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Detection of profilin in SPT extracts that are supposed to contain it

Pitsios, C., Iliopoulou, A., Kontogianni, M., Papagregoriou, G.·2019·Allergologia et Immunopathologia
DOI 10.1016/j.aller.2018.05.003
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Abstract

Introduction and objectives Profilin is a panallergen contained in pollen, plant foods and latex. Although cross-reactivity is expected while performing skin prick tests (SPT) with allergens that contain profilin, this is not always noticed. The purpose of this study was to detect if profilin is contained in the commercial SPT extracts of pollen and plant foods which, in their fresh form, contain determined epitopes of profilin. Material and methods Commercial SPT extracts of different pharmaceuticals were analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The study included purified palm date profilin, peach (whole, pulp and peel extracts), hazelnut, Olea europea, Parietaria judaica and Phleum pratense. Results Profilin was detected in all, but peach extracts; it was neither contained in the whole peach extract nor in the ones of peel or pulp. Conclusion The only accurate way to detect sensitization to profilin, while performing SPT, is the use of purified profilin extract. Even if a plant food or pollen contain an identified molecule of profilin, the relevant SPT commercial extract may not.

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Research works printed but not linked: 14

  1. Santos, 2011, Profilins: mimickers of allergy or relevant allergens?, Int Arch Allergy Immunol, 155, 191, 10.1159/000321178 · DOI
  2. Andersen, 2011, Identification of European allergy patterns to the allergen families PR-10 LTP, and profilin from rosaceae fruits, Clin Rev Allerg Immunol, 41, 4, 10.1007/s12016-009-8177-3 · DOI · Paper page
  3. González-Mancebo, 2011, Prevalence of sensitization to lipid transfer proteins and profilins in a population of 430 patients in the south of Madrid, J Investig Allergol Clin Immunol, 21, 278
  4. Scheurer, 2001, Cross-reactivity within the profilin panallergen family investigated by comparison of recombinant profilins from pear (Pyr c 4), cherry (Pru av 4) and celery (Api g 4) with birch pollen profilin Bet v 2, J Chromatogr B: Biomed Sci Appl, 756, 315, 10.1016/S0378-4347(01)00090-1 · DOI
  5. Asero, 2004, Parietaria profiling shows only limited cross-reactivity with birch and grass profilins, Int Arch Allergy Immunol, 133, 121, 10.1159/000076384 · DOI
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  7. Iliopoulou, 2018, Profilin sensitization and its clinical relevance to a population of atopic adults in Greece, Rev Fr Allergol, 58, 72, 10.1016/j.reval.2017.11.001 · DOI
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Metadata

Authors
Pitsios, C., Iliopoulou, A., Kontogianni, M., & Papagregoriou, G.
ORCID
Pitsios, C.
Venue
Allergologia et Immunopathologia
Publisher
Codon Publications
Published
2019-01
Volume
47
Issue
1
Pages
12-15
ISSN
0301-0546
DOI
10.1016/j.aller.2018.05.003
Cited by
6
References
0
Language
en
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)

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