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Sex-specific metabolic and functional differences in human umbilical vein endothelial cells from twin pairs

Lorenz, Mario, Blaschke, Benjamin, Benn, Andreas, Hammer, Elke, Witt, Eric, Kirwan, Jennifer, Fritsche-Guenther, Raphaela, Gloaguen, Yoann, Bartsch, Cornelia, Vietzke, Angelika, Kramer, Frederike, Kappert, Kai, Brunner, Patrizia, Nguyen, Hoang Giang, Dreger, Henryk, Stangl, Karl, Knaus, Petra, Stangl, Verena·2019·Atherosclerosis
DOI 10.1016/j.atherosclerosis.2019.10.007
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Abstract

Background and aims Gonadal hormones are mainly thought to account for sex and gender differences in the incidence, clinical manifestation and therapy of many cardiovascular diseases. However, intrinsic sex differences at the cellular level are mostly overlooked. Here, we assessed sex-specific metabolic and functional differences between male and female human umbilical vein endothelial cells (HUVECs). Methods Cellular metabolism was investigated by bioenergetic studies (Seahorse Analyser) and a metabolomic approach. Protein levels were determined by Western blots and proteome analysis. Vascular endothelial growth factor (VEGF)-stimulated cellular migration was assessed by gap closure. HUVECs from dizygotic twin pairs were used for most experiments. Results No sex differences were observed in untreated cells. However, sexual dimorphisms appeared after stressing the cells by serum starvation and treatment with VEGF. Under both conditions, female cells had higher intracellular ATP and metabolite levels. A significant decline in ATP levels was observed in male cells after serum starvation. After VEGF, the ratio of glycolysis/mitochondrial respiration was higher in female cells and migration was more pronounced. Conclusions These results point to an increased stress tolerance of female cells. We therefore propose that female cells have an energetic advantage over male cells under conditions of diminished nutrient supply. A more favourable energy balance of female HUVECs after seru…

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Cited by

Metadata

Authors
Lorenz, M., Blaschke, B., Benn, A., Hammer, E., Witt, E., Kirwan, J., Fritsche-Guenther, R., Gloaguen, Y., Bartsch, C., Vietzke, A., Kramer, F., Kappert, K., Brunner, P., Nguyen, H. G., Dreger, H., Stangl, K., Knaus, P., & Stangl, V.
ORCID
Venue
Atherosclerosis
Publisher
Elsevier BV
Published
2019-12
Volume
291
Pages
99-106
ISSN
0021-9150
DOI
10.1016/j.atherosclerosis.2019.10.007
Cited by
39
References
0
Language
en
Licence
Funders
  • Deutsches Zentrum für Herz-Kreislaufforschung
  • Deutsche Forschungsgemeinschaft
  • Bundesministerium für Bildung und Forschung (81X2100211)

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