Cited by 120ATP Synthase and ATPases ResearchProtein Structure and DynamicsForce Microscopy Techniques and Applications

The mechanism of rotating proton pumping ATPases

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Nakanishi-Matsui, Mayumi, Sekiya, Mizuki, Nakamoto, Robert K., Futai, Masamitsu·2010·Biochimica et Biophysica Acta (BBA) - Bioenergetics

Abstract

Two proton pumps, the F-ATPase (ATP synthase, FoF1) and the V-ATPase (endomembrane proton pump), have different physiological functions, but are similar in subunit structure and mechanism. They are composed of a membrane extrinsic (F1 or V1) and a membrane intrinsic (Fo or Vo) sector, and couple catalysis of ATP synthesis or hydrolysis to proton transport by a rotational mechanism. The mechanism of rotation has been extensively studied by kinetic, thermodynamic and physiological approaches. Techniques for observing subunit rotation have been developed. Observations of micron-length actin filaments, or polystyrene or gold beads attached to rotor subunits have been highly informative of the rotational behavior of ATP hydrolysis-driven rotation. Single molecule FRET experiments between fluorescent probes attached to rotor and stator subunits have been used effectively in monitoring proton motive force-driven rotation in the ATP synthesis reaction. By using small gold beads with diameters of 40-60 nm, the E. coli F1 sector was found to rotate at surprisingly high speeds (>400 rps). This experimental system was used to assess the kinetics and thermodynamics of mutant enzymes. The results revealed that the enzymatic reaction steps and the timing of the domain interactions among the beta subunits, or between the beta and gamma subunits, are coordinated in a manner that lowers the activation energy for all steps and avoids deep energy wells through the rotationally-coupled steady-state reaction. In this review, we focus on the mechanism of steady-state F1-ATPase rotation, which maximizes the coupling efficiency between catalysis and rotation.

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Crossref · 10.1016/j.bbabio.2010.02.014

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  1. S. Lecchi, C.W. Slayman, Yeast plasma-membrane H + -ATPase: model system for studies of structure, function, biogenetics, and regulation, in: M. Futai, Y. Wada, J. Kaplan (Eds.), Handbook of ATPases : Biochemistry, Cell Biology, Pathophys- iology, 2004, pp. 3-24.

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Authors
Nakanishi-Matsui, M., Sekiya, M., Nakamoto, R. K., & Futai, M.
Affiliations
  • Iwate Medical University (JP)
  • University of Virginia (US)
Venue
Biochimica et Biophysica Acta (BBA) - Bioenergetics
Publisher
Elsevier BV
Published
2010-08
Volume
1797
Issue
8
Pages
1343-1352
ISSN
0005-2728 (ISSN-L)
OpenAlex
W2140969249
Cited by
120
References
103
Citations by year
2026: 3 · 2025: 2 · 2024: 3 · 2023: 23 · 2022: 2 · 2021: 8 · 2020: 7 · 2019: 2 · 2018: 5 · 2017: 6 · 2016: 8 · 2015: 7 · 2014: 7 · 2013: 10 · 2012: 11
FWCI
1.04
Type
journal article
Language
en
Open access
Locations
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • CREST (Core Research for Evolutional Science and Technology)
  • JST (Japan Science and Technology Agency)
  • Japanese Ministry of Education, Culture, and Science
  • Terumo Life Science Foundation
  • Japan Foundation for Applied Enzymology
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › ATP Synthase and ATPases Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
Keywords
F1-ATPase, V-ATPase, proton-pumping ATPase, ATP production, single-molecule FRET, Escherichia coli
MeSH terms
Adenosine Triphosphate, Animals, Catalysis, Proton-Translocating ATPases, Humans, Rotation, Thermodynamics, Vacuolar Proton-Translocating ATPases

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