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Cited by 52Bone Tissue Engineering MaterialsTissue Engineering and Regenerative MedicineMedicinal Plant Pharmacodynamics Research

Effect of age on biomaterial-mediated in situ bone tissue regeneration

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Liu, Mengqian, Nakasaki, Manando, Shih, Yu-Ru Vernon, Varghese, Shyni·2018·Acta Biomaterialia
DOI 10.1016/j.actbio.2018.06.035PMC PMC6286153
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Abstract

Emerging studies show the potential application of synthetic biomaterials that are intrinsically osteoconductive and osteoinductive as bone grafts to treat critical bone defects. Here, the biomaterial not only assists recruitment of endogenous cells, but also supports cellular activities relevant to bone tissue formation and function. While such biomaterial-mediated in situ tissue engineering is highly attractive, success of such an approach relies largely on the regenerative potential of the recruited cells, which is anticipated to vary with age. In this study, we investigated the effect of the age of the host on mineralized biomaterial-mediated bone tissue repair using critical-sized cranial defects as a model system. Mice of varying ages, 1-month-old (juvenile), 2-month-old (young-adult), 6-month-old (middle-aged), and 14-month-old (elderly), were used as recipients. Our results show that the bio-mineralized scaffolds support bone tissue formation by recruiting endogenous cells for all groups albeit with differences in an age-related manner. Analyses of bone tissue formation after 2 and 8 weeks post-treatment show low mineral deposition and reduced number of osteocalcin and tartrate-resistant acid phosphatase (TRAP)-expressing cells in elderly mice. Statement of significance Tissue engineering strategies that promote tissue repair through recruitment of endogenous cells will have a significant impact in regenerative medicine. Previous studies from our group have shown that biomineralized materials containing calcium phosphate minerals can contribute to neo-bone tissue through recruitment and activation of endogenous cells. In this study, we investigated the effect of age of the recipient on biomaterial-mediated bone tissue repair. Our results show that the age of the recipient mouse had a significant impact on the quality and quantity of the engineered neo-bone tissues, in which delayed/compromised bone tissue formation was observed in older mice. These findings are in agreement with the clinical observations that age of patients is a key factor in bone repair.

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  • For this reason, sex, age, and comorbidities are also taken into consideration, thus allowing personalized TE.

    “For this reason, sex, age, and comorbidities are also taken into consideration, thus allowing personalized TE [202].”

    From Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review (2023) · cites this paper as [202]

    The Introduction identifies recipient age as a host-specific factor in biomaterial-mediated bone repair, and the Conclusion reports that age significantly affected bone formation in mice. The paper does not establish that sex or comorbidities are taken into consideration, or that considering these factors enables personalized tissue engineering. (LLM, conf=0.87)

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  • The activity of the immune system, together with the body's regenerative capacity, greatly differs within individuals.

    “Additionally, immunoengineering also distinguishes that the activity of the immune system, together with the body’s regenerative capacity, greatly differs within individuals.”

    From Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review (2023) · cites this paper as [202]

    The Discussion reports that bone regeneration differed substantially between mice of different ages and suggests that age-related changes in inflammatory environments could influence bone formation. It does not establish that immune-system activity itself differs greatly among individuals; its findings concern age-related bone repair in mice. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

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Cited by

Sorted by citations

Authors
Liu, M., Nakasaki, M., Shih, Y.-R. V., & Varghese, S.
Affiliations
  • Duke University (US)
  • University of California San Diego (US)
Corresponding author
Varghese, Shyni
ORCID
Venue
Acta Biomaterialia
Publisher
Elsevier BV
Published
2018-09
Volume
78
Pages
329-340
ISSN
1742-7061 (ISSN-L)
DOI
10.1016/j.actbio.2018.06.035
PMC
PMC6286153
OpenAlex
W2810718827
Cited by
52
References
3
Citations by year
2026: 6 · 2025: 4 · 2024: 13 · 2023: 6 · 2022: 3 · 2021: 8 · 2020: 8 · 2019: 4
FWCI
1.95
Type
journal article
Language
en
Open access
Licence
Locations
  • Acta Biomaterialia (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR071552, R01AR063184)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Medicinal Plant Pharmacodynamics Research
Keywords
calcium phosphate, osteocalcin, TRAP-positive osteoclasts, bone grafting, osteoconduction, osteoinduction
MeSH terms
Aging, Animals, Biocompatible Materials, Bone and Bones, Bone Regeneration, Female, Mice, Inbred C57BL, Osteogenesis, Skull, Biomarkers, Neovascularization, Physiologic

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