The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Covalently immobilized platelet-derived growth factor-BB promotes angiogenesis in biomimetic poly(ethylene glycol) hydrogels
Open accessAbstract
The field of tissue engineering is severely limited by a lack of microvascularization in tissue engineered constructs. Biomimetic poly(ethylene glycol) hydrogels containing covalently immobilized platelet-derived growth factor BB (PDGF-BB) were developed to promote angiogenesis. Poly(ethylene glycol) hydrogels resist protein absorption and subsequent non-specific cell adhesion, thus providing a “blank slate”, which can be modified through the incorporation of cell adhesive ligands and growth factors. PDGF-BB is a key angiogenic protein able to support neovessel stabilization by inducing functional anastomoses and recruiting pericytes. Due to the widespread effects of PDGF in the body and a half-life of only 30 min in circulating blood, immobilization of PDGF-BB may be necessary. In this work bioactive, covalently immobilized PDGF-BB was shown to induce tubulogenesis on two-dimensional modified surfaces, migration in three-dimensional (3D) degradable hydrogels and angiogenesis in a mouse cornea micro-pocket angiogenesis assay. Covalently immobilized PDGF-BB was also used in combination with covalently immobilized fibroblast growth factor-2, which led to significantly increased endothelial cell migration in 3D degradable hydrogels compared with the presentation of each factor alone. When a co-culture of endothelial cells and mouse pericyte precursor 10T1/2 cells was seeded onto modified surfaces tubule formation was independent of surface modifications with covalently immobilized growth factors. Furthermore, the combination of soluble PDGF-BB and immobilized PDGF-BB induced a more robust vascular response compared with soluble PDGF-BB alone when implanted into an in vivo mouse cornea micropocket angiogenesis assay. Based on these results, we believe bioactive hydrogels can be tailored to improve the formation of functional microvasculature for tissue engineering.
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Cao, R., Bråkenhielm, E., Pawliuk, R., Wariaro, D., Post, M. J., Wahlberg, E., Leboulch, P., & Cao, Y. (2003) · Nature Medicine · doi:10.1038/nm848 · Cited by 721
Creation of long-lasting blood vessels
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Recombinant Human Platelet-Derived Growth Factor: Biology and Clinical Applications
Hollinger, J. O., Hart, C. E., Hirsch, S. N., Lynch, S., & Friedlaender, G. E. (2008) · Journal of Bone and Joint Surgery · doi:10.2106/jbjs.g.01231 · Cited by 353
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Huang, G., Li, F., Zhao, X., Ma, Y., Li, Y., Lin, M., Jin, G., Lu, T. J., Genin, G. M., & Xu, F. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00094 · Cited by 825
Multifarious functions of PDGFs and PDGFRs in tumor growth and metastasis
Cao, Y. (2013) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2013.05.002 · Cited by 190
Azadi, S., Yazdanpanah, M. A., Afshari, A., Alahdad, N., Chegeni, S., Angaji, A., Rezayat, S. M., & Tavakol, S. (2024) · Journal of Tissue Engineering · doi:10.1177/20417314241303818 · Cited by 24
Metadata
- Authors
- Saik, J. E., Gould, D. J., Watkins, E. M., Dickinson, M. E., & West, J. L.
- Venue
- Acta Biomaterialia
- Publisher
- Elsevier BV
- Published
- 2011-01
- Volume
- 7
- Issue
- 1
- Pages
- 133-143
- ISSN
- 1742-7061 (ISSN-L)
- OpenAlex
- W1978290755
- Cited by
- 168
- References
- 3
- FWCI
- 4.96
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- Saik, J. E., Gould, D. J., Watkins, E. M., Dickinson, M. E., & West, J. L. (2011). Covalently immobilized platelet-derived growth factor-BB promotes angiogenesis in biomimetic poly(ethylene glycol) hydrogels. Acta Biomaterialia. https://doi.org/10.1016/j.actbio.2010.08.018
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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