Colonization of oropharynx and lower respiratory tract in critical patients: risk of ventilator-associated pneumonia.
Open accessAbstract
Objective To investigate the microbial diversity existing in oral cavity and respiratory tract samples (from mini-bronchoalveolar lavage (BAL), endotracheal aspirate, and orotracheal tube) of patients on mechanical ventilation by using the checkerboard DNA-DNA hybridisation. Also, the study aimed to evaluate whether the microbial profile in the oral cavity is found in respiratory tract samples, at different periods of mechanical ventilation time (12h, 48h, 96h) in attempt to identification of relationship between VAP (ventilator-associated pneumonia) and bacterial species studied. The last objective was to analyses correlation between blood cultures and VAP.Design The samples were collected from ten patients in intensive care unit with medical prescription of orotracheal intubation and mechanical ventilation. Clinical data were tabulated and blood cultures were performed according medical indication. For checkerboard samples collection, chosen sites were the dorsal side of the tongue and gingival sulcus at 12h, 48h, 96h, BAL, at 12h, endotracheal aspirate at 48 and 96h, and orotracheal tube at extubation time, when feasible.Results It was possible to identify the presence of bacterial species in mouth and in the BAL/endotracheal aspirate. The data demonstrated an increase in the quantity of bacterial associated with prolonged use of mechanical ventilation (48 and 96h).Conclusions Bacterial species may migrate rapidly from mouth and upper airways during orotracheal intubation which contributes to the pathogenesis of VAP. There were associations between VAP and Enterococcus faecalis, Fusobacterium periodonticum, Gemella morbillorum, Neisseria mucosa, Propionibacterium acnes, Prevotella melaninogenica, Streptococcus oralis, Streptococcus sanguinis, Treponema denticola, Treponema socransckii, and Veillonella parvula.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Periodontal pathogens such as Treponema denticola, P. gingivalis, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans and Veillonella parvula have been detected in the lungs of ICU patients.
“Periodontal pathogens such as Treponema denticola, P. gingivalis, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans and Veillonella parvula, have been detected in the lungs of ICU patients (Kumar, 2017; de Carvalho Baptista et al., 2018).”
From Oral Microbiome and SARS-CoV-2: Beware of Lung Co-infection (2020) · cites this paper as de Carvalho Baptista et al., 2018
In the process of orotracheal intubation, bacteria can rapidly migrate from the oral cavity and upper respiratory tract into the lungs.
“In the process of orotracheal intubation, bacteria can rapidly migrate from the oral cavity and upper respiratory tract into the lungs (de Carvalho Baptista et al., 2018).”
From Oral Microbiome and SARS-CoV-2: Beware of Lung Co-infection (2020) · cites this paper as de Carvalho Baptista et al., 2018
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
Feinberg, A. P., & Vogelstein, B. (1983) · Analytical Biochemistry · doi:10.1016/0003-2697(83)90418-9 · Cited by 24,512 · see citing works
Device-Associated Nosocomial Infections in 55 Intensive Care Units of 8 Developing Countries
Rosenthal, V., Maki, D. G., Salomao, R., Álvarez, C., Mehta, Y., Higuera, F., Cuellar, L. E., Arikan, Ö. A., Abouqal, R., & Leblebicioğlu, H. (2006) · Annals of Internal Medicine · doi:10.7326/0003-4819-145-8-200610170-00007 · Cited by 544 · see citing works
Oral Decontamination with Chlorhexidine Reduces the Incidence of Ventilator-associated Pneumonia
Koeman, M., van der Ven, A. J. A. M., Hak, E., Joore, H. C. A., Kaasjager, K. A. H., de Smet, A. M. G. A., Ramsay, G., Dormans, T. P. J., Aarts, L. P. H. J., de Bel, E. E., et al. (2006) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.200505-820oc · Cited by 410 · see citing works
Safdar, N., Crnich, C. J., & Maki, D. G. (2005) · Respiratory Care · doi:10.4187/respcare.05500725 · Cited by 368 · see citing works
Oral biofilms, periodontitis, and pulmonary infections
Paju, S., & Scannapieco, F. A. (2007) · Oral Diseases · doi:10.1111/j.1601-0825.2007.01410a.x · Cited by 368 · see citing works
Use of checkerboard DNA–DNA hybridization to study complex microbial ecosystems
Socransky, S. S., Haffajee, A. D., Smith, C., Martin, L., Haffajee, J. A., Uzel, N. G., & Goodson, J. M. (2004) · Oral Microbiology and Immunology · doi:10.1111/j.1399-302x.2004.00168.x · Cited by 343 · see citing works
Nosocomial Bronchopneumonia in the Critically III: Histologic and Bacteriologic Aspects
Rouby, J., de Lassale, E. M., Poète, P., Nicolas-Chanoine, M.-H., Bodin, L., Jarlier, V., Charpentier, Y. L., Grosset, J. H. E., & VIARS, P. (1992) · American Review of Respiratory Disease · doi:10.1164/ajrccm/146.4.1059 · Cited by 333 · see citing works
Colonization of dental plaque: A source of nosocomial infections in intensive care unit patients
Fourrier, F., Duvivier, B., Boutigny, H., Roussel‐Delvallez, M., & Chopin, C. (1998) · Critical Care Medicine · doi:10.1097/00003246-199802000-00032 · Cited by 292 · see citing works
Oral health and care in the intensive care unit: state of the science
Munro, C. L., & Grap, M. J. (2004) · American Journal of Critical Care · doi:10.4037/ajcc2004.13.1.25 · Cited by 276 · see citing works
Topical chlorhexidine for prevention of ventilator-associated pneumonia: A meta-analysis*
Chlebicki, M. P., & Safdar, N. (2007) · Critical Care Medicine · doi:10.1097/01.ccm.0000253395.70708.ac · Cited by 229 · see citing works
Heo, S., Haase, E. M., Lesse, A. J., Gill, S. R., & Scannapieco, F. A. (2008) · Clinical Infectious Diseases · doi:10.1086/593193 · Cited by 189 · see citing works
Bahrani-Mougeot, F. K., Paster, B. J., Coleman, S., Barbuto, S., Brennan, M. T., Noll, J., Kennedy, T., Fox, P. C., & Lockhart, P. B. (2007) · Journal of Clinical Microbiology · doi:10.1128/jcm.01963-06 · Cited by 176 · see citing works
Luna, C. M., Videla, A. J., Mattera, J., Vay, C. A., Famiglietti, A. M. R., Vujacich, P., & Niederman, M. S. (1999) · CHEST Journal · doi:10.1378/chest.116.4.1075 · Cited by 135 · see citing works
Oral Care in the Intensive Care Unit: A Review
Abidia, R. F. (2007) · The Journal of Contemporary Dental Practice · doi:10.5005/jcdp-8-1-76 · Cited by 134 · see citing works
Rapid method for the purification of DNA from subgingival microorganisms
Smith, G. L., Socransky, S. S., & Smith, C. S. (1989) · Oral Microbiology and Immunology · doi:10.1111/j.1399-302x.1989.tb00406.x · Cited by 124 · see citing works
Sona, C. S., Zack, J. E., Schallom, M. E., McSweeney, M., McMullen, K., Thomas, J., Coopersmith, C. M., Boyle, W. A., Buchman, T. G., Mazuski, J. E., et al. (2008) · Journal of Intensive Care Medicine · doi:10.1177/0885066608326972 · Cited by 109 · see citing works
Pneumonia nosocomial: importância do microambiente oral
Amaral, S. M., Côrtes, A. D. Q., & Pires, F. R. (2009) · Jornal Brasileiro de Pneumologia · doi:10.1590/s1806-37132009001100010 · Cited by 76 · see citing works
Higiene bucal com clorexidina na prevenção de pneumonia associada à ventilação mecânica
Beraldo, C. C., & de Andrade, D. (2008) · Jornal Brasileiro de Pneumologia · doi:10.1590/s1806-37132008000900012 · Cited by 66 · see citing works
Woske, H.-J., Röding, T., Schulz, I., et al. (2001) · Critical Care · doi:10.1186/cc1017 · Cited by 65 · see citing works
The role of oral microbial colonization in ventilator-associated pneumonia
Brennan, M. T., Bahrani‐Mougeot, F. K., Fox, P. C., Kennedy, T. P., Hopkins, S., Boucher, R. C., & Lockhart, P. B. (2004) · Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology · doi:10.1016/j.tripleo.2004.06.005 · Cited by 63 · see citing works
A importância da atuação odontológica em pacientes internados em unidade de terapia intensiva
de Morais, T. M. N., da Silva, A., Avi, A. L. R. D. O., et al. (2006) · Revista Brasileira de Terapia Intensiva · doi:10.1590/s0103-507x2006000400016 · Cited by 50 · see citing works
Niederman, M. S. (2010) · Clinical Infectious Diseases · doi:10.1086/653055 · Cited by 47 · see citing works
Respiratory nosocomial infections in the medical intensive care unit
Cavalcanti, M. A. D. N., Valencia, M., & Torres, A. (2005) · Microbes and Infection · doi:10.1016/j.micinf.2004.12.001 · Cited by 46 · see citing works
Wood, G. C., Mueller, E. W., Croce, M. A., et al. (2005) · Surgical Infections · doi:10.1089/sur.2005.6.203 · Cited by 35 · see citing works
Efficacy of Bilateral Bronchoalveolar Lavage for Diagnosis of Ventilator-Associated Pneumonia
Zaccard, C. R., Schell, R. F., & Spiegel, C. A. (2009) · Journal of Clinical Microbiology · doi:10.1128/jcm.00747-09 · Cited by 28 · see citing works
New approaches to prevention and treatment of nosocomial pneumonia.
Bassin, A. S., & Niederman, M. S. (1995) · PubMed · Cited by 18 · see citing works
Prevention of Ventilator-Associated Pneumonia by Use of Oral Chlorhexidine
Silvestri, L., van Saene, H. K. F., Milanese, M., et al. (2008) · Infection Control and Hospital Epidemiology · doi:10.1086/591742 · Cited by 12 · see citing works
Flagged first, then by citations
Research works printed but not linked: 2
- Amaral, S. M., Cortes Ade, Q., & Pires, F. R. (2009). Nosocomial pneumonia: Importance of the oral environment. Journal Brasileiro de Pneumologia, 35(11), 1116-1124.
- Beraldo, C. C., & Andrade, D. (2008). Oral hygiene with chlorhexidine in preventing pneumonia associated with mechanical ventilation. Journal Brasileiro de Pneumologia, 34(9), 707-714.
Cited by
Sorted by citations
Oral Microbiome and SARS-CoV-2: Beware of Lung Co-infection
Bao, L., Zhang, C., Dong, J., Zhao, L., Li, Y., & Sun, J. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.01840 · Cited by 207 · see citing works
- Authors
- de Carvalho Baptista, I. M., Martinho, F. C., Nascimento, G. G., da Rocha Santos, C. E., Prado, R. F. D., & Valera, M. C.
- Affiliations
- Universidade Estadual Paulista (Unesp) (BR)
- Aarhus University (DK)
- Corresponding author
- Prado, Renata Falchete do
- Venue
- Archives of Oral Biology
- Publisher
- Elsevier BV
- Published
- 2018-01
- Volume
- 85
- Pages
- 64-69
- ISSN
- 0003-9969 (ISSN-L)
- OpenAlex
- W2762038834
- Cited by
- 58
- References
- 27
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 7 · 2023: 7 · 2022: 14 · 2021: 11 · 2020: 5 · 2019: 2 · 2018: 1
- FWCI
- 2.05
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Archives of Oral Biology (publishedVersion)
- PubMed (publishedVersion)
- Americanae (AECID Library) (publishedVersion, other-oa)
- Scopus (Elsevier) (submittedVersion)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
- Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo (2014/, 14/25789-9)
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Universidade Estadual Paulista
- Topics
- Health Sciences › Medicine › Critical Care and Intensive Care Medicine › Nosocomial Infections in ICU
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Keywords
- ventilator-associated pneumonia, oropharyngeal colonization, lower respiratory tract colonization, mechanical ventilation, orotracheal intubation, oral bacteria, checkerboard DNA-DNA hybridization, blood culture, Enterococcus faecalis, Fusobacterium periodonticum, Treponema, Streptococcus, Neisseria mucosa, Propionibacterium acnes, Veillonella parvula, bacterial diversity, intensive care unit patients
- MeSH terms
- Adult, Aged, Aged, 80 and over, Female, Humans, Intensive Care Units, Intubation, Intratracheal, Lung, Male, Middle Aged, Mouth, Oropharynx, Risk Factors, DNA Probes, Bronchoalveolar Lavage, Pneumonia, Ventilator-Associated
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
