bellare, J. R. (2013) · Applied Surface Science · doi:10.1016/j.apsusc.2012.12.152 · Cited by 79
Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis
English, A., Azeem, A., Spanoudes, K., Jones, E., Tripathi, B., Basu, N. B., McNamara, K., Tofail, S. A. M., Rooney, N., Riley, G. P., et al. (2015) · Acta Biomaterialia · doi:10.1016/j.actbio.2015.08.035 · Cited by 79
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Background
Electrospun ultrathin PBAT/nHAp fibers influenced the in vitro and in vivo osteogenesis and improved the mechanical properties of neoformed bone
Santana-Melo, G. D. F., Rodrigues, B. V. M., da Silva, E., Ricci, R., Marciano, F. R., Webster, T. J., de Vasconcellos, L. M. R., & Lobo, A. O. (2017) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2017.04.053 · Cited by 79
Preparation, characterization and biological test of 3D-scaffolds based on chitosan, fibroin and hydroxyapatite for bone tissue engineering
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Biocomposite scaffolds for bone regeneration: Role of chitosan and hydroxyapatite within poly-3-hydroxybutyrate-co-3-hydroxyvalerate on mechanical properties and in vitro evaluation
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Self-citation
Hydroxyapatite‐Incorporated Composite Gels Improve Mechanical Properties and Bioactivity of Bone Scaffolds
Suvarnapathaki, S., Wu, X., Lantigua, D., Nguyen, M. A., & Camci‐Unal, G. (2020) · Macromolecular Bioscience · doi:10.1002/mabi.202000176 · Cited by 77
Enhanced Growth and Osteogenic Differentiation of Human Osteoblast-Like Cells on Boron-Doped Nanocrystalline Diamond Thin Films
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“Nanostructured surfaces can be fabricated using various approaches, such as lithographic methods [9,10], self-assembly processes [10,11], treatment of polymers using plasma, ultraviolet light or ion irradiation [12,13], etching of polymers using acids or hydroxides [6], creation of nanofibrous membranes using an elect…”
Background

Authors: Prabhakaran, M. P., Venugopal, J., & Ramakrishna, S.
Affiliations: National University of Singapore (SG)
Corresponding author: Prabhakaran, Molamma P.
ORCID: Prabhakaran, Molamma P.
Venugopal, J.
Ramakrishna, S.
Venue: Acta Biomaterialia
Publisher: Elsevier BV
Published: 2009-10
Volume: 5
Issue: 8
Pages: 2884-2893
ISSN: 1742-7061 (ISSN-L)
DOI: 10.1016/j.actbio.2009.05.007
OpenAlex: W2070366856
Cited by: 430
References: 35
Citations by year: 2026: 10 · 2025: 11 · 2024: 23 · 2023: 16 · 2022: 24 · 2021: 17 · 2020: 42 · 2019: 31 · 2018: 23 · 2017: 36 · 2016: 34 · 2015: 25 · 2014: 34 · 2013: 30 · 2012: 30
FWCI: 14.82
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
Locations: Acta Biomaterialia (publishedVersion)
PubMed (publishedVersion)
National University of Singapore (submittedVersion)
Funders: National Medical Research Council
Topics: Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
Keywords: electrospun nanofibers, bone tissue engineering, poly(L-lactic acid), hydroxyapatite nanoparticles, collagen, biocomposite scaffolds, osteoblast mineralization, alkaline phosphatase activity, osteoblast proliferation, bone regeneration, extracellular matrix mimicry, osteoconduction
MeSH terms: Absorption, Cell Line, Electrochemistry, Extracellular Matrix, Humans, Materials Testing, Osteoblasts, Osteogenesis, Particle Size, Polyesters, Polymers, Rotation, Surface Properties, Porosity, Bone Substitutes, Cell Culture Techniques, Lactic Acid, Tissue Engineering, Biomimetic Materials, Cell Enlargement, Nanostructures

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Electrospun nanostructured scaffolds for bone tissue engineering

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