The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Distribution of macrophage polarization markers in human atherosclerosis
Open accessAbstract
OBJECTIVE Macrophages are decisive in the chronic inflammatory processes that drive atherogenesis. The purpose of this study was to explore the presence and spatial distribution of polarized macrophage populations in human atherosclerosis. METHODS & RESULTS We used transcriptomics and immunohistochemistry to analyze macrophage subset dynamics in successive stages of atherogenesis. Developing lesions progressively accumulated both M1 and M2 cells, as was signified by the enhanced expression of associated markers at the transcriptional and protein level. Histologically, these markers were confined to overlapping, but spatially distinct CD68(+) areas of the intima. We subsequently quantified the presence of these markers in relation to morphological determinants of plaque stability. In line with their pro-inflammatory characteristics, M1 macrophages dominated the rupture-prone shoulder regions of the plaque over M2 polarized cells, while the fibrous caps of lesions showed no significant differences between subsets. In contrast, vascular adventitial tissue displayed a pronounced M2 activation profile. As expected, areas of intraplaque hemorrhage clearly associated with CD163 staining. Rather than being limited to complicated lesions, this M2 marker was also readily detectable in stable plaques. Finally, foamy macrophages displayed an ambiguous repertoire that incorporates individual M1 and M2 markers. CONCLUSION M1 and M2 macrophage populations are present throughout atherogenesis. These subsets display disparity when it comes to their prevalence in morphological compartments of the vessel wall. Our current findings warrant continued investigation into the functional implications of polarized macrophage populations in human atherosclerosis.
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Cites
Atherosclerosis--an inflammatory disease
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Obesity induces a phenotypic switch in adipose tissue macrophage polarization
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Macrophage-specific PPARγ controls alternative activation and improves insulin resistance
Odegaard, J. I., Ricardo-Gonzalez, R. R., Goforth, M. H., Morel, C. R., Subramanian, V., Mukundan, L., Eagle, A. R., Vats, D., Brombacher, F., Ferrante, A. W., et al. (2007) · Nature · doi:10.1038/nature05894 · Cited by 2,107 · cited 1×
“Notably, adipose tissue macrophages assist healthy metabolism under lean conditions through their anti-inflammatory M2 properties [30].”
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“Although in our view this does not negate CD163’s ability to signify an unstable plaque phenotype [20], these results contribute to the ongoing debate concerning the function of CD163þ macrophages in in vivo disease models [27].”
ResultMoulton, K. S., Vakili, K., Zurakowski, D., Soliman, M., Butterfield, C., Sylvin, E., Lo, K.-M., Gillies, S., Javaherian, K., & Folkman, J. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0730843100 · Cited by 652
Macrophage plasticity in experimental atherosclerosis
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Chinetti-Gbaguidi, G., Baron, M., Bouhlel, M. A., Vanhoutte, J., Copin, C., Sebti, Y., Derudas, B., Mayi, T., Bories, G., Tailleux, A., et al. (2011) · Circulation Research · doi:10.1161/circresaha.110.233775 · Cited by 387
Multiple Immunoenzyme Staining: Methods and Visualizations for the Observation With Spectral Imaging
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Cited by
Mechanisms of plaque formation and rupture
Bentzon, J. F., Otsuka, F., Virmani, R., & Falk, E. (2014) · Circulation Research · doi:10.1161/circresaha.114.302721 · Cited by 2,391
Macrophage Polarization States in the Tumor Microenvironment
Boutilier, A. J., & Elsawa, S. F. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22136995 · Cited by 1,631
Modulation of macrophage phenotype by cell shape
McWhorter, F. Y., Wang, T., Nguyen, P., Chung, T., & Liu, W. F. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1308887110 · Cited by 1,482
Macrophage Immunometabolism: Where Are We (Going)?
Van den Bossche, J., O’Neill, L. A., & Menon, D. (2017) · Trends in Immunology · doi:10.1016/j.it.2017.03.001 · Cited by 1,163
Mitochondrial dysfunction prevents repolarization of inflammatory macrophages
Van den Bossche, J., Baardman, J., Otto, N. A., van der Velden, S., Neele, A. E., van den Berg, S. M., Luque-Martin, R., Chen, H.-J., Boshuizen, M. C. S., Ahmed, M., et al. (2016) · Cell Reports · doi:10.1016/j.celrep.2016.09.008 · Cited by 1,027
Metadata
- Authors
- Stöger, J. L., Gijbels, M. J. J., van der Velden, S., Manca, M., van der Loos, C. M., Biessen, E. A. L., Daemen, M. J. A. P., Lutgens, E., & de Winther, M. P. J.
- Venue
- Atherosclerosis
- Publisher
- Elsevier BV
- Published
- 2012-12
- Volume
- 225
- Issue
- 2
- Pages
- 461-468
- ISSN
- 0021-9150 (ISSN-L)
- OpenAlex
- W2057835664
- Cited by
- 621
- References
- 10
- FWCI
- 12.57
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Stöger, J. L., Gijbels, M. J. J., van der Velden, S., Manca, M., van der Loos, C. M., Biessen, E. A. L., Daemen, M. J. A. P., Lutgens, E., & de Winther, M. P. J. (2012). Distribution of macrophage polarization markers in human atherosclerosis. Atherosclerosis. https://doi.org/10.1016/j.atherosclerosis.2012.09.013
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