Spirulina sp. LEB 18 cultivation in outdoor pilot scale using aquaculture wastewater: High biomass, carotenoid, lipid and carbohydrate production
Abstract
Abstract The objective of this study was to integrate the treatment of aquaculture effluent with the biomass and macronutrients production by Spirulina sp. LEB 18. The first experiment was carried out in a photobioreactor (1 L) and the best biomass production condition was reproduced on a pilot scale in a raceway type bioreactor (5 L). The best treatment was T25 (100% of wastewater supplemented with 25% of Zarrouk nutrients). The biomass production in the raceway system (T25) was higher (3.33 g L−1) than the control (2.32 g L−1) and the carbohydrate (69.77%) and lipid (12.77%) were also higher. The content of carotenoids in T25 was significant (9.68 μg mL−1). Spirulina sp. LEB 18 removed phosphate (99.97%); COD (89.34%) and nitrate (81.10%) from aquaculture effluent. The effluent showed absence of heavy metals and concentration of Al; Fe; Ca and Mg higher than the control culture with Zarrouk nutrients. The Spirulina sp. LEB 18 can be applied as a bioremediation agent of aquaculture wastewater with high production of biomass, carbohydrate, lipid and carotenoid.
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Cardoso et al. documented pigment decreases in T25 treatment from small-scale photobioreactors (5 L) for Chlorophyll-a (12.48 μg/mL), b (1.41 μg/mL), and carotenoids (9.68 μg/mL).
“Cardoso et al. [41] also documented pigment decreases in T25 treatment from the small-scale photobioreactors (5 L) for Chlorophyll-a (12.48 µ g/mL), b (1.41 µ g/mL), and carotenoids (9.68 µ g/mL).”
From Strategy Development for Microalgae Spirulina platensis Biomass Cultivation in a Bubble Photobioreactor to Promote High Carbohydrate Content (2022) · cites this paper as [41]
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- Authors
- Cardoso, L. G., Duarte, J. H., Andrade, B. B., Lemos, P. V. F., Costa, J. A. V., Druzian, J. I., & Chinalia, F. A.
- Affiliations
- Universidade Federal da Bahia (BR)
- Universidade Federal do Rio Grande (BR)
- Corresponding author
- Cardoso, Lucas Guimarães
- ORCID
- Venue
- Aquaculture
- Publisher
- Elsevier BV
- Published
- 2020-08
- Volume
- 525
- Pages
- 735272
- ISSN
- 0044-8486 (ISSN-L)
- OpenAlex
- W3013173325
- Semantic Scholar
- 217b477f53abc5784cf288f20690a7def54d65cd
- Cited by
- 96
- References
- 47
- Citations by year
- 2026: 11 · 2025: 11 · 2024: 19 · 2023: 14 · 2022: 17 · 2021: 17 · 2020: 7
- FWCI
- 2.72
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Aquaculture (publishedVersion)
- Funders
- FAPESB - Foundation for Research Support (400710 / 2014-5)
- CNPQ
- MCTIC
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Environmental Science › Waste Management and Disposal › Wastewater Treatment and Reuse
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Keywords
- aquaculture wastewater, wastewater treatment, photobioreactors, microalgae cultivation, carotenoids, lipid production, carbohydrate production, phosphate removal, COD removal, nitrate removal, bioremediation
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