Cited by 123Protein Hydrolysis and Bioactive PeptidesAquaculture Nutrition and GrowthFood and Agricultural Sciences

Utilization of tilapia processing waste for the production of fish protein hydrolysate

Silva, J.F.X., Ribeiro, K., Silva, J.F., Cahú, T.B., Bezerra, R.S.·2014·Animal Feed Science and Technology

Abstract

Viscera and carcasses represent 60–70% of whole fish body weight, and this processing waste is a known source of bioactive molecules, including proteases. These enzymes can be employed in various biotechnological processes, such as, for preparing fish protein hydrolysate (FPH). Therefore, the objective of this study was to evaluate the use of processing waste from Nile tilapia (Oreochromis niloticus) as a source of protein and proteases to produce FPH. Three FPH production conditions were evaluated: two conditions used autolysis with enzymes extracted from the tilapia intestine at different concentrations (FPH100, 100mg of tissue/mL and FPH600, 600mg of tissue/mL) and the third used 0.5% (v/v) Alcalase (FPHcom), a commercial protease preparation. Protein, amino acids and fatty acids content were calculated as DM basis. After a 4-h reaction, maximum hydrolysis percentages from FPHcom, FPH100, and FPH600 systems were 34.73±1.44%, 29.21±0.79%, and 41.66±1.33%, respectively. The protein content in the resulting FPS was 584.8g/kg, 492.3g/kg, and 508.2g/kg for FPHcom, FPH100, and FPH600, respectively. Methionine and lysine were found at levels of 32.0 and 77.0g/kg (FPHcom), 31.0 and 64.0g/kg (FPH100), and 33.0 and 69.0g/kg (FPH600), respectively. Polyunsaturated fatty acid contents of FPHcom, FPH100, and FPH600 were 101.0g/kg, 138.0g/kg, and 70g/kg, respectively, with a predominance of linoleic acid (C18:2n−6). Amino acid composition, lipid profile, and amino acid score suggested that all of the experimental FPHs could be employed as a protein source in diets for aquatic organisms and other farmed animals.

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Crossref · 10.1016/j.anifeedsci.2014.06.010

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Authors
Silva, J. F. X., Ribeiro, K., Silva, J. F., Cahú, T. B., & Bezerra, R. S.
Affiliations
  • Universidade Federal de Pernambuco (BR)
  • Universidade Federal do Rio Grande do Norte (BR)
Venue
Animal Feed Science and Technology
Publisher
Elsevier BV
Published
2014-10
Volume
196
Pages
96-106
ISSN
0377-8401 (ISSN-L)
OpenAlex
W1997895484
Cited by
123
References
45
Citations by year
2026: 5 · 2025: 11 · 2024: 14 · 2023: 9 · 2022: 6 · 2021: 20 · 2020: 9 · 2019: 14 · 2018: 9 · 2017: 12 · 2016: 9 · 2015: 5
FWCI
3.27
Type
journal article
Language
en
Open access
closed
Locations
Animal Feed Science and Technology (publishedVersion)
Funders
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
  • Ministério da Pesca e Aquicultura (MPA)
  • Fundação de Amparo a Ciência e Tecnologia do Estado de Pernambuco (FACEPE)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
Keywords
fish protein hydrolysate, Oreochromis niloticus, fish waste, fish viscera, fish carcasses, proteolytic enzymes, autolysis, alcalase, proteolysis, amino acid composition, fatty acid composition, polyunsaturated fatty acids, methionine, lysine, fish feed protein source

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