Cited by 71RNA and protein synthesis mechanismsRNA modifications and cancerBacterial Genetics and Biotechnology

Amino acid recognition and gene regulation by riboswitches

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Serganov, Alexander, Patel, Dinshaw J.·2009·Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms

Abstract

Riboswitches specifically control expression of genes predominantly involved in biosynthesis, catabolism and transport of various cellular metabolites in organisms from all three kingdoms of life. Among many classes of identified riboswitches, two riboswitches respond to amino acids lysine and glycine to date. Though these riboswitches recognize small compounds, they both belong to the largest riboswitches and have unique structural and functional characteristics. In this review, we attempt to characterize molecular recognition principles employed by amino acid-responsive riboswitches to selectively bind their cognate ligands and to effectively perform a gene regulation function. We summarize up-to-date biochemical and genetic data available for the lysine and glycine riboswitches and correlate these results with recent high-resolution structural information obtained for the lysine riboswitch. We also discuss the contribution of lysine riboswitches to antibiotic resistance and outline potential applications of riboswitches in biotechnology and medicine.

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Crossref · 10.1016/j.bbagrm.2009.07.002

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  • Direct recognition of amino acids by RNA molecules survives in other functional spheres of modern cells, in particular, as amino acid-binding riboswitches.

    “It seems interesting to note that, although direct recognition of amino acids by RNA molecules seems to be banished from the modern translation system, it survives in other functional spheres of modern cells, in particular, as amino acid-binding riboswitches [96,97].”

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Authors
Serganov, A., & Patel, D. J.
Affiliations
Memorial Sloan Kettering Cancer Center (US)
Corresponding author
Serganov, Alexander
Venue
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms
Publisher
Elsevier BV
Published
2009-09
Volume
1789
Issue
9-10
Pages
592-611
ISSN
1874-9399 (ISSN-L)
OpenAlex
W1969810565
Cited by
69
References
176
Citations by year
2026: 1 · 2025: 3 · 2024: 6 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 3 · 2019: 3 · 2018: 4 · 2017: 7 · 2016: 3 · 2015: 5 · 2014: 3 · 2013: 1 · 2012: 3
FWCI
0.51
Type
journal article
Language
en
Open access
Locations
  • Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institute of General Medical Sciences (GM073618, R01GM073618, R01GM034504)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA modifications and cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
riboswitches, amino acid recognition, glycine riboswitch, ligand binding, gene expression regulation, riboswitch structure, antibiotic resistance
MeSH terms
Amino Acids, Bacillus subtilis, Base Sequence, Binding Sites, Biochemistry, Glycine, Hydrogen Bonding, Ligands, Lysine, Molecular Sequence Data, Nucleic Acid Conformation, Phylogeny, Ribosomes, RNA, RNA, Bacterial, Gene Expression Regulation, Bacterial, Crystallography, X-Ray, 5' Untranslated Regions

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