Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 70

The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

Does cellular sex matter? Dimorphic transcriptional differences between female and male endothelial cells

Lorenz, Mario, Koschate, Janina, Kaufmann, Katharina, Kreye, Corinna, Mertens, Michael, Kuebler, Wolfgang M., Baumann, Gert, Gossing, Gabriele, Marki, Alex, Zakrzewicz, Andreas, Miéville, Christian, Benn, Andreas, Horbelt, Daniel, Wratil, Paul R., Stangl, Karl, Stangl, Verena·2015·Atherosclerosis
DOI 10.1016/j.atherosclerosis.2015.02.018
Open this paper in CoChat — free account

Abstract

Objective Significant sex differences exist in cardiovascular diseases. Although an impact of gonadal hormones is presumed, it is largely unknown whether sexually dimorphic gene expression also plays a role and whether cells themselves show intrinsic sex differences. Methods We performed whole genome expression analyses in human umbilical vein endothelial cells (HUVEC) from 20 male and 20 female donors and compared levels of gene transcription between the sexes. To further assess whether there is a sex-specific response to stress, we subjected male and female HUVEC to shear for 24 h and analysed changes in gene expression. Results Genes indicative for greater immune responsiveness were stronger expressed in female compared to male HUVEC. There was a significant enrichment of 77 immune-related genes in female HUVEC. These increased transcriptional levels in female cells were verified for 20 genes by real-time RT-PCR. 6.7% of all mRNAs were regulated by shear stress. Female HUVEC showed a more pronounced transcriptional response to shear than did their male counterparts. In addition to quantitative differences, a number of genes were regulated in the opposite direction between the two sexes by shear stress. Functionally, female HUVEC showed a higher cell viability after serum starvation and an increased tube formation capacity compared to male cells. Conclusion These findings underscore the importance for differentiation between male and female cells in cell culture experiments…

Overview

Integrity signals

Registry

Crossref · 10.1016/j.atherosclerosis.2015.02.018

Cites

3 of 3 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 3 references

Research works printed but not linked: 41

  1. Dunlay, 2012, Gender differences in the pathophysiology, clinical presentation, and outcomes of ischemic heart failure, Curr. Heart Fail. Rep., 9, 267, 10.1007/s11897-012-0107-7 · DOI
  2. Stangl, 2008, Current diagnostic concepts to detect coronary artery disease in women, Eur. Heart J., 29, 707, 10.1093/eurheartj/ehn047 · DOI
  3. Jochmann, 2005, Female-specific aspects in the pharmacotherapy of chronic cardiovascular diseases, Eur. Heart J., 26, 1585, 10.1093/eurheartj/ehi397 · DOI
  4. Regitz-Zagrosek, 2013, Sex and sex hormone-dependent cardiovascular stress responses, Hypertension, 61, 270, 10.1161/HYPERTENSIONAHA.111.189233 · DOI
  5. Akishita, 2012, Hormonal effects on blood vessels, Hypertens. Res., 35, 363, 10.1038/hr.2012.4 · DOI
  6. Knowlton, 2012, Estrogen and the cardiovascular system, Pharmacol. Ther., 135, 54, 10.1016/j.pharmthera.2012.03.007 · DOI
  7. Straface, 2012, Sex differences at cellular level: “cells have a sex”, Handb. Exp. Pharmacol., 214, 49, 10.1007/978-3-642-30726-3_3 · DOI
  8. Maselli, 2009, Cell sex: a new look at cell fate studies, FASEB J., 23, 978, 10.1096/fj.08-114348 · DOI
  9. Wang, 2010, Intrinsic sex-specific differences in microvascular endothelial cell phosphodiesterases, Am. J. Physiol. Heart Circ. Physiol., 298, H1146, 10.1152/ajpheart.00252.2009 · DOI
  10. Heidecker, 2010, The gene expression profile of patients with new-onset heart failure reveals important gender-specific differences, Eur. Heart J., 31, 1188, 10.1093/eurheartj/ehp549 · DOI
  11. Johnson, 2011, Mechanotransduction of shear in the endothelium: basic studies and clinical implications, Vasc. Med., 16, 365, 10.1177/1358863X11422109 · DOI
  12. Stangl, 2001, Homocysteine inhibits TNF-alpha-induced endothelial adhesion molecule expression and monocyte adhesion via nuclear factor-kappaB dependent pathway, Biochem. Biophys. Res. Commun., 280, 1093, 10.1006/bbrc.2000.4207 · DOI
  13. Bongrazio, 2000, Evidence for modulation of genes involved in vascular adaptation by prolonged exposure of endothelial cells to shear stress, Cardiovasc Res., 47, 384, 10.1016/S0008-6363(00)00111-5 · DOI
  14. Robinson, 2013, The IMGT/HLA database, Nucleic Acids Res., 41, D1222, 10.1093/nar/gks949 · DOI
  15. Pollitzer, 2013, Biology: cell sex matters, Nature, 500, 23, 10.1038/500023a · DOI
  16. Ritz, 2014, First steps for integrating sex and gender considerations into basic experimental biomedical research, FASEB J., 28, 4, 10.1096/fj.13-233395 · DOI
  17. Horton, 2004, Gene map of the extended human MHC, Nat. Rev. Genet., 5, 889, 10.1038/nrg1489 · DOI
  18. Lidington, 1999, A comparison of primary endothelial cells and endothelial cell lines for studies of immune interactions, Transpl. Immunol., 7, 239, 10.1016/S0966-3274(99)80008-2 · DOI
  19. Barry, 2011, Meta-analysis of sex difference in testosterone levels in umbilical cord blood, J. Obstet. Gynaecol., 31, 697, 10.3109/01443615.2011.614971 · DOI
  20. van de Beek, 2004, Relationships between sex hormones assessed in amniotic fluid, and maternal and umbilical cord serum: what is the best source of information to investigate the effects of fetal hormonal exposure?, Horm. Behav., 46, 663, 10.1016/j.yhbeh.2004.06.010 · DOI
  21. Furuhashi, 1982, Concentrations of luteinizing hormone-human chorionic gonadotropin, beta subunit of human chorionic gonadotropin, follicle-stimulating hormone, estradiol, cortisol, and testosterone in cord sera and their correlations, Am. J. Obstet. Gynecol., 143, 918, 10.1016/0002-9378(82)90474-4 · DOI
  22. Isensee, 2008, Sexually dimorphic gene expression in the heart of mice and men, J. Mol. Med. (Berl), 86, 61, 10.1007/s00109-007-0240-z · DOI
  23. Vawter, 2004, Gender-specific gene expression in post-mortem human brain: localization to sex chromosomes, Neuropsychopharmacology, 29, 373, 10.1038/sj.npp.1300337 · DOI
  24. Berchtold, 2008, Gene expression changes in the course of normal brain aging are sexually dimorphic, Proc. Natl. Acad. Sci. U. S. A., 105, 15605, 10.1073/pnas.0806883105 · DOI · Paper page
  25. Hager, 2008, Sex dependent imprinting effects on complex traits in mice, BMC Evol. Biol., 8, 303, 10.1186/1471-2148-8-303 · DOI
  26. Gregg, 2010, Sex-specific parent-of-origin allelic expression in the mouse brain, Science, 329, 682, 10.1126/science.1190831 · DOI
  27. Evangelista, 2013, miR-222 contributes to sex-dimorphic cardiac eNOS expression via ets-1, Physiol. Genomics, 45, 493, 10.1152/physiolgenomics.00008.2013 · DOI
  28. Glass, 2001, The road ahead, Cell, 104, 503, 10.1016/S0092-8674(01)00238-0 · DOI
  29. Nussinovitch, 2012, The role of gender and organ specific autoimmunity, Autoimmun. Rev., 11, A377, 10.1016/j.autrev.2011.11.001 · DOI
  30. Gleicher, 2007, Gender as risk factor for autoimmune diseases, J. Autoimmun., 28, 1, 10.1016/j.jaut.2006.12.004 · DOI
  31. Bouman, 2005, Sex hormones and the immune response in humans, Hum. Reprod. Update, 11, 411, 10.1093/humupd/dmi008 · DOI · Paper page
  32. Cutolo, 2010, The immunomodulatory effects of estrogens: clinical relevance in immune-mediated rheumatic diseases, Ann. N. Y. Acad. Sci., 1193, 36, 10.1111/j.1749-6632.2009.05383.x · DOI
  33. McCormick, 2001, DNA microarray reveals changes in gene expression of shear stressed human umbilical vein endothelial cells, Proc. Natl. Acad. Sci. U. S. A., 98, 8955, 10.1073/pnas.171259298 · DOI
  34. Fledderus, 2007, Prolonged shear stress and KLF2 suppress constitutive proinflammatory transcription through inhibition of ATF2, Blood, 109, 4249, 10.1182/blood-2006-07-036020 · DOI
  35. Matarrese, 2011, Gender disparity in susceptibility to oxidative stress and apoptosis induced by autoantibodies specific to RLIP76 in vascular cells, Antioxid. Redox Signal., 15, 2825, 10.1089/ars.2011.3942 · DOI
  36. Arnold, 2012, Understanding the sexome: measuring and reporting sex differences in gene systems, Endocrinology, 153, 2551, 10.1210/en.2011-2134 · DOI
  37. Tian, 2012, Effects of gender on gene expression in the blood of ischemic stroke patients, J. Cereb. Blood Flow Metab., 32, 780, 10.1038/jcbfm.2011.179 · DOI
  38. Lista, 2011, On the role of autophagy in human diseases: a gender perspective, J. Cell. Mol. Med., 15, 1443, 10.1111/j.1582-4934.2011.01293.x · DOI
  39. Pierdominici, 2011, Gender specific aspects of cell death in the cardiovascular system, Curr. Pharm. Des., 17, 1046, 10.2174/138161211795656891 · DOI
  40. Straface, 2009, Cell sex determines anoikis resistance in vascular smooth muscle cells, FEBS Lett., 583, 3448, 10.1016/j.febslet.2009.09.052 · DOI
  41. Addis, 2014, Human umbilical endothelial cells (HUVECs) have a sex: characterisation of the phenotype of male and female cells, Biol. Sex Differ., 5, 18, 10.1186/s13293-014-0018-2 · DOI · Paper page

Cited by

See all 70

Metadata

Authors
Lorenz, M., Koschate, J., Kaufmann, K., Kreye, C., Mertens, M., Kuebler, W. M., Baumann, G., Gossing, G., Marki, A., Zakrzewicz, A., Miéville, C., Benn, A., Horbelt, D., Wratil, P. R., Stangl, K., & Stangl, V.
Venue
Atherosclerosis
Publisher
Elsevier BV
Published
2015-05
Volume
240
Issue
1
Pages
61-72
ISSN
0021-9150
DOI
10.1016/j.atherosclerosis.2015.02.018
Cited by
70
References
3
Language
en
Licence

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Does cellular sex matter? Dimorphic transcriptional differences between female and male endothelial cells. https://app.cochat.ai/papers/10.1016/j.atherosclerosis.2015.02.018
The paper
Lorenz, M., Koschate, J., Kaufmann, K., Kreye, C., Mertens, M., Kuebler, W. M., Baumann, G., Gossing, G., Marki, A., Zakrzewicz, A., Miéville, C., Benn, A., Horbelt, D., Wratil, P. R., Stangl, K., & Stangl, V. (2015). Does cellular sex matter? Dimorphic transcriptional differences between female and male endothelial cells. Atherosclerosis. https://doi.org/10.1016/j.atherosclerosis.2015.02.018